Collagen-Chitosan- Glycerol-HPMC Composite as Cornea Artificial Candidate

Abstract

The number of blindness is tend to be increased year by year. One of the blindness cause is cornea ulcer.The cause of cornea ulcer is bacteria, fungi, and herpes simplex virus. Cornea transplantation is the only treatment which could widely accepted for blindness. Transplant by donor network becomes the only treatment that is acceptable on a large for blindness. However, treatment donor transplants have many shortcomings in complications post surgery such as host response, donor limitations, incompatibility and the length of time healing. As technology develops, there are many corneal substitutes based on natural ingredients derived from collagen or their derivatives because they promise better properties in biocompatibility. The aim of research are to conduct the synthesis and characterization of collagen- chitosan- glycerol - HPMC as artificial cornea such functional cluster test, cytotoxycity test, morphological test and antibacterial test. Based on functional cluster test, there are functional groups of all components of composite materials. While from cytotoxicity test, all samples have a percentage of living cells above 85%. The morphology test is showed that the pore size of sample B with composition collagen-chitosan-glycerol-HPMC is in accordance with the standard pore size for keratoprothesis. Sample A (collagen-chitosan-glycerol) and sample B (collagen-chitosan-glycerol-HPMC) have strong antibacterial properties.Biocomposite of collagen-chitosan-glycerol could be considered as artificial cornea due to the proximity with the corneal characteristics.

Hydrogels as Emerging Materials for Cornea Wound Healing

“…Huh7it Heper Cells Show antibacterial properties. 2019 [150] Collagen-PCL corneal graft Human corneal epithelial cells Water absorption similar to native cornea. Facilitate cell adhesion, proliferation and migration.…”

The Effect of Collagen-Chitosan-Sodium Hyaluronates Intrastromal Implantation on Corneal Clarity and Transforming Growth Factor (TGF-ß) (Experimental Study On New Zealand Rabbit)

“…Chitosan is biocompatible, biodegradable, inert and non-toxic 7 . Chitosan also have anti-fungal, anti-bacterial, and hemostatic properties so it is suitable for use as a material for tissue engineering 29 . Sodium hyaluronate is a glycosaminoglycan in the extracellular matrix, which has a vital role in wound healing and inflammation.…”

“…In the eye plane, sodium hyaluronate has viscoelastic properties, so it is often used to protect the corneal endothelium and maintain the anterior chamber's depth during cataract surgery. Animal studies have also shown that sodium hyaluronate supports corneal epithelial wound healing by stimulating corneal epithelial migration, adhesion, and proliferation 8 .The addition of chitosan to collagen can improve its stability and structural integrity so that it can be implanted in the eye 9 and the addition of sodium hyaluronate improves light transmission 28,29 . This study attempted to evaluate the differences in Collagen-Chitosan-sodium hyaluronates (Col-Chi-NaHa) intrastromal implantation to the corneal haziness level TGF-ß expression in stromal injury on New Zealand rabbits eye.…”