No abstract

Feeding natural antioxidant supplements to animals and birds has multiple advantages over traditional synthetic vitamins of the antioxidant group. This study investigated the effect of the Avena satíva extract on the antioxidant status and fatty acid composition of liver tissues, the brain, and skeletal muscles of geese, live weight dynamics, and pterylographic parameters during physiological stress of formation of contour and juvenile feathers. It is confirmed that adding oat extract to the geese diet during physiological stress increases tissue antioxidant activity. It was established that during the formation of contour feathers (day 28), the voltage of the antioxidant system is significantly weakened by the action of the extract due to selective inhibition of the synthesis of unsaturated fatty acids, in particular oleic. The synthesis of palmitic and stearic acids is activated. The oat extract caused the most remarkable changes in liver tissues. The subsequent period of formation of juvenile feathers (49 days) is characterized by equalization of the composition of fatty acids in the control and experimental groups. At the end of the experiment, the mass of the geese of the experimental group increased by 17.9% and their pterylographic parameters improved.

Among natural antioxidants, increasing attention is being drawn to avenanthramides - phenolic compounds of the common oat Avena sativa (Linnaeus, 1753). Research has shown that avenanthramides have much higher antioxidant activity than well-known bioflavanoids. Currently, a great deal of work is being conducted on the structure of these compounds and mechanisms of their effect on the organism of humans and animals. We explored the specifics of the influence of aqueous extract from A. satíva on the antioxidant activity and fatty acid composition of lipids of histologically similar tissues of geese with different levels of aerobicity (muscles of the stomach and cardiac muscle), dynamics of the birds’ live weight and pterylographic parameters under physiological loading by the development of contour and juvenile feathers. The addition of extract of oat to the diet of geese during growth of feathers was observed to increase the antioxidant activity of their tissues. Physiological loading related to the development of contour feathers in the examined tissues of geese significantly weakens as a result of selective inhibition of synthesis of unsaturated fatty acids, especially oleic acid, the content of which in 28-day old geese of the experimental group decreased by 31.7 in the cardiac muscle and 46.8 times in the stomach, compared with the control. Further changes in fatty acid composition were characterized by lower number of differences between the control and experimental groups. Increase in antioxidant activity in these tissues during development of juvenile feathers (day 49) occurs as a result of activation of alternative mechanisms of antioxidative protection, which take place with no significant changes in fatty acid composition. Furthermore, we determined that in the stomach and cardiac muscles of geese, the action of extract from common oat activated mechanisms of antioxidative protection, which increased the level of correlation between the changes in fatty acid composition. The study confirmed that the extract caused not only significant increase in the weight of geese at the end of the experiment, but also improved their pterylographic parameters. Therefore, it is practical to conduct similar studies on wild species of birds grown for hunting, because this process of development of feathers, particularly for such species of birds, is essential.

МикросТрукТурные исследования усовершенсТвованных Мясных рубленых полуФабрикаТовРассмотрены усовершенствованные говяжьи фарши с за-меной 5 %, 10 %, 15 % мясной доли на люпиновую муку с добавлением 0,5 % порошка корня девясила, как пряно-аро-матического сырья и контрольный образец говяжьего фарша. Гистологические исследования показали за ШИК реакцией содержание в мясных разработанных полуфабрикатах мясной и растительной части. По гематоксилину и эозину определили процентный состав фарша.ключевые слова: мясные рубленые полуфабрикаты, порошок корня девясила, мука люпина пищевого белого.

Under conditions of physiological stress of different aetiologies, cells of any organism undergo compensatory metabolic changes. The specificity of such changes primarily depends on functional and histochemical features of the tissues of the body. The purpose of this work was to describe the functioning of the main components of the oxidation-reduction system, (ORS, the Krebs cycle, and the antioxidant system) in the myocardium of geese under conditions of the physiological stress occurring during the transition from the embryonic to postnatal development. It has been experimentally established that in the second half of geese embryogenesis, the content of short-chain fatty acids is significantly increased and the content of long-chain polyunsaturated fatty acids in their myocardium decreases. During the first two weeks of the postnatal period, there are reversible changes in the fatty acid composition of this tissue. The second half of the embryogenesis is characterized by the activation of dehydrogenases of the Krebs cycle in the low variability of antioxidant enzyme activity. At the same time, the experiment demonstrated an increase in the antioxidant activity coefficient, with the exception of its significant reduction on the first day of postnatal ontogenesis. The functioning of the ORS in the myocardium of geese is characterized by the presence of correlations between the nature of the changes of 19 investigated components (dehydrogenases of the Krebs cycle, antioxidant enzymes, lipid peroxidation products, antioxidant activity coefficient, fatty acids, their total content and unsaturation) and structuring of the system into two clusters.