Colony Features of Pasteurella multocida and Their Use in Diagnosing Fowl Cholera in Turkeys

Re, Bond; Jm, Donahue; Ld, Olson

doi:10.2307/1588552

Cited by 22 publications

(3 citation statements)

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“…When the extent of biofilm formation was compared with colony morphology under iridescent light, it was noted that there was an inverse relationship between the iridescence and how mucoid the colonies were and the amount of biofilm formed by that strain. It has been clearly established that cells with the most CPS on their surface form the most mucoid, iridescent colonies, with smooth colonies containing less CPS and smaller blue colonies (rough) being CPS deficient ( 47 , 48 ). Inverse correlations between CPS content and biofilm formation that are similar to the results described here for P. multocida have also been reported for Neisseria meningitidis ( 49 ), Escherichia coli ( 50 , 51 ), and other bacterial species ( 49 , 52 ).…”

Section: Discussionmentioning

confidence: 99%

“…Immunoassays are unavailable because antibodies against the type A CPS hyaluronic acid are difficult to obtain because this CPS is recognized as “self” by the immune system. An alternative method to quantify P. multocida type A CPS production is by observing the colony morphology on solid agar medium ( 47 ). This method has been used to distinguish encapsulated, virulent P. multocida from less virulent or avirulent decapsulated P. multocida for almost a century ( 53 ).…”

Section: Discussionmentioning

confidence: 99%

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Capsular Polysaccharide Interferes with Biofilm Formation byPasteurella multocidaSerogroup A

Petruzzi

Briggs

Tatum

et al. 2017

mBio

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Pasteurella multocida is an important multihost animal and zoonotic pathogen that is capable of causing respiratory and multisystemic diseases, bacteremia, and bite wound infections. The glycosaminoglycan capsule of P. multocida is an essential virulence factor that protects the bacterium from host defenses. However, chronic infections (such as swine atrophic rhinitis and the carrier state in birds and other animals) may be associated with biofilm formation, which has not been characterized in P. multocida. Biofilm formation by clinical isolates was inversely related to capsule production and was confirmed with capsule-deficient mutants of highly encapsulated strains. Capsule-deficient mutants formed biofilms with a larger biomass that was thicker and smoother than the biofilm of encapsulated strains. Passage of a highly encapsulated, poor-biofilm-forming strain under conditions that favored biofilm formation resulted in the production of less capsular polysaccharide and a more robust biofilm, as did addition of hyaluronidase to the growth medium of all of the strains tested. The matrix material of the biofilm was composed predominately of a glycogen exopolysaccharide (EPS), as determined by gas chromatography-mass spectrometry, nuclear magnetic resonance, and enzymatic digestion. However, a putative glycogen synthesis locus was not differentially regulated when the bacteria were grown as a biofilm or planktonically, as determined by quantitative reverse transcriptase PCR. Therefore, the negatively charged capsule may interfere with biofilm formation by blocking adherence to a surface or by preventing the EPS matrix from encasing large numbers of bacterial cells. This is the first detailed description of biofilm formation and a glycogen EPS by P. multocida.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Capsular Polysaccharide Interferes with Biofilm Formation byPasteurella multocidaSerogroup A

Petruzzi

Briggs

Tatum

et al. 2017

mBio

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show abstract

“…By this way, the importance of encapsulation in the virulence of P. multocida was earlier established. A presumptive diagnosis of P. multocida from cases of fowl cholera has been suggested based on observing colonies on dextrose starch agar using a stereomicroscope with an oblique source of light (Heddleston et al, 1964;Bond et al, 1970). Highly encapsulated colonies, often from clinical specimen, assume an iridescent phenotype whereas those from a serial laboratory passage appear blue or take an intermediate range.…”

Section: Colony and Biochemical Characteristicsmentioning

confidence: 99%