Cold Stress in Antarctic Fungi Targets Enzymes of the Glycolytic Pathway and Tricarboxylic Acid Cycle

Kostadinova, Nedelina; Vassilev, Spassen V.; Spasova, Boryana; Angelova, Maria

doi:10.5504/bbeq.2011.0122

Cited by 20 publications

(16 citation statements)

References 40 publications

(41 reference statements)

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“…However, its reverse reaction, which is catalyzed by fructose 1,6-bisphosphatase in the gluconeogenesis pathway, increased by 1.3-fold during the logarithmic phase. Another key enzyme in glycolysis, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), was overexpressed by 2.6-fold and 1.8-fold separately during the logarithmic phase and stationary phase, which was in agreement with Nedelina’s results 47 . Upregulation of GAPDH transcripts could be explained by its importance in stress conditions leading glycolytic flux deviation to accumulation of reserve carbohydrates 48 .…”

Section: Discussionsupporting

confidence: 89%

“…Nedelina et al . 47 found that short-term exposure to cold stress led to a general decrease in the activity of HK in Penicillium sp. and Aspergillus glaucus ; these authors attributed these results to the inhibition of HK by the accumulation of trehalose in the cytosol at low temperatures as a stress protectant.…”

Section: Discussionmentioning

confidence: 99%

“…The pentose phosphate pathway (PPP) is a biochemical pathway parallel to glycolysis that generates NADPH and pentose. Many studies have indicated that the key enzymes in the PPP pathway, glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (PGD), are overexpressed under different adverse conditions such as high-salinity stress 21 , cold stress 33 47 and oxidative stress 49 to maintain the balance of NADPH. Our results showed that under cold stress, the expression of G6PD only decreased by 1.2-fold during the logarithmic phase, whereas PGD expression increased by 1.3-fold during the stationary phase.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome and gene expression analysis of DHA producer Aurantiochytrium under low temperature conditions

Tan

Cui

et al. 2015

Sci Rep

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Aurantiochytrium is a promising docosahexaenoic acid (DHA) production candidate due to its fast growth rate and high proportions of lipid and DHA content. In this study, high-throughput RNA sequencing technology was employed to explore the acclimatization of this DHA producer under cold stress at the transcriptional level. The overall de novo assembly of the cDNA sequence data generated 29,783 unigenes, with an average length of 1,200 bp. In total, 13,245 unigenes were annotated in at least one database. A comparative genomic analysis between normal conditions and cold stress revealed that 2,013 genes were differentially expressed during the growth stage, while 2,071 genes were differentially expressed during the lipid accumulation stage. Further functional categorization and analyses showed some differentially expressed genes were involved in processes crucial to cold acclimation, such as signal transduction, cellular component biogenesis, and carbohydrate and lipid metabolism. A brief survey of the transcripts obtained in response to cold stress underlines the survival strategy of Aurantiochytrium; of these transcripts, many directly or indirectly influence the lipid composition. This is the first study to perform a transcriptomic analysis of the Aurantiochytrium under low temperature conditions. Our results will help to enhance DHA production by Aurantiochytrium in the future.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Transcriptome and gene expression analysis of DHA producer Aurantiochytrium under low temperature conditions

Tan

Cui

et al. 2015

Sci Rep

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“…In contrast, GPDH, the key enzyme in pentose phosphate pathway, showed notable increase at 10% NaCl concentration. These observations were also matched with findings of Kostadinova et al [12]. They reported that short-term exposure of Penicillium sp.…”

Section: Discussionsupporting

confidence: 91%

Deciphering the salinity adaptation mechanism in Penicilliopsis clavariiformis AP, a rare salt tolerant fungus from mangrove

Kashyap

Rai

Singh

et al. 2015

J. Basic Microbiol.

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Penicilliopsis clavariiformis AP, a rare salt tolerant fungus reported for the first time from India was identified through polyphasic taxonomy. Scanning electron microscopy showed that the fungus has unique features such as biverticillate penicilli bearing masses of oval to ellipsoidal conidia. The fungus has been characterized for salt tolerance and to understand the relevance of central carbon metabolism in salt stress adaptation. It showed optimal growth at 24 °C and able to tolerate up to 10% (w/v) NaCl. To understand the mechanism of adaptation to high salinity, activities of the key enzymes regulating glycolysis, pentose phosphate pathway, and tricarboxylic acid cycle were investigated under normal (0% NaCl) and saline stress environment (10% NaCl). The results revealed a re-routing of carbon metabolism away from glycolysis to the pentose phosphate pathway (PPP), served as a cellular stress-resistance mechanism in fungi under saline environment. The detection and significant expression of fungus genes (Hsp98, Hsp60, HTB, and RHO) under saline stress suggest that these halotolerance conferring genes from the fungus could have a role in fungus protection and adaptation under saline environment. Overall, the present findings indicate that the rearrangement of the metabolic fluxes distribution and stress related genes play an important role in cell survival and adaptation under saline environment.

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“…Fungal cells accelerated the production of glucose by gluconeogenesis and the production of amino acids important to compose the repertoire of molecules to the oxidative stress response (Dos Reis et al 2013). Kostadinova et al (2011) showed a re-routing of carbon metabolism away from glycolysis into the pentose phosphate pathway which serves as a cellular stress-resistance mechanism under cold stress conditions. Data published in the literature on the effect of low temperatures on glucose uptake have shown contradictory results.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the oxidative stress response of two Antarctic fungi to different growth temperatures

Kostadinova¹,

Tosi²,

Spassova³

et al. 2017

Polish Polar Research

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Two fungal strains, isolated from Livingston Island, Antarctica (Penicillium commune 161, psychrotolerant and Aspergillus glaucus 363, mesophilic) were investigated for a relationship between growth temperature and oxidative stress response. Cultivation at temperatures below -(10 and 15°C and 10 and 20°C for P. commune and A. glaucus, respectively) and above (25°C and 30°C for P. commune and A. glaucus, respectively) the optimum caused significant difference in growth and glucose uptake in comparison with the control cultures. Enhanced level of reserve carbohydrates (glycogen and trehalose) was determined under cultivation at different temperatures from the optimal one. While the highest content of trehalose was found in the exponential phase, glycogen accumulation was observed in the stationary phase when growth conditions deteriorate. The growth at temperature below-and above-optimum caused strain-dependent changes in two antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). While SOD activity in the psychrotolerant strain increases with decreasing of growth temperature, the mesophilic A. glaucus demonstrated marked reduction of it at below-and above-optimal temperature. Decreasing trend of CAT activity was observed in both strains below the optimal temperature indicating a lack of antioxidant protection from this enzyme under the cold stress conditions.

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Cold Stress in Antarctic Fungi Targets Enzymes of the Glycolytic Pathway and Tricarboxylic Acid Cycle

Cited by 20 publications

References 40 publications

Transcriptome and gene expression analysis of DHA producer Aurantiochytrium under low temperature conditions

Transcriptome and gene expression analysis of DHA producer Aurantiochytrium under low temperature conditions

Deciphering the salinity adaptation mechanism in Penicilliopsis clavariiformis AP, a rare salt tolerant fungus from mangrove

Comparison of the oxidative stress response of two Antarctic fungi to different growth temperatures

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