Co-located hAT transposable element and 5S rDNA in an interstitial telomeric sequence suggest the formation of Robertsonian fusion in armored catfish

Glugoski, Larissa; Giuliano-Caetano, Lúcia; Moreira‐Filho, Orlando; Vicari, Marcelo Ricardo; Nogaroto, Viviane

doi:10.1016/j.gene.2018.01.099

Cited by 45 publications

(51 citation statements)

References 53 publications

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“…These nonspecific data can be explained by the abundance of repetitive elements in the O. octoguttata genome. Gene conversion, unequal crossover, and the nonallelic homologous recombination mechanisms mixed different TE sequences into the O. octoguttata genome, as also observed in fishes [Schemberger et al, 2014;Glugoski et al, 2018]. In addition to the accumulation of TEs in each genome, the comparative mapping of TEs among O. octoguttata , O. sexnotata , and O. personata demonstrated differential chromosomal accumulation sites, especially when the TE distributions in the X and Y sex chromosomes were compared.…”

Section: Discussionmentioning

confidence: 61%

“…They are involved in the accumulation of paralogous sequences and expansion of genome sizes. Sometimes, they act as hotspots for DNA double-strand breaks, which promote chromosomal rearrangements [Kidwell andLisch, 2000, 2001;Kidwell, 2002;Barros et al, 2017;Glugoski et al, 2018]. The TEs hobo , BEL, element P , roo , HeT-A , TART, Mariner, and Tango of Drosophila were involved in several chromosomal rearrangements and karyotypic diversification [Lim and Simmons, 1994;Gray, 2000;Lönnig and Saedler, 2002].…”

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confidence: 99%

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Accumulation of Transposable Elements in Autosomes and Giant Sex Chromosomes of Omophoita (Chrysomelidae: Alticinae)

Rosolen

Vicari

Almeida

2018

Cytogenet Genome Res

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Coleoptera is the most diverse order among insects, and comparative molecular cytogenetic studies in this group are lacking. The species of Omophoita (Oedionychina) possess a karyotype of 2n = 22 = 10II+X+Y. They are interesting models for evolutionary cytogenetic studies due to giant sex chromosomes which are asynaptic during meiosis. Transposable elements (TEs) confer plasticity and mobility to genomes and are considered hotspots for DNA double-strand breaks and chromosomal rearrangements. The objective of the present study was to verify the role of TEs in the karyotype and in the size expansion of the giant sex chromosomes in Omophoita. Thus, different TEs were characterized in the Omophoita genome and localized in the chromosomes by fluorescence in situ hybridization (FISH). The DNA sequencing data revealed identity with TE families Tc1/Mariner and RTE/L1-56_XT. FISH showed signals of all TEs in the karyotypes and a high accumulation in the sex chromosomes of the 3 Omophoita species analyzed. These data suggest that the genome size expansion and the origin of the giant sex chromosomes of Omophoita are due to an intensive genomic invasion of TEs, as those characterized here as Tc1/Mariner-Ooc and RTE-Ooc. Differences in the chromosomal location of the TEs among the 3 species indicate that they have participated in the karyotype differentiation in Omophoita.

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Section: Discussionmentioning

confidence: 61%

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confidence: 99%

Accumulation of Transposable Elements in Autosomes and Giant Sex Chromosomes of Omophoita (Chrysomelidae: Alticinae)

Rosolen

Vicari

Almeida

2018

Cytogenet Genome Res

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“…Moreover, a Tc1 retrotransposon located within NTS of the 5S rDNA has been proposed as the agent that causes the large number of 5S rDNA loci observed in Gymnotus mamiraua . Similarly, a Rex retroelement and a hAT transposon have been suggested as the causative for the 5S rDNA dispersion in Ancistrus and Rinelocaria species, respectively (Favarato et al, 2016;Glugoski et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…According to Drouin (2000), the presence of TEs can be responsible for rRNA gene movements, and this process can be displayed by the cytogenetic analysis performed here and in previous studies. Moreover, it has been stated that a double strand break within the 5S array, followed by a non-homologous recombination repair mechanism during prophase, are events that lead to share 5S sequences among chromosomes (Barros et al, 2017;Glugoski et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Evolutionary Dynamics of Multigene Families in Triportheus (Characiformes, Triportheidae): A Transposon Mediated Mechanism?

et al. 2020

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Triportheus (Characiformes, Triportheidae) is a freshwater fish genus with 18 valid species. These fishes are widely distributed in the major river drainages of South America, having commercial importance in the fishing market, mainly in the Amazon basin. This genus has diverged recently in a complex process of speciation carried out in different river basins. The use of repetitive sequences is suitable to trace the genomic reorganizations occured along the speciation process. In this work, the 5S rDNA multigene family has been characterized at molecular and phylogenetic level. The results showed that other multigene family has been found within the non-transcribed spacer (NTS): the U1 snRNA gene. Double-FISH with 5S and U1 probes were also performed, confirming the close linkage between these two multigene families. Moreover, evidences of different transposable elements (TE) were detected within the spacer, thus suggesting a transposon-mediated mechanism of 5S-U1 evolutionary pathway in this genus. Phylogenetic analysis demonstrated a species-specific grouping, except for Triportheus pantanensis, Triportheus aff. rotundatus and Triportheus trifurcatus. The evolutionary model of the 5S rDNA in Triportheus species has been discussed. In addition, the results suggest new clues for the speciation and evolutionary trend in these species, which could be suitable to use in other Characiformes species.

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“…From the cytogenetic standpoint only, certain repetitive DNA markers, including 5S and 18S rDNA, have been formerly found to be involved in the formation of centric fusions (e.g., [39][40][41]). In the case of rDNAs, this may be possibly linked with the susceptibility of these tandemly repeated clusters to double-stranded DNA breaks, perhaps resulting from (1) a frequent rRNA transcription and thus break-prone R-loop emergence, (2) intermingling of NOR (Nuclear Organizer Region)-bearing chromosomes in the interphase nucleus, or (3) possible association of rDNA-bearing sites during the meiotic prophase I [42][43][44][45][46][47][48].…”

Section: Discussionmentioning

confidence: 99%

Centric Fusions behind the Karyotype Evolution of Neotropical Nannostomus Pencilfishes (Characiforme, Lebiasinidae): First Insights from a Molecular Cytogenetic Perspective

Sember

Oliveira

Ráb

et al. 2020

Genes

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Lebiasinidae is a Neotropical freshwater family widely distributed throughout South and Central America. Due to their often very small body size, Lebiasinidae species are cytogenetically challenging and hence largely underexplored. However, the available but limited karyotype data already suggested a high interspecific variability in the diploid chromosome number (2n), which is pronounced in the speciose genus Nannostomus, a popular taxon in ornamental fish trade due to its remarkable body coloration. Aiming to more deeply examine the karyotype diversification in Nannostomus, we combined conventional cytogenetics (Giemsa-staining and C-banding) with the chromosomal mapping of tandemly repeated 5S and 18S rDNA clusters and with interspecific comparative genomic hybridization (CGH) to investigate genomes of four representative Nannostomus species: N. beckfordi, N. eques, N. marginatus, and N. unifasciatus. Our data showed a remarkable variability in 2n, ranging from 2n = 22 in N. unifasciatus (karyotype composed exclusively of metacentrics/submetacentrics) to 2n = 44 in N. beckfordi (karyotype composed entirely of acrocentrics). On the other hand, patterns of 18S and 5S rDNA distribution in the analyzed karyotypes remained rather conservative, with only two 18S and two to four 5S rDNA sites. In view of the mostly unchanged number of chromosome arms (FN = 44) in all but one species (N. eques; FN = 36), and with respect to the current phylogenetic hypothesis, we propose Robertsonian translocations to be a significant contributor to the karyotype differentiation in (at least herein studied) Nannostomus species. Interspecific comparative genome hybridization (CGH) using whole genomic DNAs mapped against the chromosome background of N. beckfordi found a moderate divergence in the repetitive DNA content among the species’ genomes. Collectively, our data suggest that the karyotype differentiation in Nannostomus has been largely driven by major structural rearrangements, accompanied by only low to moderate dynamics of repetitive DNA at the sub-chromosomal level. Possible mechanisms and factors behind the elevated tolerance to such a rate of karyotype change in Nannostomus are discussed.

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Co-located hAT transposable element and 5S rDNA in an interstitial telomeric sequence suggest the formation of Robertsonian fusion in armored catfish

Cited by 45 publications

References 53 publications

Accumulation of Transposable Elements in Autosomes and Giant Sex Chromosomes of <b><i>Omophoita</i></b> (Chrysomelidae: Alticinae)

Accumulation of Transposable Elements in Autosomes and Giant Sex Chromosomes of <b><i>Omophoita</i></b> (Chrysomelidae: Alticinae)

Evolutionary Dynamics of Multigene Families in Triportheus (Characiformes, Triportheidae): A Transposon Mediated Mechanism?

Centric Fusions behind the Karyotype Evolution of Neotropical Nannostomus Pencilfishes (Characiforme, Lebiasinidae): First Insights from a Molecular Cytogenetic Perspective

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