Collagen biosynthesis in vitro and the consequences of bacterial contamination. Studies on vascular connective tissue in rabbits

Manthorpe, R

doi:10.1007/bf01946730

Cited by 2 publications

(2 citation statements)

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“…MANTHORPE (1973) found that bacteria in an incubation mixture with rabbit aorta greatly increased tlie incorporation of ^''C-proline into protein and hydroxy-proline, stressing the importance of sterile conditions. The teeth were split open with a tweezer, the pulps transferred to a plastic Petri dish on crushed ice, and sliced into pieces of about 1 mm'' with two scalpels.…”

Section: Methodsmentioning

confidence: 99%

Effect of fluoride on protein and collagen biosynthesis in rabbit dental pulp in vitro

Helgeland

1976

European J Oral Sciences

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– The time course for incorporation of 14C‐proline into various fractions of rabbit dental pulp in vitro has been measured. In the TCA‐soluble precursor pool a steady state level of activity was indicated upon incubation after 3 h, whereas incorporation into protien and 14C‐hydroxyproling, i.e. collagen formation, increased lineraly for 9 h, leaving off upon further incubation. A lag period of about 3 h was indicated for the appearance of high molecular weight 14C‐activity, including 14C‐hydroxyproline, in the medium, increasing linearly from 3 h to the end of the incubaition period (22 H). in this system, fluoride exhibited a dose‐dependent inhibitory effect. at 5.3 mM fluoride the uptake of 14C‐proline into the TCA‐soluble pool was inhibited by about 50%, and the incorporation into protein and the subsequent conversion to hydroxyproline by about 90 and 60%, respectively. Release of collogen, i.e. 14C‐hydrozyproline‐contaning material, seemed to be the process most sensitive to fluoride; it was inhibited by about 50% at the lowest concertration (1.3 mM) tested.

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Section: Methodsmentioning

confidence: 99%

Effect of fluoride on protein and collagen biosynthesis in rabbit dental pulp in vitro

Helgeland

1976

European J Oral Sciences

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“…The test-tubes were shaken in a water bath at 37°C for 12 h. At the end of the incubation the samples were tested for bacterial contamination (Manthorpe 1973). The intima-media was dissected free from the adventitial layer and cut into strips of 5-6 cm 0.8 mm.…”

Section: Biochemical Studiesmentioning

confidence: 99%

Effects of Glucocorticoid on Connective Tissue of Aorta and Skin in Rabbits

Manthorpe¹,

Helin²,

Kofod³

et al. 1974

Acta Endocrinologica

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Male rabbits were injected daily for 14 days with prednisone 2 mg/ kg. The content and metabolism of collagen and glycosaminoglycans, the content of RNA, DNA, fat and water and the 125I-albumin permeability were investigated in the skin and in the intima-media layer of the aorta. A saline injected group served as control. Since prednisone induced a decrease in body weight, a starvation group with a similar weight loss was included. Following injections of prednisone the protocollagen proline hydroxylase activity, 0.45 m NaCl soluble hydroxyproline, total hydroxyproline, dialysable 14C-hydroxyproline and non-dialysable 14C-hydroxyproline fractions were reduced in the skin. In the aorta, only the total 14C-hydroxyproline synthesis was decreased. Furthermore there was a decrease in 14C-proline incorporation and the alpha-amino nitrogen content in the skin as well as a decrease in the RNA content in the skin and the aorta.

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Collagen biosynthesis in vitro and the consequences of bacterial contamination. Studies on vascular connective tissue in rabbits

Cited by 2 publications

References 10 publications

Effect of fluoride on protein and collagen biosynthesis in rabbit dental pulp in vitro

Effect of fluoride on protein and collagen biosynthesis in rabbit dental pulp in vitro

Effects of Glucocorticoid on Connective Tissue of Aorta and Skin in Rabbits

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