CO2-enriched atmosphere and supporting material impact the growth, morphophysiology and ultrastructure of in vitro Brazilian-ginseng [Pfaffia glomerata (Spreng.) Pedersen] plantlets

Saldanha, Cleber Witt; Otoni, Caio G.; Rocha, Diego Ismael; Cavatte, Paulo Cezar; Detmann, Kelly da Silva Coutinho; Tanaka, Francisco André Ossamu; Dias, Leonardo Lucas Carnevalli; DaMatta, Fábio M.; Otoni, Wagner Campos

doi:10.1007/s11240-014-0464-x

Cited by 38 publications

(18 citation statements)

References 62 publications

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“…Sucrose requirement by P. gardneriana plantlets in the culture medium was not suppressed by increased environmental photosynthetic photon flux densities Under light and gas exchange conditions sufficient for the plants to undergo photosynthesis in vitro, many species have the capacity to grow in the absence of sucrose in the culture medium, as observed in Pfaffia glomerata (Spreng) Pedersen (Iarema et al, 2012;Saldanha et al, 2014) and hybrids of orchid Doritaenopsis (Shin et al, 2013 ) decreases the requirement for sucrose in the culture medium and led to plantlets growth (Assis et al, 2016). However, in the present study, shoot formation of the P. gardneriana was dependent on sucrose addition in the culture medium.…”

Section: Discussionmentioning

confidence: 95%

“…Photoautotrophic techniques, including greater gas exchange between the environment and the inside of the culture flasks (Iarema et al, 2012;Saldanha et al, 2012), that enrich the atmosphere with CO 2 combined with or without more porous support materials (Saldanha et al, 2014), increase PPFD (Shin, Park, & Paek, 2013;Sáez, Bravo, Latsague, Sánchez, & Ríos, 2012) and decrease or eliminate sucrose from the culture medium (Xiao & Kozai, 2006) have been investigated. Studies that seek to improve the environmental conditions for the in vitro culture of native species help in understanding the optimal environmental factors to obtain plantlets with better performance during the acclimatization process.…”

Section: Morfoanatomia E Fisiologia De Plântulas De Pouteria Gardnerianamentioning

confidence: 99%

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<b>Morphoanatomy and physiology of Pouteria gardneriana Radlk plantlets grown in vitro at varied photosynthetic photon flux densities

et al. 2017

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ABSTRACT. Micropropagation is an important tool for the multiplication of native Cerrado species. However, understanding the responses of these species under in vitro culture conditions is still incomplete. Thus, the present study aimed to analyze the growth, anatomical behavior and physiology of Pouteria gardneriana cultivated in vitro under photoautotrophic conditions. Nodal segments were cultured at photosynthetic photon flux densities (PPFD) of 75, 100 and 150 μmol m de sacarose. Após 60 dias de cultivo in vitro observou-se regeneração de plântulas de P. gardneriana apenas quando a sacarose estava presente no meio de cultivo. Maior massa fresca e seca, maior espessura do parênquima paliçádico e diâmetro polar e equatorial foram observadas nas plântulas cultivadas no PPFD de 150 μmol m -2 s -1 . As diferentes PPFD utilizadas neste estudo foram suficientes para compreender o comportamento desta espécie in vitro.Palavras-chave: Cerrado, fotoautotrofismo, Sapotaceae.

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Section: Discussionmentioning

confidence: 95%

Section: Morfoanatomia E Fisiologia De Plântulas De Pouteria Gardnerianamentioning

confidence: 99%

<b>Morphoanatomy and physiology of Pouteria gardneriana Radlk plantlets grown in vitro at varied photosynthetic photon flux densities

et al. 2017

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“…Photoautotrophic propagation has recently been successfully used for some plant species (Cha-um et al 2011;Iarema et al 2012;Shin et al 2013;Saldanha et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Impacts of photoautotrophic and photomixotrophic conditions on in vitro propagated Billbergia zebrina (Bromeliaceae)

Martins

Verdoodt

Pasqual

et al. 2015

Plant Cell Tiss Organ Cult

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Micro-propagation techniques contribute to the multiplication of several bromeliad species. However, micropropagated plantlets often present low survival rate due to anatomical and physiological disorders induced by in vitro conditions. This study aimed to evaluate the sucrose and gas exchange impact on in vitro propagated Billbergia zebrina plants and to check if there is any residual effect of the in vitro conditions on micropropagated plants after acclimatization. Previously in vitro-established B. zebrina plants were transferred to culture media containing 0.0, 15.0, 30.0, 45.0 or 60.0 g L -1 sucrose. Two different culture container sealing systems were tested: lids with a filter (permitting an excellent gas exchange) and a filter covered with PVC (blocking fluent gas exchange). At 45 days in vitro growth, B. zebrina plantlets were transplanted onto plastic pots containing peat and cultivated for 80 days in greenhouse. At 45 days in vitro and 80 days of acclimatization in the greenhouse, the plants were evaluated. High sucrose levels in the in vitro media resulted in reduced growth. Plantlets exposed to aerated containers presented better rooting, being the sugar-free medium the best in vitro condition (photoautotrophic condition). Limited air exchange resulted in plantlets with anatomical and physiological disorders at the end of the in vitro period. The highest growth rate in the greenhouse was observed in plants previously propagated in unlimited gas exchange system and sugar-free medium. The use of photoautotrophic conditions induces B. zebrina plantlets without anatomical and physiological disorders and it interfere positively on ex vitro growth.

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“…MilliSeal ® membranes have been successfully used to improve gas exchange in the culture flask in the propagation of many species, including Pfaffia glomerata (IAREMA et al, 2012;SALDANHA et al, 2013SALDANHA et al, , 2014CORRÊA et al, 2015), Azadirachta indica (RODRIGUES et al, 2011), Alha gigraecorum (ZOBAYED et al, 2006), and Eucalyptus tereticornis (SHAVALLI KHAN et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Flask sealing on in vitro seed germination and morphogenesis of two types of ornamental pepper explants

et al. 2017

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CO2-enriched atmosphere and supporting material impact the growth, morphophysiology and ultrastructure of in vitro Brazilian-ginseng [Pfaffia glomerata (Spreng.) Pedersen] plantlets

Cited by 38 publications

References 62 publications

<b>Morphoanatomy and physiology of Pouteria gardneriana Radlk plantlets grown in vitro at varied photosynthetic photon flux densities

<b>Morphoanatomy and physiology of Pouteria gardneriana Radlk plantlets grown in vitro at varied photosynthetic photon flux densities

Impacts of photoautotrophic and photomixotrophic conditions on in vitro propagated Billbergia zebrina (Bromeliaceae)

Flask sealing on in vitro seed germination and morphogenesis of two types of ornamental pepper explants

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