Co-electrospun gelatin-chondroitin sulfate/polycaprolactone nanofibrous scaffolds for cartilage tissue engineering

Sharifi, Fereshteh; Irani, Shiva; Azadegan, Golrokh; Pezeshki‐Modaress, Mohamad; Zandi, Mojgan; Saeed, Mahdi

doi:10.1016/j.bcdf.2020.100215

Cited by 31 publications

(22 citation statements)

References 42 publications

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“…The ideal scaffolds should have acceptable mechanical properties that can bare loads at the injured tissues. Moreover, in terms of biochemical property, the scaffolds should be nontoxic to cells, be able to support adhesion of cells, proliferation, can mediate cellto-cell signaling, and maintain phenotype of cells [3,4].…”

Section: Introductionmentioning

confidence: 99%

Poly(ε-caprolactone)/Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Blend from Fused Deposition Modeling as Potential Cartilage Scaffolds

Kosorn

Wutticharoenmongkol

2021

International Journal of Polymer Science

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The scaffolds of poly(ε-caprolactone)/poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PCL/PHBV) blends were fabricated from fused deposition modeling. From indirect cytotoxicity testing based on mouse fibroblasts, all scaffolds with various blend ratios were nontoxic to cells. The surface-treated scaffold with a blend ratio of 25/75 PCL/PHBV exhibited the highest proliferation of porcine chondrocytes and total glycosaminoglycans (GAGs) after 21 days of culture. The scaffolds with a blend ratio of 25/75 with local pores (LP) were prepared from FDM along with a salt leaching technique using NaCl as porogens. The effect of NaOH in surface treatment on the biological property of scaffolds was investigated. The scaffolds with LP and with 1 M NaOH surface treatment exhibited the highest proliferation of cells and total GAGs after 28 days of culture. The degradation behaviors of the scaffolds were studied. The nonsurface treated, surface treated without LP, and surface treated with LP scaffolds were degraded in phosphate buffer (pH 7.4) for 30 days at 37°C and 50°C for nonenzymatic condition and at 37°C for enzymatic condition. The surface treated with LP scaffold showed the highest amount of weight loss, followed by the surface treated without LP, and the nonsurface-treated scaffolds without LP, respectively. The results from Fourier-transform infrared spectroscopy indicated degradation of PCL and PHBV through hydrolysis of the ester functional group. The compressive strengths of all scaffolds were sufficiently high. The results suggested that the scaffolds with the existence of LP and with surface treatment showed the highest potential for use as cartilage scaffolds.

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Section: Introductionmentioning

confidence: 99%

Poly(ε-caprolactone)/Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Blend from Fused Deposition Modeling as Potential Cartilage Scaffolds

Kosorn

Wutticharoenmongkol

2021

International Journal of Polymer Science

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“…In this method, electrostatic forces are used to produce fibers or spheres with different morphologies and sizes at the micrometer and nanometer scales. Electrospun scaffolds can be characterized by their high porosity, good mechanical properties, and flexibility [ 117 , 129 , 130 , 131 , 132 ]. Moreover, the fibers can be modified using this method, e.g., by functionalizing the fiber surface via enzyme immobilization [ 133 , 134 ].…”

Section: Scaffold For Articular Cartilage Repair: Requirements Mamentioning

confidence: 99%

“…Moreover, the fibers can be modified using this method, e.g., by functionalizing the fiber surface via enzyme immobilization [ 133 , 134 ]. Therefore, the fibers obtained by the electrospinning technique have wide biomedical applications in addition to their use as scaffolds in tissue engineering [ 129 , 130 , 131 , 135 ].…”

Section: Scaffold For Articular Cartilage Repair: Requirements Mamentioning

confidence: 99%

Review of Synthetic and Hybrid Scaffolds in Cartilage Tissue Engineering

2020

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Cartilage tissue is under extensive investigation in tissue engineering and regenerative medicine studies because of its limited regenerative potential. Currently, many scaffolds are undergoing scientific and clinical research. A key for appropriate scaffolding is the assurance of a temporary cellular environment that allows the cells to function as in native tissue. These scaffolds should meet the relevant requirements, including appropriate architecture and physicochemical and biological properties. This is necessary for proper cell growth, which is associated with the adequate regeneration of cartilage. This paper presents a review of the development of scaffolds from synthetic polymers and hybrid materials employed for the engineering of cartilage tissue and regenerative medicine. Initially, general information on articular cartilage and an overview of the clinical strategies for the treatment of cartilage defects are presented. Then, the requirements for scaffolds in regenerative medicine, materials intended for membranes, and methods for obtaining them are briefly described. We also describe the hybrid materials that combine the advantages of both synthetic and natural polymers, which provide better properties for the scaffold. The last part of the article is focused on scaffolds in cartilage tissue engineering that have been confirmed by undergoing preclinical and clinical tests.

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“…The scaffold material exhibits excellent antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains and has shown cytocompatibility with human dermal fibroblast cells [39]. Sharifi et al (2020) fabricated gelatin/chondroitin sulfate/polycaprolactone-based nanofibrous scaffolds and has shown better human mesenchymal stem cells (hMSCs) attachment and chondrogenesis differentiation for cartilage tissue engineering applications [40]. The ciprofloxacin-loaded three-dimensional porous phosphate glass-reinforced gelatin scaffolds for bone tissue engineering were synthesized; the scaffolds mimic ECM properties, and sustained release of ciprofloxacin was observed in the phosphate-buffered saline at 37 • C. Cell culture studies for these scaffolds were performed, and the results revealed good cell adhesion and proliferation with enhanced cell viability in osteoblast like MG-63 cells [41].…”

Section: Natural Polymer-based Biomaterialsmentioning

confidence: 99%

Advancement of Nanobiomaterials to Deliver Natural Compounds for Tissue Engineering Applications

Kumar

Abrahamse

2020

IJMS

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Recent advancement in nanotechnology has provided a wide range of benefits in the biological sciences, especially in the field of tissue engineering and wound healing. Nanotechnology provides an easy process for designing nanocarrier-based biomaterials for the purpose and specific needs of tissue engineering applications. Naturally available medicinal compounds have unique clinical benefits, which can be incorporated into nanobiomaterials and enhance their applications in tissue engineering. The choice of using natural compounds in tissue engineering improves treatment modalities and can deal with side effects associated with synthetic drugs. In this review article, we focus on advances in the use of nanobiomaterials to deliver naturally available medicinal compounds for tissue engineering application, including the types of biomaterials, the potential role of nanocarriers, and the various effects of naturally available medicinal compounds incorporated scaffolds in tissue engineering.

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Co-electrospun gelatin-chondroitin sulfate/polycaprolactone nanofibrous scaffolds for cartilage tissue engineering

Cited by 31 publications

References 42 publications

Poly(ε-caprolactone)/Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Blend from Fused Deposition Modeling as Potential Cartilage Scaffolds

Poly(ε-caprolactone)/Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Blend from Fused Deposition Modeling as Potential Cartilage Scaffolds

Review of Synthetic and Hybrid Scaffolds in Cartilage Tissue Engineering

Advancement of Nanobiomaterials to Deliver Natural Compounds for Tissue Engineering Applications

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