Yellow Mosaic Disease (YMD) of Mungbean (Vigna radiata (L.) Wilczek): Current Status and Management Opportunities

Mishra, Gyan P.; Dikshit, Harsh Kumar; Ramesh, S. V.; Tripathi, Kuldeep; Kumar, Ranjeet; Aski, Muraleedhar S.; Singh, Akanksha; Roy, Anirban; Priti,; Kumari, Nikki; Dasgupta, Uttarayan; Kumar, Atul; Praveen, Shelly; Nair, Ramakrishnan M.

doi:10.3389/fpls.2020.00918

Cited by 46 publications

(23 citation statements)

References 155 publications

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“…Mungbean is indigenous to India or Indo-Burma region, as the Central Asian region harbors the abundance of various cultivated and wild species and considered as the ‘primary center’ of genetic diversity [ 2 ]. Across Asia, mungbean is consumed as a cheap source of protein predominantly in developing countries, thus playing a remarkable role in the alleviation of protein malnutrition [ 3 , 4 ]. Mungbean protein and carbohydrate are easily digestible and create less flatulence compared to other legumes and is also a good source of minerals like iron (40–70 ppm) [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…Across Asia, mungbean is consumed as a cheap source of protein predominantly in developing countries, thus playing a remarkable role in the alleviation of protein malnutrition [ 3 , 4 ]. Mungbean protein and carbohydrate are easily digestible and create less flatulence compared to other legumes and is also a good source of minerals like iron (40–70 ppm) [ 3 , 4 ]. Furthermore, the relatively smaller genome size (579 mbp) also makes it like a model crop for high throughput genomic assisted breeding [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…Both these species contain a bipartite genome and have a very narrow host range within the legumes. MYMV is most prevalent in Western and Southern India, Thailand and Indonesia, while MYMIV is present in Central, Eastern and Northern India, Pakistan, Bangladesh, Nepal and Vietnam [ 3 , 12 ]. Recombination between these two species though does not occur frequently but some reports indicated the occurrence of recombinant viruses in northern India [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Comparative RNA-Seq analysis unfolds a complex regulatory network imparting yellow mosaic disease resistance in mungbean [Vigna radiata (L.) R. Wilczek]

et al. 2021

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Yellow Mosaic Disease (YMD) in mungbean [Vigna radiata (L.) R. Wilczek] is one of the most damaging diseases in Asia. In the northern part of India, the YMD is caused by Mungbean Yellow Mosaic India Virus (MYMIV), while in southern India this is caused by Mungbean Yellow Mosaic Virus (MYMV). The molecular mechanism of YMD resistance in mungbean remains largely unknown. In this study, RNA-seq analysis was conducted between a resistant (PMR-1) and a susceptible (Pusa Vishal) mungbean genotype under infected and control conditions to understand the regulatory network operating between mungbean-YMV. Overall, 76.8 million raw reads could be generated in different treatment combinations, while mapping rate per library to the reference genome varied from 86.78% to 93.35%. The resistance to MYMIV showed a very complicated gene network, which begins with the production of general PAMPs (pathogen-associated molecular patterns), then activation of various signaling cascades like kinases, jasmonic acid (JA) and brassinosteroid (BR), and finally the expression of specific genes (like PR-proteins, virus resistance and R-gene proteins) leading to resistance response. The function of WRKY, NAC and MYB transcription factors in imparting the resistance against MYMIV could be established. The string analysis also revealed the role of proteins involved in kinase, viral movement and phytoene synthase activity in imparting YMD resistance. A set of novel stress-related EST-SSRs are also identified from the RNA-Seq data which may be used to find the linked genes/QTLs with the YMD resistance. Also, 11 defence-related transcripts could be validated through quantitative real-time PCR analysis. The identified gene networks have led to an insight about the defence mechanism operating against MYMIV infection in mungbean which will be of immense use to manage the YMD resistance in mungbean.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative RNA-Seq analysis unfolds a complex regulatory network imparting yellow mosaic disease resistance in mungbean [Vigna radiata (L.) R. Wilczek]

et al. 2021

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“…Globally, mungbean yellow mosaic virus (MYMV) remains a main constraint of mungbean production and management of this lethal disease is still the major challenge. Thus, finding ways to manage MYMV including identification and development of mungbean varieties possessing resistance against MYMV is a research priority for mungbean crop [2].…”

Section: Introductionmentioning

confidence: 99%

Screening of Mungbean (Vignaradiata L. Wilczek) Genotypes for Resistance to Mungbean Yellow Mosaic Virus under Field Condition

Gokidi

Singh

Singamsetti

et al. 2021

IJPSS

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Greengram [Vignaradiata(L.) Wilczek] is an economically important grain legume crop next to chickpea, pigeon pea and urad gram. Among the biotic factors, Mungbean Yellow Mosaic Virus (MYMV) is reported to be the most destructive viral diseases, which may reduce the seed quality as well as the yield losses up to 100 per cent. It is transmitted through whitefly (Bemisiatabaci) in a persistent manner throughout Asia, including India. A set of forty-two diverse mungbean genotypes were sown in two replications using a Randomized Block Desigh (RBD) during the summer season of 2015. The infector row method was adopted to evaluate a set of mungbean genotypes to know the difference in the level of resistance against MYMV infection under field condition during summer, 2015. Percent Disease Incidence (PDI) was calculated at 30 DAS and 50 DAS respectively. It varied from 2.18 to 64.77% and 5.38 to 76.87% at 30 DAS and 50 DAS respectively in summer, 2015. On the basis of disease severity recorded, the mungbean genotypes were classified in to five disease infection categories. Out of the forty-two mungbean genotypes, thirteen genotypes viz., Pusa 0672, IPM 205-7, HUM 8, KM 2245, IPM-2-03, ML 1464, KM 2241, PDM-139, TARM-1, HUM 26, Meha, HUM 16 and IPM 409-4 were found to be resistant and may provide the source of resistance against MYMV to develop mapping population for molecular breeding, development of molecular markers, QTL identification for MYMV resistance, as well as development of MYMV resistant varieties.

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“…The genome of begomovirus is bipartite and composed of circular single standard DNA-A and DNA-B of 2.7Kb each in size. The DNA-A encodes proteins required for replication, transcription and encapsidation, whereas DNA-B encodes nuclear shuttle protein and movement protein required for inter and intracellular movement of the virus and both DNA-A&B are required to cause yellow mosaic disease in blackgram and other pulses [3].…”

Section: Introductionmentioning

confidence: 99%

Distribution of Two Species of Begomoviruses Infecting Blackgram in Andhra Pradesh

Chaithanya¹,

Reddy

Prasanthi

et al. 2020

CJAST

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Background: Yellow mosaic diseases of blackgram is caused by two species of begomoviruses (MYMV, MYMIV). It is known fact that certain varities which are resistant to one species are susceptible to another species when screened by agroinoculations. Hence data on distribution of DNA-A &B components of MYMV / MYMIV under field conditions were not available in Andhra Pradesh. Methods: In the present study total eighty yellow mosaic infected blackgram samples were colleted from seven districts of Andhra Pradesh during rabi and kharif 2016-17. The total DNA was isolated and detected for presence DNA-A and B components of MYMV/ MYMIV by PCR with specific primers. Samples which showed negative in normal PCR are further detected by RCA-PCR. Results: PCR results showed that out of 80 YMD infected blackgram samples tested from seven districts, positve results were otained in 50% samples for MYMV-A, 98.75% for MYMIV-A,and 90% for MYMV-B and none for MYMIV-B. However RCA-PCR detected MYMIV-B presence in all 7 samples (8.25%) collected from Guntur district, but none from other six districts. These results clearly shows that both species of begoviruses are present in Andhra Pradesh and MYMIV-A is predominant species in Andhra Pradesh as compared to MYMV-A. Hence germplasm and breedig material should be screened for both the species of begomovirses either under field or through agroinoculations to develop YMV resistant blackgram.

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Yellow Mosaic Disease (YMD) of Mungbean (Vigna radiata (L.) Wilczek): Current Status and Management Opportunities

Cited by 46 publications

References 155 publications

Comparative RNA-Seq analysis unfolds a complex regulatory network imparting yellow mosaic disease resistance in mungbean [Vigna radiata (L.) R. Wilczek]

Comparative RNA-Seq analysis unfolds a complex regulatory network imparting yellow mosaic disease resistance in mungbean [Vigna radiata (L.) R. Wilczek]

Screening of Mungbean (Vignaradiata L. Wilczek) Genotypes for Resistance to Mungbean Yellow Mosaic Virus under Field Condition

Distribution of Two Species of Begomoviruses Infecting Blackgram in Andhra Pradesh

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