Co-infection of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) caused high mortality in farmed rainbow trout (Oncorhynchus mykiss) in China

Xu, Liming; Zhao, Juanjuan; Ren, Guoqing; Dong, Ying; Lin, Jingnan; Cao, Yanghui; Yin, Jing; Liu, Hongbai; Lü, Tao; Zhang, Qi-Ya

doi:10.1016/j.aquaculture.2019.734286

Cited by 17 publications

(4 citation statements)

References 28 publications

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“…Same as in our previous study [27], virus RNA was extracted by using Trizol reagent (Invitrogen, Shanghai, China), which was obtained by the CHSE-214 cell culture. Briefly add 1 mL of Trizol reagent per suspension and incubate for 5 min.…”

Section: Extraction Of Viral Genome Rnamentioning

confidence: 99%

“…The infection titers of tissues and cell culture suspensions to be tested were determined by routine procedures on CHSE-214 monolayers [27]. The TCID 50 of the infection samples was calculated according to the Reed and Muench method [28], the TCID 50 of cell culture suspensions was expressed as TCID 50 /mL, and the TCID 50 of tissue samples was expressed as TCID 50 /g.…”

Section: Titrationmentioning

confidence: 99%

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Dynamic Distribution of Infectious Pancreatic Necrosis Virus (IPNV) Strains of Genogroups 1, 5, and 7 after Intraperitoneal Administration in Rainbow Trout (Oncorhynchus mykiss)

Shao

Ren

Zhao

et al. 2022

Viruses

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Infectious pancreatic necrosis virus (IPNV) is the causative agent of rainbow trout (Oncorhynchus mykiss) IPN and causes significant loss of fingerlings. The currently prevalent IPNV genogroups in China are genogroups 1 and 5. However, in this study, we isolated and identified a novel IPNV, IPNV-P202019, which belonged to genogroup 7. Here, a total of 200 specific-pathogen-free rainbow trout (10 g average weight) were divided randomly into four groups to investigate the distribution of different IPNV strains (genogroups 1, 5, and 7) in 9 tissues of rainbow trout by means of intraperitoneal (ip) injection. Fish in each group were monitored after 3-, 7-, 14-, 21- and 28- days post-infection (dpi). The study showed no mortality in all groups. The distribution of IPNV genogroups 1 and 5 was similar in different tissues and had a higher number of viral loads after 3, 7, or 14 dpi. However, the distribution of IPNV genogroup 7 was detected particularly in the spleen, head kidney, and feces and had a lower number of viral loads. The results of this study provide valid data for the distribution of IPNV in rainbow trout tissues and showed that IPNV genogroups 1 and 5 were still the prevalent genogroups of IPNV in China. Although rainbow trout carried IPNV genogroup 7, the viral load was too low to be pathogenic.

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Section: Extraction Of Viral Genome Rnamentioning

confidence: 99%

Section: Titrationmentioning

confidence: 99%

Dynamic Distribution of Infectious Pancreatic Necrosis Virus (IPNV) Strains of Genogroups 1, 5, and 7 after Intraperitoneal Administration in Rainbow Trout (Oncorhynchus mykiss)

Shao

Ren

Zhao

et al. 2022

Viruses

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“…Genogroup 1 isolates have also been found in farmed salmonids affected by IPN in China [97][98][99] and Japan. 29 The Japanese and Chinese isolates from genogroup 1 showed great similarity and were clustered together with viruses from the USA and Mexico.…”

Section: F I G U R Ementioning

confidence: 99%

Infectious pancreatic necrosis virus in salmonids: Molecular epidemiology and host response to infection

Tapia

Eissler

Reyes-López

et al. 2021

Reviews in Aquaculture

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One of most important viral diseases that affect salmonid aquaculture worldwide is Infectious Pancreatic Necrosis (IPN), a contagious disease that can cause high mortality rates in first-feeding fry, and in post-smolts shortly after transfer to seawater. 1,2 Its aetiological agent, IPN virus (IPNV), is an Aquabirnavirus (within the family Birnaviridae), and is considered the most pervasive pathogens of aquatic animals, with worldwide distribution and a wide range of hosts. 3,4 The term "IPN virus," however, has been used (and will be used in this review) to denote only aquabirnaviruses infecting or causing disease in salmonids. 4 Aquatic birnaviruses isolated from non-salmonid species will be referred to as marine aquabirnaviruses (MABV) or yellowtail ascites virus (YTAV) if they were isolated or caused disease in yellowtail fish (Seriola quinqueradiata). 5 The salmonid species typically affected by IPN are brook trout (Salvelinus

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“…Moreover, fresh S. salar with smooth taste and bright color is often served in raw by the means of sashimi . However, O. mykiss is generally not suitable for raw eating due to the health concerns by parasite infections and excessive accumulation of heavy metals ( Barrientos et al, 2019 , Xu et al, 2019 ). In addition, the freshwater-cultured O. mykiss was often reported to have earthy and musty off-flavors arising from the geosmin and 2-methylisoborneol ( Lindholm-Lehto et al, 2019 ), and its price (about 20 RMB/kg, or 3 USD/kg) is generally much lower than S. salar (about 110 RMB/kg or 16 USD/kg) in China (author’s note).…”

Section: Introductionmentioning

confidence: 99%

Visual detection of rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar) simultaneously by duplex loop-mediated isothermal amplification

Xue

Fei

et al. 2022

Food Chemistry: Molecular Sciences

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Co-infection of infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) caused high mortality in farmed rainbow trout (Oncorhynchus mykiss) in China

Cited by 17 publications

References 28 publications

Dynamic Distribution of Infectious Pancreatic Necrosis Virus (IPNV) Strains of Genogroups 1, 5, and 7 after Intraperitoneal Administration in Rainbow Trout (Oncorhynchus mykiss)

Dynamic Distribution of Infectious Pancreatic Necrosis Virus (IPNV) Strains of Genogroups 1, 5, and 7 after Intraperitoneal Administration in Rainbow Trout (Oncorhynchus mykiss)

Infectious pancreatic necrosis virus in salmonids: Molecular epidemiology and host response to infection

Visual detection of rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar) simultaneously by duplex loop-mediated isothermal amplification

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