Coat protein deletion mutation of Mungbean yellow mosaic India virus (MYMIV)

Abstract

Mungbean yellow mosaic India virus (MYMIV) causing yellow mosaic disease in grain legumes belongs to the genus Begomovirus of the family Geminiviridae. The virus has a bipartite genome comprising two circular single stranded DNA components (DNA A and DNA B) and is transmitted by the whitefly, Bemisia tabaci Genn. DNA A encodes for coat protein on the viral strand and for replication associated proteins and transcription activator proteins in the complementary strand. The coat protein plays key role in whitefly transmission. In the present communication, the regions in coat protein that govern whitefly transmission and systemic spread were mapped by constructing deletion mutations. Results showed that inoculation with viral constructs having N′ terminal deletion of 75 and 150 amino acids affected systemic spread and pathogenicity in cowpea, mungbean and blackgram plants contrasting to French bean which developed symptoms similar to wild type. Assembly of particles and whitefly transmission were not seen in all the mutations. However there was minimum, six folds increase in viral DNA levels in French bean plants inoculated with N′ terminal deletions.

Spectral reflectance pattern in soybean for assessing yellow mosaic disease

“…JS 335 showing typical YMD symptoms was used as a source of inoculation. Presence of MYMIV was confirmed by polymerase chain reaction (PCR) using specific primer (AVI forward: 5 0 -GCATGATATTGCCCACACAG-3 0 and reverse: 5 0 -TCACGCAGATCGTTCTTCAC-3 0 ) to coat protein (AV1) gene of MYMIV [10,18,37]. Whitefly (Bemisia tabaci) culture and inoculation standard protocol [31] was followed.…”

“…In the Indian subcontinent, soybean production is seriously affected due to yellow mosaic disease (YMD). Two begomovirus species known to cause YMD of soybean are Mungbean yellow mosaic India virus (MYMIV) and Mungbean yellow mosaic virus [10,18,36]. The affected plants initially develop yellow specks followed by bright yellow mosaic on leaves.…”

Yellow Mosaic Disease (YMD) of Mungbean (Vigna radiata (L.) Wilczek): Current Status and Management Opportunities

“…However, when co-inoculation was performed with the Anti-Rep construct, both symptom severity and infection percentage become negligible. The deletion in the CP amino-acids at N0 (75 and 150) of MYMIV has found affecting both systemic spread and pathogenicity ( Haq et al, 2011 ), while agro-inoculation of the CP hairpin construct (Cphp) reported preventing the viral pathogenesis in mungbean ( Kumari and Malathi, 2012 ). Kumar et al (2017b) demonstrated RNAi-derived resistance to MYMIV in cowpea, where agro-infection of transgenic lines expressing AC2- hp and AC2 + AC4-hp RNA showed nearly absolute resistance.…”

Mungbean yellow mosaic virus (MYMV): a threat to green gram (Vigna radiata) production in Asia

“…CP deletion (N 0 terminal deletion of 75 and 150 amino acids) showed the mutation of MYMIV. It affected the systemic spread and pathogenicity in mungbean (Haq et al 2011b). The hairpin constructs of CP has 35s promoter (1.3 kb) with the sense sequence of MYMIV (130 bp), followed by an intron (741 bp), after which the antisense (130 bp) target sequence with the OCS terminator (765 bp) are placed and cloned in a cloning vector called Phannibal.…”