Collagen Extraction from Malaysian Cultured Catfish (Hybrid Clarias Sp.): Kinetics and Optimization of Extraction Conditions Using Response Surface Methodology

Abstract

A central composite design (CCD) was used for the experimental design and results analysis to obtain the optimal processing parameters (acetic acid concentration, liquid to solid ratio, and stirring speed) for the extraction of pepsin soluble collagen (PSC) from muscles of cultured hybrid catfish of Clarias sp. (Clarias gariepinus ×C. macrocephalus). Statistical analysis showed that the linear and quadratic terms of these three independent variables had significant effects on the yield of PSC. There was also an interaction between the ratio of liquid to solid and the stirring speed in affecting the extraction efficiency. Optimal conditions for a higher yield of PSC were an acetic acid concentration of 0.67 M, a liquid to solid ratio of 24.65 ml/g, and the stirring speed of 423.64 rpm. The verification of the optimization showed that the percentage error differences between the experimental and predicted values were in the range of 0.22-4.42%. The experimental values agreed with the predicted values, indicating an excellent fit of the model used and the success of the response surface methodology in modeling the extraction of PSC from the muscles of catfish. The experimental results were also fitted to the power law model and it was proven to be appropriate in describing the kinetics of collagen extraction process.

Optimizing the Extraction of Acid-soluble Collagen Inside the Eggshell Membrane

“…1). The results of this work confirmed previous reports by Kiew and Don (2012) and Wang et al (2008), who concluded that collagen can be extracted more efficiently from cultured catfish and grass carp by extending the treatment time and stirring speed. However, longer extraction durations require higher expenditures of energy.…”

“…A greater solubility of the eggshell membrane in acetic acid was observed, thereby exhibiting a higher potential for collagen's particles to be extracted from the eggshell membrane and to enter the medium. A further increase in the speed, making it faster than 400 rpm, however, failed to yield substantial improvements regarding extraction results (Kiew and Don, 2012). Extraction time and yield correlated positively (Figs.…”

Kinetic modeling of the alkaline deproteinization of Nile-tilapia skin for the production of collagen

“…The R 2 of Eq. (9) was 0.982, indicating a close agreement between the experimental results and values predicted by the statistical model (Kiew and Mat Don, 2012). The error analysis indicated that the coefficient of variation was 1.16%, confirming the validity of the model for the level of confidence employed (Gunst et al, 2006).…”

“…The SNT free of NCP, obtained under optimal conditions, was subjected to extraction with 0.7 M acetic acid (40 mLg -1 ), stirring at 70 rpm during 5 h ( (Kiew and Mat Don, 2012). The extraction was performed at 20 C because the properties of obtained collagen exhibited non-significant differences with those of the collagen obtained at lower temperatures (Potaros et al, 2009).…”

“…However, it is possible to compare our results with those obtained in the collagen extraction from Malaysian cultured Catfish (Hybrid Clarias Sp.) (Kiew and Mat Don, 2012), who used the same stirring speed in the acid extraction. Extraction times for first, second and third alkaline extractions were 5, 6 and 7 h, respectively, based on the initial slope of the curves in Figure 1B.…”