Collagenase activity and protein content of sulcular fluid after scaling and occlusal adjustment of teeth with deep periodontal pockets

Abstract

Three teeth from each of 14 adult patients with advanced periodontitis were included in this study. The Test Tooth was an incisor or canine with increased mobility associated with an occlusal interference and a ≥ 5 mm deep pathologic pocket. The Infected Control Tooth was a non‐mobile incisor or canine with a ≥ 5 mm pocket. A non‐mobile incisor or canine with pockets ≤3 mm served as the Healthy Control Tooth. At least 7 d prior to Day 0 the patients were taught an effective oral hygiene regimen and received a supragingival prophylaxis. At Day 0, sulcular fluid for assay of protein content and Collagenase activity was collected from each chosen pocket. Immediately thereafter the Test Teeth of 7 subjects were scaled and root planed and the Test Teeth of 7 subjects occlusally adjusted. At Day 14, the treatments were reversed for the two groups. Sulcular fluid for the assays was again collected at Days 14 and 28. The protein content and collagenase activity in deep pockets was elevated during periodontitis in both mobile and immobile teeth. After establishment of a supragingivally clean oral environment, a rapid decrease of the collagenase activity took place following scaling and root planing of the root surfaces within the periodontal pockets (p≤0.05). Also, occlusal adjustment of the hypermobile teeth with deep pathological pockets reduced the protein content and collagenase activity in sulcular fluid (p ≤ 0.02). There was a further reduction of collagenase activity when occlu‐sally adjusted teeth were scaled and root planed (p≤0.02). No change in the protein content or collagenase activity was observed in the deep pockets of the untreated Control Teeth in the same patients.

Membrane Components of Treponema denticola Trigger Proteinase Release from Human Polymorphonuclear Leukocytes

“…Conversely, the total amount of matrix metalloproteinases in the periodontium as well as the ratio of active to latent enzyme are decreased following periodontal therapy (Hakkarainen et al, 1988;McCulloch, 1994).…”

Relationship of collagenase and cathepsin G activity in gingival crevicular fluid

“…Collagenase activity in gingival crevicular fiuid has been shown to decrease in response to periodontal treatment also in previous studies (13)(14)(15) and it has been suggested to correlate primarily with pocket depth (14). In these studies, however, only total collagenase activity was monitored.…”

“…Interstitial collagenases are secreted from the cells into the extracellular space in a latent form which is subsequently activated by the disease process (12). Increased collagenase activities have been reported in inflamed human gingiva and gingival crevicular fluid in respect to healthy tissues and this increased collagenase activity has been shown to decrease in response to treatment procedures (13)(14)(15)(16). Only recently was it shown that the predominant collagenolytic activity in gingival crevicular fluid of patients with adult periodontitis is MMP-8 (10,(16)(17)(18)(19).…”

Occlusal trauma and excessive occlusal forces: Narrative review, case definitions, and diagnostic considerations

“…Occlusal adjustment is defined as “reshaping the occluding surfaces of teeth by grinding to create harmonious contact relationships between the maxillary and mandibular teeth.” The evidence linking occlusal adjustment to improvement in periodontal parameters is limited. In an earlier study, the flow rate and quality of gingival crevicular flow (GCF) after removal of occlusal interferences was examined in patients with advanced periodontitis . It was found that occlusal adjustment reduced the protein content and collagenase activity without affecting the quantity of GCF.…”