Cloning, sequencing and expression of cDNA encoding growth hormone from Indian catfish (Heteropneustes fossilis)

Abstract

A tissue-specific cDNA library was constructed using polyA+ RNA from pituitary glands of the Indian catfish Heteropneustes fossilis (Bloch) and a cDNA clone encoding growth hormone (GH) was isolated. Using polymerase chain reaction (PCR) primers representing the conserved regions of fish GH sequences the 3' region of catfish GH cDNA (540 bp) was cloned by random amplification of cDNA ends and the clone was used as a probe to isolate recombinant phages carrying the full-length cDNA sequence. The full-length cDNA clone is 1132 bp in length, coding for an open reading frame (ORF) of 603 bp; the reading frame encodes a putative polypeptide of 200 amino acids including the signal sequence of 22 amino acids. The 5' and 3' untranslated regions of the cDNA are 58 bp and 456 bp long, respectively. The predicted amino acid sequence of H. fossils GH shared 98% homology with other catfishes. Mature GH protein was efficiently expressed in bacterial and zebrafish systems using appropriate expression vectors. The successful expression of the cloned GH cDNA of catfish confirms the functional viability of the clone.

Mitochondrial DNA sequence analysis in Anabantoidei fish

“…The sequence of the growth hormone (GH) has become the marker of choice for genetic studies in many fish species since it is an important hormone. GH has been studied extensively, and many of the teleost GH cDNA nucleotide sequences are available [13][14][15]. The GH sequence is only one of the molecular parameters that may aid in phylogenetic analysis within the Anabantoidei suborder.…”

Production and bioactivity test of recombinant protein common carp growth hormone

“…Contoh penggunaan teknologi transgenik adalah penyisipan gen penyandi hormon pertumbuhan (growth hormone (GH)) yang dapat memacu pertumbuhan ikan menjadi beberapa kali lipat dari ikan normal (Lutz, 2001), anti freeze protein (AFP) yang dapat meningkatkan toleransi ikan pada suhu dingin (Beardmore dan Porter, 2003) dan pigmen warna yang dapat menghasilkan ikan hias yang lebih menarik dengan beraneka ragam warna. Sementara itu, teknologi rekombinan dimanfaatkan untuk menghasilkan DNA ataupun protein secara massal yang bermanfaat bagi dengan menggunakan bakteri, ragi, maupun sel sebagai media perbanyakannya (Sekine et al, 1985;Mahmoud 1999;Anathy et al, 2001;Li et al, 2003).…”

A comparative account of the structure of the growth hormone encoding gene and genetic interrelationship in six species of the genus Labeo

“…Besides nucleotide polymorphism, the total number of amino acids found in the GH protein of different species also shows variation. For example GH has 200 amino acids in catfishes (Tang et al 1993;Anathy et al 2001), 204 in tilapia (Rentier-Delrue et al 1989), 207 in L. rohita (Venugopal et al 2002a), and 210 in salmon (Agellon and Chen 1986;Devlin 1993), grass carp, silver carp, and common carp (Ho et al 1989;Chiou et al 1990). …”

“…GH genes isolated from fishes show more variability in their size as well as structure compared to mammals. GH genes with five exons and four introns have been isolated from Chinese carps (Ho et al 1989(Ho et al , 1991Hong and Schartl 1993), common carp (Chiou et al 1990), catfishes (Tang et al 1993;Anathy et al 2001), goldfish (Law et al 1996), mud loach (Noh et al 1999), and blunt snout bream (Li et al 2002), which is similar to the situation in mammals (De Noto et al 1981;Woychik et al 1982; Barta et al 1991) and birds (Buggioti and Primmier 2006). On the other hand GH genes with six exons and five introns have been isolated from rainbow trout (Agellon et al 1988b), tilapias (Rentier-Delrue et al 1989;Ber and Daniel 1993;Sekkali et al 1999), salmon (Devlin 1993), flounder (Sakata et al 1993), sea bass (Yowe and Epping 1995), yellowtail (Ohkubo et al 1996), pufferfish (Venkatesh and Brenner 1997), and seabream (Meiri et al 2004).…”