Heat shock protein expression during stress and diapause in the marine copepod Calanus finmarchicus

Aruda, Amalia; Baumgartner, Mark F.; Reitzel, Adam M.; Tarrant, Ann M.

doi:10.1016/j.jinsphys.2011.03.007

Cited by 82 publications

(74 citation statements)

References 64 publications

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“…High heavy metal concentrations are known to affect the rotifer community (Cecchine and Snell 1999;Weisse et al 2013). Additionally, the diapause pattern observed in different organisms as response to arsenic might be a general response to stressors (Aruda et al 2011). Nevertheless, a full assessment of fitness including the performance of individuals hatched from surviving eggs would be required to get further insight on this question.…”

Section: Discussionmentioning

confidence: 99%

Diapause as escape strategy to exposure to toxicants: response of Brachionus calyciforus to arsenic

Aránguiz-Acuña

Serra

2016

Ecotoxicology

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Invertebrate organisms commonly respond to environmental fluctuation by entering diapause. Production of diapause in monogonont rotifers involves a previous switch from asexual to partial sexual reproduction. Although zooplankton have been used in ecotoxicological assays, often their true vulnerability to toxicants is underestimated by not incorporating the sexual phase. We experimentally analyzed traits involved in sexual reproduction and diapause in the cyclically parthenogenetic freshwater rotifer, Brachionus calyciflorus, exposed to arsenic, a metalloid naturally found in high concentrations in desert zones, focusing on the effectiveness of diapause as an escape response in the face of an adverse condition. Addition of sublethal concentrations of arsenic modified the pattern of diapause observed in the rotifer: investment in diapause with arsenic addition peaked earlier and higher than in non-toxicant conditions, which suggests that sexual investment could be enhanced in highly stressed environmental conditions by increased responsiveness to stimulation. Nevertheless, eggs produced in large amount with arsenic, were mostly low quality, and healthy-looking eggs had lower hatching success, therefore it is unclear whether this pattern is optimum in an environment with arsenic, or if rather arsenic presence in water bodies disturbs the optimal allocation of offspring entering diapause. We observed high accumulation of arsenic in organisms exposed to constant concentration after several generations, which suggests that arsenic may be accumulated transgenerationally. The sexual phase in rotifers may be more sensitive to environmental conditions than the asexual one, therefore diapause attributes should be considered in ecotoxicological assessment because of its ecological and evolutionary implications on lakes biodiversity.

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Section: Discussionmentioning

confidence: 99%

Diapause as escape strategy to exposure to toxicants: response of Brachionus calyciforus to arsenic

Aránguiz-Acuña

Serra

2016

Ecotoxicology

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“…Aruda et al (2011) found that the heat shock proteins, hsp70a, hsp21, and hsp22, had significantly higher expression 3 h after handling with a plankton net, but did not find any significant differences 2 h after exposure. Rahlff et al (2017) found significant changes in expression of hsp70 in response to handling, which was reduced to negligible levels after 24 h. These prior studies suggest that the expression of hsp70 peaks within 24 h after stress exposure, and may explain why we did not observe an increase in expression level of this gene 24 h after handling stress.…”

Section: Discussionmentioning

confidence: 76%

“…Experimentally-induced and handling-related stress has been extensively studied in larger crustaceans (Fotedar and Evans, 2011). To our knowledge, only a few studies, e.g., Aruda et al (2011) and Rahlff et al (2017), have examined handling stress in copepods with targeted methods, which entail the evaluation of specific transcriptional biomarkers selected to evaluate certain stressors, typically by real-time quantitative PCR.…”

Section: Introductionmentioning

confidence: 99%

Environmental Stress Responses and Experimental Handling Artifacts of a Model Organism, the Copepod Acartia tonsa (Dana)

et al. 2018

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Handling animals during experiments potentially affects the differential expression of genes chosen as biomarkers of sub-lethal stress. RNA sequencing was used to examine whole-transcriptome responses caused by laboratory handling of the calanoid copepod, Acartia tonsa. Salinity shock (S = 35 to S = 5) was used as positive stress control; individuals not exposed to handling or other stressors served as negative stress control. All copepods were grown from eggs to adults without being handled or exposed to any stressors prior the experiment. Survival of nauplii and adults was estimated for up to 10 min of exposure to handling stress and salinity shock. Only adults exhibited decreased survival (44 ± 7% with 10 min of exposure) in response to handling stress and were selected for definitive experiments for RNA sequencing. After 10 min of experimental exposures to handling stress or salinity shock, adults were incubated for 15 min or 24 h at normal culture conditions. A small number of significantly differentially expressed genes (DEGs) were observed 15 min after exposure to handling stress (2 DEGs) or salinity shock (7 DEGs). However, 24 h after exposure, handling stress resulted in 276 DEGs and salinity shock resulted in 573 DEGs, of which 174 DEGs were overlapping between the treatments. Among the DEGs observed 24 h after exposure to handling stress or salinity shock, some commonly-used stress biomarkers appeared at low levels. This suggests that a stress-response was induced at the transcriptional level for these genes between 15 min and 24 h following exposure. Since handling stress clearly affects transcriptional patterns, it is important to consider handling when designing experiments, by either including additional controls or avoiding focus on impacted genes. Not considering handling in gene expression studies can lead to inaccurate conclusions. The present study provides a baseline for studying handling stress in future studies using this model organism and others.

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“…Constitutive expression of these hsps could be explained by multiple homologues within hsp families in Calanus spp. and their differential regulation under various stresses (Aruda et al, 2011;Smolina et al, 2015;Voznesensky et al, 2004). Therefore, additional whole transcriptome profiling would be beneficial and may result in detection of both populationspecific and population-universal molecular processes in response to stress.…”

Section: Discussionmentioning

confidence: 99%

Reduced up-regulation of gene expression in response to elevated temperatures in the mid-Atlantic population of Calanus finmarchicus

Smolina

Harmer

Lindeque

et al. 2016

Journal of Experimental Marine Biology and Ecology

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Heat shock protein expression during stress and diapause in the marine copepod Calanus finmarchicus

Cited by 82 publications

References 64 publications

Diapause as escape strategy to exposure to toxicants: response of Brachionus calyciforus to arsenic

Diapause as escape strategy to exposure to toxicants: response of Brachionus calyciforus to arsenic

Environmental Stress Responses and Experimental Handling Artifacts of a Model Organism, the Copepod Acartia tonsa (Dana)

Reduced up-regulation of gene expression in response to elevated temperatures in the mid-Atlantic population of Calanus finmarchicus

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