Co-occurrence of linear and cyclic pelgipeptins in broth cultures of Paenibacillus elgii AC13

Araújo, Thiago F.; Ortega, Daniel Barros; Costa, Rosiane Andrade da; Andrade, Isadora Emanoela Pereira Costa; Fulgêncio, Débora Luíza Albano; Mendonça, Marise Leite; Costa, Flávio Silva; Leite, Michel Lopes; Ramada, Marcelo Henrique Soller; Barreto, Cristine Chaves

doi:10.1007/s42770-021-00597-x

Cited by 3 publications

(2 citation statements)

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“…elgii. Interest in these molecules has increased over recent years due to their antimicrobial potential against human bacteria and fungi, − as well as against phytopathogenic microorganisms. , …”

Section: Discussionmentioning

confidence: 99%

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Pelgipeptins, a Nonribosomal Lipopeptide Family, Show Larvicidal Activity against Vectors Transmitting Viruses

Costa

Dutra

Andrade

et al. 2023

J. Agric. Food Chem.

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Aedes aegypti and Culex quinquefasciatus are vectors of numerous diseases of worldwide public importance, such as arboviruses and filariasis. The main strategy for controlling these vectors is the use of chemicals, which can induce the appearance of resistant insects. The use of Bacillus thuringiensis (Bt) and Lysinibacillus sphaericus (Ls) with larvicidal activity against arboviraltransmitting insects has been successful in many studies. In contrast, the use and knowledge of peptides with insecticidal activity are so far scarce. In this work, 25 peptides and 5 strains of each bacterial species were prospected individually or together regarding their insecticidal activity. Initially, in vitro assays of cellular cytotoxicity of the peptides against SF21 cells of Spodoptera frugiperda were performed. The peptides Polybia-MPII and pelgipeptin caused 69 and 60% of cell mortality, respectively, at the concentration of 10 μM. Thus, they were evaluated in vivo against second-stage larvae of the two Culicidae. However, in the in vivo bioassays, only pelgipeptin showed larvicidal mortality against both larvae (LC 50 6.40 μM against A. aegypti, and LC 50 1.22 μM against C. quinquefasciatus). The toxin-producing bacterial strain that showed the lowest LC 50 against A. aegypti was Bt S8 (LC 50 = 0.71 ng/ mL) and against C. quinquefasciatus, it was Ls S260 (LC 50 = 2.32 ng/mL). So, the synergistic activity between the association of the bacterial toxins and pelgipeptin was evaluated. A synergic effect of pelgipeptin was observed with Ls strain S260 against C. quinquefasciatus. Our results demonstrate the possibility of synergistic or individual use of both biologically active larvicides against C. quinquefasciatus and A. aegypti.

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Section: Discussionmentioning

confidence: 99%

“…quinquefasciatus (Table ). The Paenibacillus elgii AC13 strain was isolated from Cerrado soil and is known for producing lipopeptides belonging to the pelgipeptin family, which presents antimicrobial activity. − …”

Section: Methodsmentioning

confidence: 99%