Cognate Hsp70 gene is induced during deep larval diapause in the moth Sesamia nonagrioides

Abstract

The complete cDNA sequences of Heat shock cognate protein 70 (SnoHsc70) and Heat shock protein 70 (SnoHsp70) were determined from the corn stalk borer Sesamia nonagrioides (Lef.). They encode 653 amino acids (Hsc70) and 633 amino acids (Hsp70), with calculated molecular masses of 71.5 kDa and 70.2 kDa respectively. SnoHsc70 is constitutively expressed, and SnoHsp70 is heat-inducible in non-diapausing insects. SnoHsp70 is down regulated during diapause, while SnoHsc70 is induced as the larvae enter deep diapause. High temperature stress during diapause has no further effect on transcript levels of SnoHsc70. Our results show that SnoHsc70 may play important roles in assisting protein conformation during specific stages of diapause.

Gene expression, metabolic regulation and stress tolerance during diapause

“…The amount of SnoHsp70 mRNA dips as S. nonagrioides enters diapause, whereas the cognate SnoHsc70 mRNA multiplies in deep diapause [112], as does mRNA encoded by the Hsp83 gene, a homologue of Hsp90 [113]. These observations suggest diapause-related roles for Hsc70 and Hsp90, perhaps in maintaining protein structure and contributing to ecdysone action.…”

“…), an organism that feeds and moults throughout the period of reduced activity associated with diapause. SnoHsp19.5 mRNA remains constant for the duration of diapause, whereas SnoHsp20.8 mRNA falls off in middiapause and expands upon termination, suggesting [111] sHSP Down [111] Hsc70 Up [112] Hsp70 Down [112] Hsp90…”

Heat shock protein expression during stress and diapause in the marine copepod Calanus finmarchicus

“…For example, while changes in Hsp70 expression are not a component of the larval diapause of the blowfly (Tachibana et al, 2005), the pupal diapause of the corn earworm (Zhang and Denlinger, 2009) or the adult diapause of a fruit fly (Goto et al, 1998), Hsp70 is highly induced in the pupal diapause of the solitary bee (Yocum et al, 2005) and the onion maggot (Chen et al, 2006). Conversely, Hsp70 is down-regulated in the larval diapause of the corn stalk borer (Gkouvitsas et al, 2009) and the bamboo borer (Tungjitwitayakul et al, 2008). Hsp90 demonstrates similar discrepancies in diapause-related expression patterns, including down-regulation in the pupal diapause of the flesh fly , up-regulation at the termination of the 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 17 larval diapause of the blow fly (Tachibana et al, 2005), and constant expression in the pupal diapause of the solitary bee (Yocum et al, 2005).…”

Artemin, a Diapause-Specific Chaperone, Contributes to the Stress Tolerance of Artemia Cysts and Influences Their Release from Females

“…Diapause is usually limited to one life stage per organism which displays varying degrees of metabolic suppression and stress tolerance, occurring in embryos of the silkworm, Bombyx mori (Moribe et al, 2010), larvae of the moth Sesamia nonagrioides (Gkouvitsas et al, 2009a;Gkouvitsas et al, 2009b), pupae of the flesh fly Sarcophaga crassipalpis (Li et al, 2007) and adults of the mosquito Culex pipiens (Kim et al, 2010).…”