Closing microvascular lesions with fibrin sealant-attached muscle pads

Fehm, Nando P.; Vatankhah, Bijan; Dittmar, M.; Tevetoglu, Yesim; Retzl, Gerald; Horn, Markus

doi:10.1002/micr.20165

Cited by 11 publications

(5 citation statements)

References 21 publications

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“…There are other methods to protect a vascular pedicle, e.g., placing adipose tissue fragments or muscle beside it [ 17 ]. This technique of cushioning tissues can be very efficaciously combined with the use of fibrin glue.…”

Section: Discussionmentioning

confidence: 99%

Fibrin glue as a protective tool for microanastomoses in limb reconstructive surgery

Langer

Schildhauer

Dudda

et al. 2015

GMS Interdisciplinary Plastic and Reconstructive Surgery DGPW; 4:Doc14

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Section: Discussionmentioning

confidence: 99%

Fibrin glue as a protective tool for microanastomoses in limb reconstructive surgery

Langer

Schildhauer

Dudda

et al. 2015

GMS Interdisciplinary Plastic and Reconstructive Surgery DGPW; 4:Doc14

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“…A previous study reported limited success in closing microvascular lesions in rats using fibrin sealant [7] . Fibrin sealant, or fibrin glue, is a mixture of fibrinogen, thrombin, and clotting factors which initiates the formation of a fibrin clot at the application site.…”

Section: Additional Informationmentioning

confidence: 97%

Transient middle cerebral artery occlusion with complete reperfusion in spontaneously hypertensive rats

Hill

Nemoto

2014

MethodsX

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Middle cerebral artery occlusion (MCAO) by the intraluminal suture method is widely used to model ischemic stroke in rats. Current methods include transection or ligation of the external carotid or common carotid artery and thus result in partial restoration of perfusion after transient MCAO. Since incomplete reperfusion may influence recovery and thus confound studies of the impact of neuroprotective compounds and therapies on outcomes after stroke, we have devised a novel method to induce transient MCAO with complete reperfusion. Advantages of the method include: MCAO is achieved through insertion of an intraluminal suture into the internal carotid artery through the common carotid artery.At the end of the occlusion period, the suture is withdrawn and the incision in the common carotid artery is closed with cyanoacrylate tissue adhesive and complete reperfusion is established.No residual subcutaneous sutures remain during recovery.Vasculature is restored to the preoperative state.

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“…113 The group utilised Longa's 42 transient iMCAO method in rats, with both filament insertion and MSC injection via a transected ECA, with the CCA temporarily tied for the duration of iMCAO. 113 CCA repair, reported previously to be successful in rats, 114,115 alongside our recent method development in mice, 101,105 would negate the requirement to transect the ECA, therefore, removing the negative welfare outcomes reported following ECA transection. 102,103,114 Although many IA stem cell delivery experimental models utilise rats, there are examples of these studies conducted in mice.…”

Section: Scientific Applications Of Refinementsmentioning

confidence: 98%

A review of experimental models of focal cerebral ischemia focusing on the middle cerebral artery occlusion model

Trotman‐Lucas

Gibson

2021

F1000Res

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Cerebral ischemic stroke is a leading cause of death and disability, but current pharmacological therapies are limited in their utility and effectiveness. In vitro and in vivo models of ischemic stroke have been developed which allow us to further elucidate the pathophysiological mechanisms of injury and investigate potential drug targets. In vitro models permit mechanistic investigation of the biochemical and molecular mechanisms of injury but are reductionist and do not mimic the complexity of clinical stroke. In vivo models of ischemic stroke directly replicate the reduction in blood flow and the resulting impact on nervous tissue. The most frequently used in vivo model of ischemic stroke is the intraluminal suture middle cerebral artery occlusion (iMCAO) model, which has been fundamental in revealing various aspects of stroke pathology. However, the iMCAO model produces lesion volumes with large standard deviations even though rigid surgical and data collection protocols are followed. There is a need to refine the MCAO model to reduce variability in the standard outcome measure of lesion volume. The typical approach to produce vessel occlusion is to induce an obstruction at the origin of the middle cerebral artery and reperfusion is reliant on the Circle of Willis (CoW). However, in rodents the CoW is anatomically highly variable which could account for variations in lesion volume. Thus, we developed a refined approach whereby reliance on the CoW for reperfusion was removed. This approach improved reperfusion to the ischemic hemisphere, reduced variability in lesion volume by 30%, and reduced group sizes required to determine an effective treatment response by almost 40%. This refinement involves a methodological adaptation of the original surgical approach which we have shared with the scientific community via publication of a visualised methods article and providing hands-on training to other experimental stroke researchers.

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Closing microvascular lesions with fibrin sealant-attached muscle pads

Cited by 11 publications

References 21 publications

Fibrin glue as a protective tool for microanastomoses in limb reconstructive surgery

Fibrin glue as a protective tool for microanastomoses in limb reconstructive surgery

Transient middle cerebral artery occlusion with complete reperfusion in spontaneously hypertensive rats

A review of experimental models of focal cerebral ischemia focusing on the middle cerebral artery occlusion model

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