Cartilage tissue is under extensive investigation in tissue engineering and regenerative medicine studies because of its limited regenerative potential. Currently, many scaffolds are undergoing scientific and clinical research. A key for appropriate scaffolding is the assurance of a temporary cellular environment that allows the cells to function as in native tissue. These scaffolds should meet the relevant requirements, including appropriate architecture and physicochemical and biological properties. This is necessary for proper cell growth, which is associated with the adequate regeneration of cartilage. This paper presents a review of the development of scaffolds from synthetic polymers and hybrid materials employed for the engineering of cartilage tissue and regenerative medicine. Initially, general information on articular cartilage and an overview of the clinical strategies for the treatment of cartilage defects are presented. Then, the requirements for scaffolds in regenerative medicine, materials intended for membranes, and methods for obtaining them are briefly described. We also describe the hybrid materials that combine the advantages of both synthetic and natural polymers, which provide better properties for the scaffold. The last part of the article is focused on scaffolds in cartilage tissue engineering that have been confirmed by undergoing preclinical and clinical tests.
Three-arm polylactides (PLA) containing
0.2, 7.6, and 13% of d-lactic acid monomeric units were obtained
and refunctionalized
into ATRP macroinitiators via esterification of hydroxyl groups with
2-bromoisobutyryl bromide. These polymeric matrices underwent enzymatic
degradation with various rates and revealed negative results on cytotoxicity
and genotoxicity tests. Camptothecin (CPT), which is an anticancer
active substance, was transformed into acrylic monomers; however,
simple CPT acrylate was not able to polymerization whereas methacrylate
with a linker was ready for FRP and ATRP. The latter monomer was used
for ATRP initiated with various PLA macroinitiators in order to form
block copolymer conjugates of CPT with high load of drug. Based on
kinetic studies at various temperatures, it was found out that the
polymerization stopped at certain monomer conversion because of the
ceiling temperature. The content of CPT in these conjugates was estimated
by means of 1H NMR, quadruple detection array GPC, and
elemental analysis and was in the range 8.0–16.9 wt %. The
products were morphologically heterogeneous, and the shapes and size
of the nano-/microstructures were influenced by crystallinity of the
PLA segment which was shown in AFM images. Terpolymer block conjugates
consisting of addition PEGMA monomeric units were synthesized as well
in order to increase hydrophilicity of the polymers and to protect
a lactone ring in CPT structure. The studies on CPT release were carried
out in vitro and revealed that the rate of CPT discharge was influenced
by the structure of PLA and conjugate composition; however, it was
near to zero-order kinetics. The analysis using the Korsmeyer–Peppas
model suggests that drug release was governed rather according supercase
II transport (n > 1) which shows that it is a
highly
controlled process.
This work was focused on biodegradation with Escherichia coli bacteria studies of PSF-PUR blend semipermeable hollow fiber membranes that possibly can undergo a partial degradation process. Hollow fiber membranes were obtained from polysulfone (PSF) and polyurethane (PUR) containing ester bonds in the polymer chain in various weight ratios using two solvents: N,N-Dimethylmethanamide (DMF) or N-Methylpyrrolidone (NMP). The membranes that underwent the biodegradation process were tested for changes in the ultrafiltration coefficient (UFC), retention and cut-off point. Moreover, the membranes were subjected to scanning electron microscopy (SEM), MeMoExplorerTM Software and Fourier-transform infrared spectroscopy (FT-IR) analysis. The influence of E. coli and its metabolites has been proven by the increase in UFC after biodegradation and changes in the selectivity and porosity of individual membranes after the biodegradation process.
In this study, we focused on obtaining polysulfone-polyurethane (PSF-PUR) blend partly degradable hollow fiber membranes (HFMs) with different compositions while maintaining a constant PSF:PUR = 8:2 weight ratio. It was carried out through hydrolysis, and evaluation of the properties and morphology before and after the hydrolysis process while maintaining a constant cut-off. The obtained membranes were examined for changes in ultrafiltration coefficient (UFC), retention, weight loss, morphology assessment using scanning electron microscopy (SEM) and MeMoExplorer™ Software, as well as using the Fourier-transform infrared spectroscopy (FT-IR) method. The results of the study showed an increase in the UFC value after the hydrolysis process, changes in retention, mass loss, and FT-IR spectra. The evaluation in MeMoExplorer™ Software showed the changes in membranes’ morphology. It was confirmed that polyurethane (PUR) was partially degraded, the percentage of ester bonds has an influence on the degradation process, and PUR can be used as a pore precursor instead of superbly known polymers.
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