Cohaesibacter marisflavi sp. nov., isolated from sediment of a seawater pond used for sea cucumber culture, and emended description of the genus Cohaesibacter

Qu, Lingyun; Lai, Qiliang; Zhu, Fengling; Hong, Xiao; Sun, Xiaoqin; Shao, Zongze

doi:10.1099/ijs.0.021972-0

Cited by 22 publications

(8 citation statements)

References 13 publications

(21 reference statements)

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“…Phylogenetic analysis placed those two similar sequences (ASV226 and ASV11394, Fig. 8) with Cohaesibacter marisflavi, a species not currently known to be a pathogen (Qu et al, 2011). Although no Cohaesibacter species are known pathogens, Cohaesibacter intestini was isolated from the intestine of an invertebrate, abalone (Liu et al, 2019).…”

Section: Sctld Lesion Microbial Communities Are Unique From Healthy Tmentioning

confidence: 94%

Stony Coral Tissue Loss Disease biomarker bacteria identified in corals and overlying waters using a rapid field-based sequencing approach

Becker

Brandt

Miller

et al. 2021

Preprint

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Stony Coral Tissue Loss Disease (SCTLD) is a devastating disease. Since 2014, it has spread along the entire Florida Reef Tract, presumably via a water-borne vector, and into the greater Caribbean. It was first detected in the United States Virgin Islands (USVI) in January 2019. To more quickly identify disease biomarker microbes, we developed a rapid pipeline for microbiome sequencing. Over a span of 10 days we collected, processed, and sequenced coral tissue and near-coral seawater microbiomes from diseased and apparently healthy Colpophyllia natans, Montastraea cavernosa, Meandrina meandrites and Orbicella franksi. Analysis of the resulting bacterial and archaeal 16S ribosomal RNA sequences revealed 25 biomarker amplicon sequence variants (ASVs) enriched in diseased tissue. These biomarker ASVs were additionally recovered in near-coral seawater (within 5 cm of coral surface), a potential recruitment zone for pathogens. Phylogenetic analysis of the biomarker ASVs belonging to Vibrio, Arcobacter, Rhizobiaceae, and Rhodobacteraceae revealed relatedness to other coral disease-associated bacteria and lineages novel to corals. Additionally, four ASVs (Algicola, Cohaesibacter, Thalassobius and Vibrio) were exact sequence matches to microbes previously associated with SCTLD. This work represents the first rapid coral disease sequencing effort and identifies biomarkers of SCTLD that could be targets for future SCTLD research.

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Section: Sctld Lesion Microbial Communities Are Unique From Healthy Tmentioning

confidence: 94%

Stony Coral Tissue Loss Disease biomarker bacteria identified in corals and overlying waters using a rapid field-based sequencing approach

Becker

Brandt

Miller

et al. 2021

Preprint

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“…(GQ200200)Rhizobiales; Cohaesibacteraceae. Isolated from sediment of a seawater pond used for sea cucumber cultureQu et al, 2011P.o_rhiz_23b (JF292434)Rhizome/Talamanca51892Uncultured δ-proteobacteria (AY133092)Isolated from TCE-contaminated siteCarrol and Zinder, Unpublished Desulfovibrionaceae /δ-proteobacteria92Uncultured bacterium clone (GU118736)Isolated from the coral Montastraea franksi Sunagawa et al, 201091Bacterium enrichment culture clone (HQ622261)Polluted estuarine sedimentAbed et al, Unpublished91 Desulfarculus baarsii (CP002085)Desulfarculales; Desulfarculaceae; DesulfarculusLucas et al, UnpublishedP.o_root_15c (JF292435)Root/C.Marmacen47196 Roseovarius sp. (HQ871860 + HQ871851)Rhodobacterales; Rhodobacteraceae; RoseovariusJeanthon et al, Unpublished Rhodobacteraceae /α-proteobacteria96 Pelagibaca sp.…”

Section: Table A1mentioning

confidence: 99%

Endophytic bacterial community of a Mediterranean marine angiosperm (Posidonia oceanica)

Garcías-Bonet¹,

Arrieta²,

Santana³

et al. 2012

Front. Microbio.

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Bacterial endophytes are crucial for the survival of many terrestrial plants, but little is known about the presence and importance of bacterial endophytes of marine plants. We conducted a survey of the endophytic bacterial community of the long-living Mediterranean marine angiosperm Posidonia oceanica in surface-sterilized tissues (roots, rhizomes, and leaves) by Denaturing Gradient Gel Electrophoresis (DGGE). A total of 26 Posidonia oceanica meadows around the Balearic Islands were sampled, and the band patterns obtained for each meadow were compared for the three sampled tissues. Endophytic bacterial sequences were detected in most of the samples analyzed. A total of 34 OTUs (Operational Taxonomic Units) were detected. The main OTUs of endophytic bacteria present in P. oceanica tissues belonged primarily to Proteobacteria (α, γ, and δ subclasses) and Bacteroidetes. The OTUs found in roots significantly differed from those of rhizomes and leaves. Moreover, some OTUs were found to be associated to each type of tissue. Bipartite network analysis revealed differences in the bacterial endophyte communities present on different islands. The results of this study provide a pioneering step toward the characterization of the endophytic bacterial community associated with tissues of a marine angiosperm and reveal the presence of bacterial endophytes that differed among locations and tissue types.

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“…The sediment sample was collected in March 2009. Strains DQHS4 T , DQHS8 and DQHS15 were isolated and subsequently purified five times on 2216E agar medium (per litre Yellow Sea seawater: 5 g tryptone, 1 g yeast extract, 15 g agar, pH 7.5) at 25 °C as described previously (Qu et al , 2011). The strains were maintained on 2216E agar medium at 30 °C and stored at −80 °C in 2216E medium supplemented with 30 % (v/v) glycerol.…”

mentioning

confidence: 99%

Sunxiuqinia elliptica gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from sediment in a sea cucumber farm

Zhu

Hong

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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Sunxiuqinia elliptica gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from sediment in a sea cucumber farm Three novel aerobic, elliptic bacteria, designated DQHS4 T , DQHS8 and DQHS15, were isolated from sediment of a seashore pond for sea cucumber culture in Jimo, Qingdao, on the east coast of China. Cells were Gram-, oxidase-and catalase-negative. All three strains grew at 15-42 6C, pH 5-9 and NaCl concentrations between 0.5 and 10 %. DNA-DNA hybridization experiments revealed high (.85 %) relatedness among the three novel isolates and suggested that the strains constitute a single species. Comparative 16S rRNA gene sequence analysis indicated that these bacteria had less than 90 % similarity to all described species of the phylum Bacteroidetes; the closest relative of the three isolates was Prolixibacter bellariivorans F2 T , sharing only 89.6 % sequence similarity. The major cellular fatty acids were iso-C 17 : 0 3-OH (19.8-20.0 %), iso-C 15 : 0 (16.9-17.3 %), anteiso-C 17 : 1 B and/or iso-C 17 : 1 I (7.4-8.7 %), C 17 : 0 2-OH (8.4 %), anteiso-C 15 : 0 (8.2-8.6 %) and C 17 : 1 v6c (5.6-6.0 %). The major respiratory quinone was menaquinone-7 (MK-7) and the DNA G+C content was 41.8-43.5 mol%. Based on the distinct phylogenetic position and the combination of genotypic, phenotypic and chemotaxonomic characteristics, these three strains were considered to represent a novel species of a new genus in the phylum Bacteroidetes, for which the name Sunxiuqinia elliptica gen. nov., sp. nov. is proposed. The type strain of Sunxiuqinia elliptica is DQHS4 T (5CGMCC 1.9156 T 5NCCB 100301 T 5LMG 25367 T ).

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Cohaesibacter marisflavi sp. nov., isolated from sediment of a seawater pond used for sea cucumber culture, and emended description of the genus Cohaesibacter

Cited by 22 publications

References 13 publications

Stony Coral Tissue Loss Disease biomarker bacteria identified in corals and overlying waters using a rapid field-based sequencing approach

Stony Coral Tissue Loss Disease biomarker bacteria identified in corals and overlying waters using a rapid field-based sequencing approach

Endophytic bacterial community of a Mediterranean marine angiosperm (Posidonia oceanica)

Sunxiuqinia elliptica gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from sediment in a sea cucumber farm

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