2017
DOI: 10.4025/actasciagron.v39i2.32515
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<b>Morphoanatomy and physiology of Pouteria gardneriana Radlk plantlets grown in vitro at varied photosynthetic photon flux densities

Abstract: ABSTRACT. Micropropagation is an important tool for the multiplication of native Cerrado species. However, understanding the responses of these species under in vitro culture conditions is still incomplete. Thus, the present study aimed to analyze the growth, anatomical behavior and physiology of Pouteria gardneriana cultivated in vitro under photoautotrophic conditions. Nodal segments were cultured at photosynthetic photon flux densities (PPFD) of 75, 100 and 150 μmol m de sacarose. Após 60 dias de cultivo in… Show more

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Cited by 6 publications
(4 citation statements)
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References 33 publications
(35 reference statements)
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“…The mesophyll with dorsiventral disposition found in P. andarahiensis corroborates the description of Metcalfe & Chalk (1957) for the family and with the data found by Monteiro et al (2007), Almeida Jr. (2013; Leite et al (2017) and Lima et al (2019), for other species of Sapotaceae. It is possible to visualize sclerenchyma columns in the mesophyll, these columns together with the thick cuticle, may be related to long periods of drought, strong light and poor nutrient soil (Dickison 2000;Cutler et al 2011).…”
Section: Discussionsupporting
confidence: 91%
See 1 more Smart Citation
“…The mesophyll with dorsiventral disposition found in P. andarahiensis corroborates the description of Metcalfe & Chalk (1957) for the family and with the data found by Monteiro et al (2007), Almeida Jr. (2013; Leite et al (2017) and Lima et al (2019), for other species of Sapotaceae. It is possible to visualize sclerenchyma columns in the mesophyll, these columns together with the thick cuticle, may be related to long periods of drought, strong light and poor nutrient soil (Dickison 2000;Cutler et al 2011).…”
Section: Discussionsupporting
confidence: 91%
“…Regarding the diagnostic characters of the family, trichomes are numerous in young leaves of P. andarahiensis, and according to Press (1999) in hot and arid environments, the main role of trichomes is to decrease leaf temperature and water loss, decreasing the absorption of solar radiation, and hypoestomatic leaves with anisocytic stomata are in accordance with several works for the family and genus (Solereder 1908;Metcalfe & Chalk 1957;Pennington 2004;Monteiro et al 2007;Leite et al 2017), these stomata are numerous, which Wilkinson (1979) relates to xerophyte species.…”
Section: Discussionsupporting
confidence: 80%
“…En Capsicum chinense el empleo de alta intensidad de la luz 225 μmol/m -2 s -1 más 30 g/L de sacarosa en el medio de cultivo afectó los parámetros de crecimiento como longitud de brote, número de hojas, área foliar comparado con la intensidad de luz intensidad de la luz 28 μmol/m -2 s -1 (Barrales-López, 2015). La respuesta fotoautotrófica en las plántulas de pitahaya amarilla fue distinta a la reportada en Pouteria gardneriana que solo registraron regeneración de brotes cuando el medio de cultivo contenía como fuente de carbono la sacarosa, siendo indispensable la sacarosa en el medio de cultivo para el crecimiento y desarrollo in vitro de esta especie (Leite et al, 2017).…”
Section: Parámetrounclassified
“…En Hylocerus purpusii intensidad de la luz de 38 y 47,5 µmol m -2 s -1 y sacarosa 30 g/L (De Feria et al, 2012), Hylocereus monocanthus intensidad de la luz de 28 µmol m -2 s -1 y sacarosa 30 g/L (Montiel-Frausto et al, 2016), Selenicereus megalanthus intensidad de la luz de 37 µmol m -2 s -1 y sacarosa de 30 g/L (Suárez et al, 2014). La propagación in vitro bajo diferentes intensidades de la luz también ha sido evaluado en especies como Pouteria gardneriana registrando que la regeneración de plántulas es dependiente de la sacarosa en el medio de cultivo y que las intensidades de la luz mínimamente influenciaron en las características fenotípicas (Leite et al, 2017). Momordica grosvenori cultivada bajo condiciones fotoautotróficas registraron mejor desarrollo del sistema radicular, desarrollo de brotes alto contenido de clorofila, mayor capacidad fotosintética y alta supervivencia ex vitro (Zhang et al, 2009).…”
Section: Introductionunclassified