Comparison of the oxidative stress response of two Antarctic fungi to different growth temperatures

Kostadinova, Nedelina; Tosi, Solveig; Spassova, Boryana; Angelova, Maria

doi:10.1515/popore-2017-0015

Cited by 8 publications

(6 citation statements)

References 44 publications

(54 reference statements)

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“…Antarctica is isolated geographically from other continents and presents an extremely harsh environment on the earth. Antarctic environment has the characteristics of low temperature, high salinity, low nutrient availability, and strong ultraviolet (UV) radiation [1]. To adapt to the extreme conditions, microorganisms can produce a series of enzymes and display unique metabolic properties.…”

Section: Introductionmentioning

confidence: 99%

“…To adapt to the extreme conditions, microorganisms can produce a series of enzymes and display unique metabolic properties. Therefore, Antarctic microorganisms play a crucial part in the field of biomass conversion and have great potential applications, which have aroused more attention [1]. Sea ice is an important component of Antarctic climate and ecosystems.…”

Section: Introductionmentioning

confidence: 99%

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Cold-Adapted Glutathione S-Transferases from Antarctic Psychrophilic Bacterium Halomonas sp. ANT108: Heterologous Expression, Characterization, and Oxidative Resistance

et al. 2019

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Glutathione S-transferases are one of the most important antioxidant enzymes to protect against oxidative damage induced by reactive oxygen species. In this study, a novel gst gene, designated as hsgst, was derived from Antarctic sea ice bacterium Halomonas sp. ANT108 and expressed in Escherichia coli (E. coli) BL21. The hsgst gene was 603 bp in length and encoded a protein of 200 amino acids. Compared with the mesophilic EcGST, homology modeling indicated HsGST had some structural characteristics of cold-adapted enzymes, such as higher frequency of glycine residues, lower frequency of proline and arginine residues, and reduced electrostatic interactions, which might be in relation to the high catalytic efficiency at low temperature. The recombinant HsGST (rHsGST) was purified to apparent homogeneity with Ni-affinity chromatography and its biochemical properties were investigated. The specific activity of the purified rHsGST was 254.20 nmol/min/mg. The optimum temperature and pH of enzyme were 25 °C and 7.5, respectively. Most importantly, rHsGST retained 41.67% of its maximal activity at 0 °C. 2.0 M NaCl and 0.2% H2O2 had no effect on the enzyme activity. Moreover, rHsGST exhibited its protective effects against oxidative stresses in E. coli cells. Due to its high catalytic efficiency and oxidative resistance at low temperature, rHsGST may be a potential candidate as antioxidant in low temperature health foods.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cold-Adapted Glutathione S-Transferases from Antarctic Psychrophilic Bacterium Halomonas sp. ANT108: Heterologous Expression, Characterization, and Oxidative Resistance

et al. 2019

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“…Moreover, glycogen accumulation seems to be of primary importance for viability at 6°C. Such cold stress response was demonstrated by other fungi and yeasts (Aguilera et al 2007;Gocheva et al 2009;Kostadinova et al 2017). Some studies reported data linking glycogen to microbial viability, suggesting that it is involved in the stress response (Perez-Torrado et al 2002).…”

Section: Discussionmentioning

confidence: 90%

Polish Polar Research

Krumova,

Koeva,

Stoitsova

et al. 2021

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During the evolution organisms are subjected to the continuous impact of environmental factors. In recent years an increasing number of studies have focused on the physicochemical limits of life on Earth such as temperature, pressure, drought, salt content, pH, heavy metals, etc. Extreme environmental conditions disrupt the most important interactions that support the function and structure of biomolecules. For this reason, organisms inhabiting extreme habitats have recently become of particularly great interest. Although filamentous fungi are an important part of the polar ecosystem, information about their distribution and diversity, as well as their adaptation mechanisms, is insufficient. In the present study, the fungal strain Penicillium griseofulvum isolated from an Antarctic soil sample was used as a study model. The fungal cellular response against short term exposure to low temperature was observed. Our results clearly showed that short-term low temperature exposure caused oxidative stress in fungal cells and resulted in enhanced level of oxidative damaged proteins, accumulation of reserve carbohydrates and increased activity of the antioxidant enzyme defence. Ultrastructural changes in cell morphology were analysed. Different pattern of cell pathology provoked by the application of two stress temperatures was detected. Overall, this study aimed to observe the survival strategy of filamentous fungi in extremely cold habitats, and to acquire new knowledge about the relationship between low temperature and oxidative stress.

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“…In fact, the activation of mechanisms for protection against oxidative stress plays a crucial role in psychrophilic fungi. It has been shown that a cold environment induces the production of reactive oxygen species (ROS), which can cause severe damage to DNA, lipids and proteins, affecting the cell survival (Kostadinova et al ., 2017). The expansion of genes for Zn(2)-C6 transcription factors in C. antarcticus and other psychrophilic extremophiles may help to counter this damage.…”

Section: Discussionmentioning

confidence: 99%

Comparative genomics of the extremophileCryomyces antarcticusand other psychrophilic Dothideomycetes

Gomez,

Sic,

Haridas

et al. 2024

Preprint

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Cryomyces antarcticusis an endolithic fungus that inhabits rock outcrops in Antarctica. It survives extremes of cold, humidity and solar radiation in one of the least habitable environments on Earth. This fungus is unusual because it produces heavily melanized, meristematic growth and is thought to be haploid and asexual. Due to its growth in the most extreme environment, it has been suggested as an organism that could survive on Mars. However, the mechanisms it uses to achieve its extremophilic nature are not known. Over a billion years of fungal evolution has enabled representatives of this kingdom to populate almost all parts of planet Earth and to adapt to some of its most uninhabitable environments including extremes of temperature, salinity, pH, water, light, or other sources of radiation. Comparative genomics can provide clues to the processes underlying biological diversity, evolution, and adaptation. This effort has been greatly facilitated by the 1000 Fungal Genomes project and the JGI MycoCosm portal where sequenced genomes have been assembled into phylogenetic and ecological groups representing different projects, lifestyles, ecologies, and evolutionary histories. Comparative genomics within and between these groups provides insights into fungal adaptations, for example to extreme environmental conditions. Here, we analyze two Cryomyces genomes in the context of additional psychrophilic fungi, as well as non-psychrophilic fungi with diverse lifestyles selected from the MycoCosm database. This analysis identifies families of genes that are expanded and contracted in Cryomyces and other psychrophiles and may explain their extremophilic lifestyle. Higher GC contents of genes and of bases in the third positions of codons may help to stabilize DNA under extreme conditions. Numerous smaller contigs in C. antarcticus suggest the presence of an alternative haplotype that could indicate that the sequenced isolate is diploid or dikaryotic. These analyses provide a first step to unraveling the secrets of the extreme lifestyle ofC. antarcticus.

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Comparison of the oxidative stress response of two Antarctic fungi to different growth temperatures

Cited by 8 publications

References 44 publications

Cold-Adapted Glutathione S-Transferases from Antarctic Psychrophilic Bacterium Halomonas sp. ANT108: Heterologous Expression, Characterization, and Oxidative Resistance

Cold-Adapted Glutathione S-Transferases from Antarctic Psychrophilic Bacterium Halomonas sp. ANT108: Heterologous Expression, Characterization, and Oxidative Resistance

Polish Polar Research

Comparative genomics of the extremophileCryomyces antarcticusand other psychrophilic Dothideomycetes

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