Cold storage of shoot cultures and alginate encapsulation of shoot tips of Camellia japonica L. and Camellia reticulata Lindley

Ballester, A.; Janeiro, Laura V.; Viéitez, A. M.

doi:10.1016/s0304-4238(97)00074-5

Cited by 56 publications

(23 citation statements)

References 22 publications

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“…Sucrose as a carbon source for encapsulated explants enables their survival during storage. The presence of sucrose in the alginate gel matrix also improved emergence of shoots from encapsulated shoot tips of Camelia japonica (Ballester et al, 1997) and axillary buds of Betula pendula (Piccioni and Standardi, 1995). Rai et al (2008) demonstrated complete inhibition of shoot development from encapsulated shoot tips of Psidium guajava in medium without sucrose.…”

Section: Resultsmentioning

confidence: 99%

A Protocol For Synthetic Seeds From Salvia Officinalis L. Shoot Tips

Grzegorczyk¹,

Wysokińska²

2011

Acta Biologica Cracoviensia Series Botanica

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Shoot tips excised from shoot culture of Salvia officinalis were encapsulated in 2% or 3% (w/v) sodium alginate and exposed to 50 mM calcium chloride for complexation. Immediately or after 6, 12 or 24 weeks of storage at 4°C, the synthetic seeds were cultured for 6 weeks on half-strength MS medium supplemented with indole-3-acetic acid (IAA) (0.1 mg/l) and solidified with 0.7% agar. The frequency of shoot and root emergence from encapsulated shoot tips was affected by the concentrations of sodium alginate and additives in the gel matrix (sucrose, gibberellic acid, MS nutrient medium) as well as duration of storage. The frequency of shoot and root induction of non-stored synthetic seeds was highest with shoot tips encapsulated with 2% sodium alginate containing 1.5% sucrose and 0.5 mg/l gibberellic acid (GA 3 ). Shoot tips maintained their viability and ability to develop shoots even after 24 weeks of storage when they were encapsulated in 3% alginate with 1/3 MS medium, sucrose (1.5%) and GA 3 (0.25 mg/l). Root formation tended to decrease with storage time. Overall, 90% of the plantlets derived from stored and non-stored synthetic seeds survived in the greenhouse and grew to phenotypically normal plants. This procedure can enable the use of synthetic seed technology for germplasm conservation of S. officinalis, a plant species of high medical and commercial value.K Ke ey y w wo or rd ds s: : Calcium alginate, cold storage, Salvia officinalis L., shoot tip encapsulation, synthetic seeds.

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Section: Resultsmentioning

confidence: 99%

A Protocol For Synthetic Seeds From Salvia Officinalis L. Shoot Tips

Grzegorczyk¹,

Wysokińska²

2011

Acta Biologica Cracoviensia Series Botanica

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“…Artificial seed would provide an easy and novel propagation system for the elite as well as difficult to root species (Bapat et al 1987, Ballester et al 1997. Also, encapsulation of propagules that were produced in vitro could reduce the cost of micropropagation of plantlets for commercialization and final delivery (Chu 1995, Nieves et al 2003.…”

Section: Introductionmentioning

confidence: 99%

In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

Islam

Bari

2014

J bio-sci.

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Context: The application of encapsulated shoot tips and nodal segments may contribute to the protection of rare and threatened medicinal plants. Although the artificial seed technique has been reported for more than two decades, for medicinal plants this method has not been developed sufficiently. The main limitations in conventional propagation of some species with medicinal value are: reduced endosperm, low germination rate and seedless varieties. The above mentioned reasons indicate the need for the production of artificial seeds as a technique which combines the advantages of clonal multiplication with those of seed propagation and storage. Objectives: The objective of the present investigation was to standardize artificial seed production technology taking shoot tip and nodal explants in Mentha arvensis and its in vitro regeneration Materials and Methods: Sodium alginate beads were produced by encapsulation of shoot tip and nodal segments of the plant M. arvensis. MS medium was used as basal medium with agar and sodium alginate was used as gelling agent accompanied by CaCl2 solution. Results: Different concentrations and combinations of BAP, Kin and NAA were used in alginate bead in MS basal medium. Among the different concentrations of phytohormone, highest 80% of shoot formation was observed in MS medium containing 2.0 mg/l BAP + 0.2 mg/l NAA from nodal segments of M. arvensis. Highest average number of shoot 9.87 ± 0.58 formation was obtained in the same medium but highest length of shoot 6.27 ± 0.29 cm was found in the medium having 1.0 mg/l BAP + 0.5 mg/l NAA. Conclusion:The present investigation clearly established and demonstrated the method of obtaining the artificial seed production in M. arvensis supported by different hormone concentrations

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“…Somatic embryogenesis is a process having several advantages over organogenesis, such as the probable single cell origin, which reduces somaclonal variations in several geophytes and is applicable for medium-or long-term storage in sodium alginate beads (Ballester et al, 1997;Patel et al, 2000;Gurel and Gurel, 2014). Heavy collection of bulbs from their natural habitats and their use for breeding propagation purposes could have negative impacts on the plant species.…”

Section: Discussionmentioning

confidence: 99%

Somatic embryogenesis and encapsulation of immature bulblets of an ornamental species, grape hyacinths (Muscari armeniacum Leichtlin ex Baker)

Yücesan¹,

Çiçek²,

Gürel³

2014

Turk J Agric For

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An efficient in vitro regeneration system was developed for the ornamental Muscari armeniacum on Linsmaier and Skoog basal medium (LS) supplemented with benzyladenine (BA) at varying concentrations (0.5, 1.0, or 2.0 mg/L) alone or in combination with 0.5 mg/L α-naphthalene acetic acid (NAA). The highest mean number of direct somatic embryo formations was observed on LS medium containing 2.0 mg/L BA and 0.5 mg/L NAA, with a mean of 7.9 somatic embryos per explant after 10 weeks of culture. Green nodular calli induced on LS medium containing 5.0 mg/L BA alone or in combination with 0.5 mg/L NAA were transferred to LS medium supplemented with or without 0.5 mg/L gibberellic acid (GA3) for 8 weeks, producing 23.3 immature bulblets. Immature bulblets produced in vitro were either embedded in a sodium alginate matrix for the encapsulation process or were transferred directly to LS medium supplemented with or without GA 3 at 0.5 or 1.0 mg/L for growth and development for 6 weeks. Encapsulated bulblets were then stored at 4 °C in darkness for 10 weeks and almost all encapsulated bulblets retained their viability and resumed their growth under nonaxenic greenhouse conditions.

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Cold storage of shoot cultures and alginate encapsulation of shoot tips of Camellia japonica L. and Camellia reticulata Lindley

Cited by 56 publications

References 22 publications

A Protocol For Synthetic Seeds From Salvia Officinalis L. Shoot Tips

A Protocol For Synthetic Seeds From Salvia Officinalis L. Shoot Tips

In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

Somatic embryogenesis and encapsulation of immature bulblets of an ornamental species, grape hyacinths (Muscari armeniacum Leichtlin ex Baker)

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