Colletotrichum fructicola STE50 is required for vegetative growth, asexual reproduction, appressorium formation, pathogenicity and the response to external stress

Chen, Yanying; Liu, Junang; Jiang, Shiqiang; He, Lin; Zhou, Guoying

doi:10.1007/s42161-019-00422-3

Cited by 9 publications

(18 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mycelial plugs (5 mm) from PDA were placed in the center of PDA plates and cultured in incubators set at different temperatures (10,15,20,25,28,30, and 35 • C) for 5 days. Moreover, 5 mm mycelial plugs from PDA were placed in the center of PDA plates adjusted to a range of pH values from 3.0 to 10.0 for 5 days in an incubator at 28 • C. Na 2 HPO 4 -citric acid buffer was used to prepare PDA with pH values of 3.0-8.0, while Na 2 CO 3 -NaHCO 3 buffer was used to prepare PDA at pH values 9.0 and 10.0.…”

Section: Effect Of Temperature and Ph On Mycelial Growthmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Characterization, Pathogenicity and Biological Characterization of Colletotrichum Species Associated with Anthracnose of Camellia yuhsienensis Hu in China

Chen

Jiang

Bao

et al. 2021

Forests

View full text Add to dashboard Cite

Camellia yuhsienensis Hu, a species of tea oil tree with resistance to anthracnose, is widely used to breed disease-resistant Camellia varieties. In 2019, anthracnose symptoms were observed on Ca. yuhsienensis for the first time. However, the species and biological characteristics of Colletotrichum spp. isolated from Ca. yuhsienensis (YX-Colletotrichum spp.) have not been elucidated. In this study, five isolates (YX2-5-2, 2YX-3-1, 2YX-5-1, 2YX-8-1-1 and 2YX-8-1-2), which were consistent with the morphological characteristics of Colletotrichum spp., were obtained from Ca. yuhsienensis. A phylogenetic analysis demonstrated that YX2-5-2, 2YX-3-1 and 2YX-8-1-2 belonged to first clade along with Colletotrichum fructicola. 2YX-8-1-1 belonged to the second clade along with Colletotrichum siamense. 2YX-5-1 belonged to the third clade with Colletotrichum camelliae. Pathogenicity tests revealed that the pathogenicity of YX-Colletotrichum spp. was stronger than that of Colletotrichum spp. isolated from Camellia oleifera (GD-Colletotrichum spp.). Biological characteristics illustrated that the mycelial growth of YX-Co. camelliae (2YX-5-1) was slower than that of GD-Co. camelliae when the temperature exceeded 20 °C. In addition, in the presence of ions, the mycelial growth of YX-Co. fructicola (YX2-5-2) and YX-Co. siamense (2YX-8-1-1) was also slower than that of GD-Co. fructicola and GD-Co. siamense. Furthermore, the ability of YX-Colletotrichum spp. to utilize lactose and mannitol was weaker than that of GD-Colletotrichum spp., while the ability to utilize NH4+ was generally stronger than that of GD-Colletotrichum spp. This is the first report of anthracnose of Ca. yuhsienensis induced by Co. fructicola, Co. siamense and Co. camelliae in China. These results will provide theoretical guidance for the study of the pathogenesis and control of anthracnose on Ca. yuhsienensis.

show abstract

Section: Effect Of Temperature and Ph On Mycelial Growthmentioning

confidence: 99%

“…Anthracnose of the tea oil tree is an important factor that limits the yield of tea oil [9,10]. Colletotrichum spp.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization, Pathogenicity and Biological Characterization of Colletotrichum Species Associated with Anthracnose of Camellia yuhsienensis Hu in China

Chen

Jiang

Bao

et al. 2021

Forests

View full text Add to dashboard Cite

show abstract

“…oleifera anthracnose. We recently demonstrated that CfSnf1, CfSte50, and CfGcn5 were involved in pathogenicity by regulating the appressorium formation in C. fructicola ( Zhang et al, 2019 , 2021 ; Chen et al, 2020 ). Transcription factor of CfSte12 and the MAP Kinase CfPmk1 are key regulators of pathogenesis of C. fructicola ( Liang et al, 2019 ; Liu et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

The SNARE Protein CfVam7 Is Required for Growth, Endoplasmic Reticulum Stress Response, and Pathogenicity of Colletotrichum fructicola

Zhang

et al. 2021

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

The tea-oil tree Camellia oleifera is native to China and is cultivated in many parts of southern China. This plant has been grown for over 2,000 years, mainly for its high-quality cooking oil. Anthracnose is the main disease of tea-oil tree and results in a huge loss annually. Colletotrichum fructicola is a major pathogen causing anthracnose on tea-oil tree. In a previous study, we characterized that the bZIP transcription factor CfHac1 controlled the development and pathogenicity of C. fructicola. Here, we identified and characterized the function of CfVAM7 gene, which was significantly downregulated at the transcriptional level in the ΔCfhac1 strain under dithiothreitol stress. Targeted gene deletion revealed that CfVam7 is important in growth, pathogenicity, and responses to endoplasmic reticulum-related stresses. Further analysis revealed that CfVam7 is required for appressorium formation and homotypic vacuole fusion, which are important for fungal pathogen invasion. Cytological examinations revealed that CfVam7 is localized to vacuole membranes in the hyphal stage. The Phox homology (PX) and SNARE domains of CfVam7 were indispensable for normal cellular localization and biological function. Taken together, our results suggested that CfVam7-mediated vacuole membrane fusion promotes growth, stress response, and pathogenicity of C. fructicola.

show abstract

“…fructicola were studied. Previously, we had shown that CfSNF1 , CfSTE50 , CfMFS1 and CfHAC1 were involved in regulating growth, stress response and pathogenicity (Chen et al, 2019, 2020; Yao et al, 2019; Zhang et al, 2019). Shang et al (2020) studied the effector molecule CfEC92 of C .…”

Section: Introductionmentioning

confidence: 99%

“…In this preliminary study, the functions of some genes of C. fructicola were studied. Previously, we had shown that CfSNF1, CfSTE50, CfMFS1 and CfHAC1 were involved in regulating growth, stress response and pathogenicity (Chen et al, 2019(Chen et al, , 2020Yao et al, 2019;Zhang et al, 2019). Shang et al (2020) studied the effector molecule CfEC92 of C. fructicola and found that the effector molecule inhibits the immune response of plants in the early stage of infection (Shang et al, 2020).…”

mentioning

confidence: 99%

A HOPS protein, CfVps39, is required for appressorium formation, environmental stress response and vacuolar fusion of Colletotrichum fructicola

Zhang

2021

Forest Pathology

Self Cite

View full text Add to dashboard Cite

The tea‐oil tree Camellia oleifera is native to China and is cultivated in many parts of southern China. Anthracnose on Ca. oleifera is one of the most serious diseases in China. The major causal pathogen of tea‐oil anthracnose is Colletotrichum fructicola, but the detailed molecular mechanisms of its pathogenicity are still largely unclear. Vacuolar protein sorting 39 (Vps39) is a component of the homotypic fusion and vacuole protein sorting (HOPS) complex. To investigate the role of HOPS proteins in the fungal plant anthracnose pathogen C. fructicola, the CfVPS39 gene was deleted. This resulted in reduced mycelial growth, conidial production and complete loss of pathogenicity on tea‐oil leaves and fruits. In addition, ∆Cfvps39 showed increased sensitivity to cell wall stress, osmotic stress and endoplasmic reticulum stress. Further analysis revealed that CfVps39 is required for appressorium formation and homotypic vacuole fusion, both important for fungal pathogen invasion. Our study provides evidence that CfVps39 has a role in conidiation, stress response, appressorium formation, homotypic vacuole fusion and virulence against tea‐oil. This research not only provides the theories for revealing the pathogenic molecular mechanism of C. fructicola, but also has important guiding significance to reveal new fungicide targets to control this devastating disease.

show abstract

Colletotrichum fructicola STE50 is required for vegetative growth, asexual reproduction, appressorium formation, pathogenicity and the response to external stress

Cited by 9 publications

References 29 publications

Molecular Characterization, Pathogenicity and Biological Characterization of Colletotrichum Species Associated with Anthracnose of Camellia yuhsienensis Hu in China

Molecular Characterization, Pathogenicity and Biological Characterization of Colletotrichum Species Associated with Anthracnose of Camellia yuhsienensis Hu in China

The SNARE Protein CfVam7 Is Required for Growth, Endoplasmic Reticulum Stress Response, and Pathogenicity of Colletotrichum fructicola

A HOPS protein, CfVps39, is required for appressorium formation, environmental stress response and vacuolar fusion of Colletotrichum fructicola

Contact Info

Product

Resources

About