Co-enzyme Q10 and acetyl salicylic acid enhance Hsp70 expression in primary chicken myocardial cells to protect the cells during heat stress

Xu, Jiao; Tang, Shu; Yin, Bin; Sun, Jiarui; Song, Erwei; Bao, Endong

doi:10.1007/s11010-017-3058-1

Cited by 23 publications

(19 citation statements)

References 48 publications

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“…To this extent, some studies have reported that, in responses to oxidative stress, inducing HSPs with physiological stimulus such as exercise can improve tissue protection [40][41][42]. In this study, aspirin, a well-known anti-fever drug, was proved to induce successfully increased Hsp90 and Hsp70 expression in kidneys with or without heat stress exposure, similar to our studies in myocardial cells [43,44]. The induction of Hsp90 and Hsp70 by aspirin might occur through regulating the expression and nuclear translocation of HSF-1 and HSF-3 [30,43,44].…”

Section: Discussionsupporting

confidence: 86%

“…In this study, aspirin, a well-known anti-fever drug, was proved to induce successfully increased Hsp90 and Hsp70 expression in kidneys with or without heat stress exposure, similar to our studies in myocardial cells [43,44]. The induction of Hsp90 and Hsp70 by aspirin might occur through regulating the expression and nuclear translocation of HSF-1 and HSF-3 [30,43,44]. Under this condition, the inhibition of cellular apoptosis and enhancement of autophagy were found, including milder tissue injury.…”

Section: Discussionsupporting

confidence: 84%

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Hsp90 Relieves Heat Stress-Induced Damage in Mouse Kidneys: Involvement of Antiapoptotic PKM2-AKT and Autophagic HIF-1α Signaling

Chen

Yang

Zhu

et al. 2020

IJMS

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Heat stress can particularly affect the kidney because of its high rate of adenosine triphosphate consumption. Competition between apoptosis and autophagy-mediated survival always exists in damaged tissue. And Hsp90 can enhance cellular protection to resist heat stress. However, the relationship between Hsp90 and the above competition and its underlying mechanism in the kidney are unclear. The present study found that heat stress induced obvious histopathological and oxidative injury, which was connected with cellular apoptosis and autophagy in the kidney and was associated with the levels of Hsp90 expression or function. The data showed that during heat stress, Hsp90 activated the PKM2-Akt signaling pathway to exert antiapoptotic effects and induce Hsp70 expression regulated by HSF-1, stimulated autophagy-mediated survival through the HIF-1α-BNIP3/BNIP3L pathway, and finally protected the kidney from heat-stress injury. Moreover, the nuclear translocation of PKM2, (p-) Akt, HSF-1, and HIF-1α was enhanced by heat stress, but only intranuclear p-Akt and HSF-1 were specifically influenced by Hsp90, contributing to regulate the cellular ability of resisting heat-stress damage. Our study provided new insights regarding the molecular mechanism of Hsp90 in the kidney in response to heat-stress injury, possibly contributing to finding new targets for the pharmacological regulation of human or animal acute kidney injury from heat stress in future research.Autophagy, a lysosomal degradation pathway, is an essential cellular adaptation mechanism for avoiding oxidative damage. Studies of the role of autophagy in AKI have reported both beneficial and detrimental effects [12][13][14]. Light chain 3 (LC3), is essential for autophagy and serves as a vital component in monitoring autophagy and visualizing autophagosomes in vivo [15]. Pyruvate kinase M2 isoform (PKM2), a rate-limiting terminal glycolytic enzyme, catalyzes the last step of glycolysis, and recent studies have reported that PKM2 is associated with the Akt signal pathway, which regulates cell survival and apoptosis [16,17]. Meanwhile, many cellular signals have been reported to be involved in the regulation of autophagy, including the Akt (protein kinase B)-mTOR (mammalian target of rapamycin) pathway [18,19]. Furthermore, Akt also plays a vital role in resisting heat stress-induced apoptosis [20]. Hypoxia-inducible factor (HIF), especially HIF-1α, is also a major actor in the cell survival autophagy in response to hypoxia stress via BNIP3 (Bcl-2/adenovirus E1B 19-kDa interacting protein 3) and BNIP3L (Bcl-2/adenovirus E1B 19-kDa interacting protein 3 like) [21]. However, understanding the roles and their interplay of autophagy and apoptosis, and identification of the closely related signaling pathways in the kidney, is still very deficient but important for controlling tissue damage during heat stress.At the cellular level, tolerance to heat stress is mainly regulated by heat shock proteins (HSPs), which are also synthesized under various environmental and oxidative...

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 84%

Hsp90 Relieves Heat Stress-Induced Damage in Mouse Kidneys: Involvement of Antiapoptotic PKM2-AKT and Autophagic HIF-1α Signaling

Chen

Yang

Zhu

et al. 2020

IJMS

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“…Many radicals and metabolic substances are potentially toxic and are defined as “reactive oxygen/nitrogen/chlorine species”[ 4 , 9 ]. These substances are highly reactive and can modify several biologically cellular macromolecules, such as proteins, lipids, and nucleic acids (DNA and RNA) [ 10 , 11 ]. Heat stress is one of the most challenging environmental conditions that affect commercial poultry.…”

Section: Introductionmentioning

confidence: 99%

“…The chickens showed a reduced heat shock phenomenon in vivo, and their primary myocardial cells showed less damage compared with those of the HS-only group. In addition, indicators of oxidative stress, such as malondialdehyde (MDA), superoxide dismutase (SOD), and lactate dehydrogenase (LDH), were decreased after Q10 and vitamin C treatment [ 10 , 14 ]. Therefore, we focused on identifying further natural substances that can ease HS of broilers in vivo.…”

Section: Introductionmentioning

confidence: 99%

Rosemary Reduces Heat Stress by Inducing CRYAB and HSP70 Expression in Broiler Chickens

Tang

Yin

et al. 2018

Oxidative Medicine and Cellular Longevity

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Heat stress negatively affects poultry production and animal health. In response, animals invoke a heat stress response by inducing heat shock proteins (HSPs). Scientists are actively seeking natural products that can enhance the heat shock response. The present study aimed at assessing the effects of a purified rosemary extract comprising antioxidant compounds on the heat shock response and HSP expression profile in broiler chickens. The response of broilers to HS in the presence of purified rosemary extract was assessed using an in vivo myocardial cell model. Pathological lesions of heart tissue were examined microscopically. The levels and activities of enzymes associated with heart damage and oxidative damage were detected. Immunohistochemical staining was performed for HSPs in myocardial cells. The results showed that lactate dehydrogenase (LDH), creatine kinase (CK), and myocardial CK (CKMB) levels were reduced by the purified rosemary extract before and during heat stress. Heat stress alone increased CK and CKMB levels. The levels of oxidative damage-associated enzymes were compared between the rosemary + heat stress and heat stress-alone groups. The results indicated that in terms of these enzymes, the purified rosemary extract induced a more antioxidative state. Pathological examinations showed that heat stress caused myocardial fiber fracture, karyopyknosis, and degeneration. The addition of purified rosemary extract ameliorated these lesions to some degree, preserving more of the basic structure. Heat stress decreased the cellular levels of crystallin alpha B (CRYAB) and HSP70. The addition of the purified rosemary extract significantly increased the levels of CRYAB and HSP70 during heat stress (p < 0.0001). Immunohistochemistry showed that after rosemary treatment, CRYAB and HSP70 showed more intense staining compared with the no heat stress control group. In the rosemary + heat group, after 10 hours of heat stress, the staining intensity of these two proteins remained higher than in the heat stress group. Thus, purified rosemary extract could induce high levels of HSP70 and CRYAB in chicken hearts before and during heat stress. Purified rosemary extract could be used to alleviate heat stress in broiler chickens.

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“…Виходячи з цих даних, вікасол може викликати в міокарді зміни жирнокислотного складу тканин, а правильно підібрана доза препарату забезпечить підвищення опірності організму до негативних чинників, що може бути використано при виборі технології годування та лікування сільськогосподарської птиці. У той же час позитивний ефект на функціональний стан міокарду підтверджується широким застосуванням хінонів як кардіопротекторних препаратів [9,15,21,34].…”

Section: біохіміяunclassified

Жирнокислотний Склад Міокарду Гусей За Дії Вікасолу

Яковійчук¹,

Данченко²,

Danchenko³

et al. 2019

TernopilBioSciIssues

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In science vicasol is known to be relative to the energy and antioxidant systems of tissues closely related to the biosynthesis and oxidation of fatty acids. This effect may cause changes in the fatty acid composition of tissues, and numerous works on the positive effect of quinones and their derivatives on the myocardial function, suggest that the proper dose and feeding can increase the stability and productivity of poultry. The given was aims to study the effect of vicasol on the fatty acid composition of goose myocardium. Myocardium was chosen as a biological object. Biological material was collected every 7 days throughout the period from the 21st to the 35th day of ontogeny, characterized by the state of physiological tension of geese. Feeding of geese with vicasol at a dose of 0.7 mg / kg body weight began with the 3rd day of ontogeny. Fatty acid analysis in myocardial tissues was performed by gas-liquid chromatography, pre-fabric samples were processed with the method by Palmer (1971) to obtain tissue lipid extracts. According to the results of the study, due to various changes in the content of the entire spectrum of fatty acids of the tissue during the experiment - the use of vicasol causes a slight increase in the unsaturation and the total content of unsaturated fatty acids in the myocardium of geese. These fluctuations are realized depending on the physiological state of the body. where vicasol can stimulate both the biosynthesis processes of individual fatty acids and their mitochondrial and microsomal oxidation, as evidenced by multidirectional reliable changes in the content of their entire spectrum. In particular, on the 21st day, the content of docosopentaenoic acid increased by 36.3% whereas the content of docosohexaenoic and linolenic acids decreased by an average of 21–24%, on the 28th day the content of eicosatetraic and docosahexaenoic acids increased whereas the content of the linoleic acids dropped by 22.6% in control groups. On the 35th day, the content of basic unsaturated fatty acids: palmitooleic, linoleic, linolenic and docosohexaenoic acids increased in the tissue under the influence of vicasol with complete depletion of docosopentaenoic acid. These fluctuations in fatty acid composition cause a slight increase in the total content of unsaturated fatty acids and increase the unsaturation of myocardial lipids on the 28th and 35th days of ontogeny of geese. Based on previous results regarding the antioxidant state of myocardium affected by vicasol and the given findings, which prove changes in the content of the entire spectrum of fatty acids during the selected ontogeny, vicasol can be used in poultry farming as a tool to improve the quality and the resilience of poultry.

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Co-enzyme Q10 and acetyl salicylic acid enhance Hsp70 expression in primary chicken myocardial cells to protect the cells during heat stress

Cited by 23 publications

References 48 publications

Hsp90 Relieves Heat Stress-Induced Damage in Mouse Kidneys: Involvement of Antiapoptotic PKM2-AKT and Autophagic HIF-1α Signaling

Hsp90 Relieves Heat Stress-Induced Damage in Mouse Kidneys: Involvement of Antiapoptotic PKM2-AKT and Autophagic HIF-1α Signaling

Rosemary Reduces Heat Stress by Inducing CRYAB and HSP70 Expression in Broiler Chickens

Жирнокислотний Склад Міокарду Гусей За Дії Вікасолу

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