Co-fermentation of a mixture of glucose and xylose to fumaric acid by Rhizopus arrhizus RH 7 - 13-9#

Liu, Huan; Hu, Huirong; Jin, Yuhan; Yue, Xuemin; Deng, Li; Wang, Fang; Tan, Tianwei

doi:10.1016/j.biortech.2017.02.035

Cited by 34 publications

(11 citation statements)

References 17 publications

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“…The acceleration of monosaccharide consumption, even without the utilization of glycerol from the co-fermentation media, seems to be a very positive aspect of the strategy suggested in this paper. Although the transportation mechanism of glycerol molecules into fungal cells had already been described (Fakas et al 2009 ; Klein et al 2017 ), as well as glucose-xylose co-utilization by Rhizopus (Liu et al 2017 ), an in-depth analysis of the processes in the presence of monosaccharides-glycerol mixtures should be made for a better understanding of the entire mechanism. This work may be a good start to advanced research on Rhizopus species metabolism under specific co-utilization conditions.…”

Section: Discussionmentioning

confidence: 99%

A co-utilization strategy to consume glycerol and monosaccharides by Rhizopus strains for fumaric acid production

et al. 2018

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The ability of Rhizopus oryzae to produce fumaric acid in the presence of glycerol and/or various monosaccharides as carbon sources was examined for seventeen different strains of this fungi. These strains were tested in shake-flask cultures on media containing glycerol and seven different carbohydrates, including glucose, fructose, galactose, mannose, xylose, arabinose, and rhamnose. An interesting and applicationally useful phenomenon was observed. This work presents a new approach to the conventional microbiological method of producing fumaric acid. In the presence of 40 g/l glycerol as the sole carbon source, fumaric acid production reached 0.16–6.1 g/l after 192 h. When monosaccharides were used as a single carbon source, the maximum fumaric acid concentration was much higher; for example, 19.8 g/l was achieved when 40 g/l xylose was used. In the co-fermentation of xylose (40 g/l) and glycerol (20 g/l), post-culture broth contained approx. 28.0 g/l of fumaric acid with a process yield of 0.90 g/g after 168 h. The production of fumaric acid by Rhizopus oryzae was also increased in the dual presence of glycerol and monosaccharides like fructose, galactose, and mannose. However, results obtained on glucose-glycerol-based medium did not follow this trend, showing instead complete utilization of glucose with significant glycerol consumption, but unexpectedly low final amounts of fumaric acid and process yields. Understanding how Rhizopus oryzae utilize various carbon sources may provide alternative avenues of fumaric acid fermentation.

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Section: Discussionmentioning

confidence: 99%

A co-utilization strategy to consume glycerol and monosaccharides by Rhizopus strains for fumaric acid production

et al. 2018

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“…The column was eluted with 0.005 M H2SO4 at a column temperature of 50 °C and a flow rate of 0.6 mL/min (Liu et al 2017a). The p-values of the data shown in this work were p< 0.05 after significance testing.…”

Section: Analysis Methodsmentioning

confidence: 74%

Production of cellulase by Microbulbifer hydrolyticus through co-fermentation of glucose and xylose from lignocellulose

Liu

Xiao

et al. 2020

BioRes

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Cellulase is a compound enzyme that catalyzes cellulose into monosaccharides or oligosaccharides. Large amounts of cellulase are needed with the development of the lignocellulose processing industry, which necessitates faster methods to produce cellulase. In this work, the marine bacterium Microbulbifer hydrolyticus IRE-31-192 was selected to produce cellulase, due to its fast growth rate and short high space-time yield. Co-fermentation of glucose and xylose to produce cellulase was investigated on the basis of previous work. When the ratio of glucose/xylose was 2:1 (w/w), 294 U/L cellulase activity with highest space-time yield of 12.2 U/L h was obtained. The hydrolytic liquid of lignocellulose prepared from dried distiller’s grains with solubles (DDGS) with the similar ratio of glucose/xylose was used as medium to produce cellulase. The efficiency of cellulase production from processed and unprocessed hydrolysates of DDGS was compared. Unprocessed hydrolysates were more beneficial for the production of cellulase, such that its activity was 261 U/L with a space-time yield of 14.5 U/L h. Thus, commonly used pure glucose and xylose could be replaced by hydrolysates of DDGS, and marine bacteria has potential application for cellulase production.

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“…As the second abundant sugar in lignocellulose hydrolysate after glucose, xylose makes up 30–40% of lignocellulosic biomass. , Although many microorganisms can use both sugars, glucose is generally preferred to be utilized due to GCR . To improve the utilization of lignocellulosic hydrolysates, studies have achieved the co-utilization of glucose and xylose by removing GCR through genetic engineering. , However, the existence of GCR endows the host strains unique advantages. Especially, the preference to the utilization of glucose greatly facilitates the cell growth of host strains, which is also true for the thermophile G.…”

Section: Discussionmentioning

confidence: 99%

Dynamic Control Strategy to Produce Riboflavin with Lignocellulose Hydrolysate in the Thermophile Geobacillus thermoglucosidasius

Wang

et al. 2022

ACS Synth. Biol.

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The e cient utilization of both glucose and xylose, the two most abundant sugars in biomass hydrolysates, is one of the main objectives of biofermentation with lignocellulosic materials. The utilization of xylose is commonly inhibited by glucose, which is known as glucose catabolite repression (GCR). Here we report a GCR-based dynamic control (GCR-DC) strategy aiming at a better co-utilization of glucose and xylose, by decoupling the cell growth and biosynthesis of ribo avin as a product. Using the thermophilic strain Geobacillus thermoglucosidasius DSM2542 as a host, we constructed extra ribo avin biosynthetic pathways that were activated by xylose but not glucose. The engineered strains showed a two-stage fermentation process. In the rst stage, glucose was preferentially used for cell growth and no production of ribo avin was observed, while in the second stage where glucose was nearly depleted, xylose was effectively utilized for ribo avin biosynthesis. Using the corn cob hydrolysate as a carbon source, the optimized ribo avin yields of strains DSM2542-DCall-MSS (full pathway dynamic control strategy) and DSM2542-DCrib (single module dynamic control strategy) were 5.26 and 2.26 folds higher than that of the control strain DSM2542 Rib-Gtg constitutively producing ribo avin, respectively. This GCR-DC strategy should also be applicable to the construction of cell factories that can e ciently use natural carbon sources with multiple sugar components for the production of high-value chemicals in future.

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Co-fermentation of a mixture of glucose and xylose to fumaric acid by Rhizopus arrhizus RH 7 - 13-9#

Cited by 34 publications

References 17 publications

A co-utilization strategy to consume glycerol and monosaccharides by Rhizopus strains for fumaric acid production

A co-utilization strategy to consume glycerol and monosaccharides by Rhizopus strains for fumaric acid production

Production of cellulase by Microbulbifer hydrolyticus through co-fermentation of glucose and xylose from lignocellulose

Dynamic Control Strategy to Produce Riboflavin with Lignocellulose Hydrolysate in the Thermophile Geobacillus thermoglucosidasius

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