Aedes aegypti and Culex quinquefasciatus are vectors of numerous diseases of worldwide public importance, such as arboviruses and filariasis. The main strategy for controlling these vectors is the use of chemicals, which can induce the appearance of resistant insects. The use of Bacillus thuringiensis (Bt) and Lysinibacillus sphaericus (Ls) with larvicidal activity against arboviraltransmitting insects has been successful in many studies. In contrast, the use and knowledge of peptides with insecticidal activity are so far scarce. In this work, 25 peptides and 5 strains of each bacterial species were prospected individually or together regarding their insecticidal activity. Initially, in vitro assays of cellular cytotoxicity of the peptides against SF21 cells of Spodoptera frugiperda were performed. The peptides Polybia-MPII and pelgipeptin caused 69 and 60% of cell mortality, respectively, at the concentration of 10 μM. Thus, they were evaluated in vivo against second-stage larvae of the two Culicidae. However, in the in vivo bioassays, only pelgipeptin showed larvicidal mortality against both larvae (LC 50 6.40 μM against A. aegypti, and LC 50 1.22 μM against C. quinquefasciatus). The toxin-producing bacterial strain that showed the lowest LC 50 against A. aegypti was Bt S8 (LC 50 = 0.71 ng/ mL) and against C. quinquefasciatus, it was Ls S260 (LC 50 = 2.32 ng/mL). So, the synergistic activity between the association of the bacterial toxins and pelgipeptin was evaluated. A synergic effect of pelgipeptin was observed with Ls strain S260 against C. quinquefasciatus. Our results demonstrate the possibility of synergistic or individual use of both biologically active larvicides against C. quinquefasciatus and A. aegypti.
O bioma Cerrado ocupa cerca de 23% da área brasileira e é considerado o segundo maior bioma do Brasil. Possui cerca de 4.800 espécies endêmicas, incluindo a espécie frutífera Eugenia dysenterica DC. (cagaita). Rica em nutrientes, esta espécie possui potencial econômico no Brasil por possuir ações diretamente relacionadas à saúde humana. O presente estudo realizou o monitoramento tecnológico dos conhecimentos e das tecnologias geradas, utilizando a espécie E. dysenterica, no período de 2009-2019. Os dados demostraram que durante esse período foram encontrados para esta espécie 77 dissertações, 21 teses, 97 artigos científicos e duas patentes depositadas. Uma análise mais detalhada demonstrou que parte do conhecimento e das tecnologias geradas está concentrada na área de biotecnologia ambiental (35,9%), seguida pela biotecnologia industrial (28,3%), biotecnologia da saúde (22,8%) e biotecnologia vegetal (13%). Assim, o artigo analisa e discute o potencial a ser explorado em relação à E. dysenterica para o desenvolvimento de produtos biotecnológicos de interesse comercial.
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