Cloning of the Coffea canephora SERK1 promoter and its molecular analysis during the cell-to-embryo transition

Jiménez-Guillen, Doribet; Pérez-Pascual, Daniel; Souza-Perera, Ramón; Godoy-Hernández, Gregorio; Zúñiga-Aguilar, José Juan

doi:10.1016/j.ejbt.2018.08.005

Cited by 4 publications

(1 citation statement)

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“…However, in the study by Mishra et al ( 2010 ) no negative effects from introducing the GFP gene were found on the plant regeneration; other researchers also found no negative effect (Pang et al, 1996; Leffel et al, 1997; Ghorbel et al, 1999; Tian et al, 1999; Molinier et al, 2000; Zhu et al, 2004 as cited by Mishra et al, 2010 ). Further A. tumefaciens -mediated transformation experiments on C. canephora mention both the successful introduction of the Bacillus thuringiensis c ry1Ac gene to create a variety resistant to the coffee leaf miner ( Leucoptera coffeella) (Perthuis et al, 2015 ) and the introduction of promoter elements for the CcSERK1 gene to investigate their role during somatic embryogenesis (Jiménez-Guillen et al, 2018 ; Pérez-Pascual et al, 2018 ). Perthuis et al ( 2015 ) confirmed the statement of Mishra et al ( 2010 ) that no growth difference was observed between the non-transformed clones and their transformed clones.…”

Section: Resultsmentioning

confidence: 99%

The biological feasibility and social context of gene-edited, caffeine-free coffee

Leibrock

Santegoets

Mooijman

et al. 2022

Food Sci Biotechnol

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Coffee, especially the species Coffea arabica and Coffea canephora, is one of the world’s most consumed beverages. The consumer demand for caffeine-free coffee is currently being met through chemical decaffeination processes. However, this method leads to loss of beverage quality. In this review, the feasibility of using gene editing to produce caffeine-free coffee plants is reviewed. The genes XMT (7-methylxanthosine methyltransferase) and DXMT (3,7-dimethylxanthine methyltransferase) were identified as candidate target genes for knocking out caffeine production in coffee plants. The possible effect of the knock-out of the candidate genes was assessed. Using Agrobacterium tumefaciens-mediated introduction of the CRISPR-Cas system to Knock out XMT or DXMT would lead to blocking caffeine biosynthesis. The use of CRISPR-Cas to genetically edit consumer products is not yet widely accepted, which may lead to societal hurdles for introducing gene-edited caffeine-free coffee cultivars onto the market. However, increased acceptance of CRISPR-Cas/gene editing on products with a clear benefit for consumers offers better prospects for gene editing efforts for caffeine-free coffee.

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