Male rabbits were injected daily for 14 days with prednisone 2 mg/ kg. The content and metabolism of collagen and glycosaminoglycans, the content of RNA, DNA, fat and water and the 125I-albumin permeability were investigated in the skin and in the intima-media layer of the aorta. A saline injected group served as control. Since prednisone induced a decrease in body weight, a starvation group with a similar weight loss was included. Following injections of prednisone the protocollagen proline hydroxylase activity, 0.45 m NaCl soluble hydroxyproline, total hydroxyproline, dialysable 14C-hydroxyproline and non-dialysable 14C-hydroxyproline fractions were reduced in the skin. In the aorta, only the total 14C-hydroxyproline synthesis was decreased. Furthermore there was a decrease in 14C-proline incorporation and the alpha-amino nitrogen content in the skin as well as a decrease in the RNA content in the skin and the aorta.
The effect of ammonium chloride and sodium bicarbonate administration on urinary excretion of 14C‐imipramine in man and on tissue distribution of 14C‐imipramine in rats has been studied. Oral administration of ammonium chloride to man increased the total excretion of orally administered 14C‐imipramine about 10 to 40 per cent. This increase was due to a 50 to 100 per cent increase in excretion of nonconjugated metabolites. In particular the excretion of unchanged imipramine and desmethylimipramine was sensitive to changes in urinary pH. Compared to sodium chloride‐treated rats, in rats treated with ammonium chloride the uptake of 14C‐imipramine in brain was reduced some 15 per cent.
In a prospective 9 month study at a medical department with special interest in immunology and rheumatology, 10 patients with spuriously low thrombocyte counts ‐ pseudothrombocytopenia ‐ were registered.
The phenomenon was observed when the counting of platelets was performed on a Thrombocounter C apparatus or by manual counting using K2EDTA stabilized blood since platelet agglutination was considerably less pronounced when solutio citratis glucosi fortis (ACD) was used as the anticoagulant.
Results of our in vitro studies indicate that the mechanisms contributing to pseudothrombocytopenia involve the interacton of circulating immune complexes with platelet membrane Fc receptors causing agglutination. Fab'2‐fragments of isolated patient IgG did not react with normal donor thrombocytes. This finding argues against agglutination due to true antiplatelet antibody activity.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.