Cloning and Sequence Analysis of LipL32, a Surface–Exposed Lipoprotein of Pathogenic Leptospira Spp

Darian, Ebrahim Khodaverdi; Forghanifard, Mohammad Mahdi; Bidhendi, Soheila Moradi; Chang, Yung-Fu; Yahaghi, Emad; Esmaelizad, Majid; Khaleghizadeh, Maryam; Khaki, Pejvak

doi:10.5812/ircmj.8793

Cited by 9 publications

(9 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Numerous studies confirmed its high specificity and sensitivity, with the capability of detecting as few as 10 organisms in a sample (46,47,49,50,60). Leptospiral DNA has been detected in body where antibody titres may be lower and appear later than in serum (34).…”

Section: Polymerase Chain Reactionmentioning

confidence: 95%

“…These genes are highly conserved among various pathogenic Leptospira species. Therefore, these genes are useful for detection of leptospiral infection and suitable for designing of positive control in PCR assay (46)(47)(48)(49)(50). Although PCR is now widely used for the diagnosis of many diseases, its general value for the rapid diagnosis of leptospirosis has not been evaluated worldwide as it is not yet widely used, particularly in tropical and subtropical countries.…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

“…The outer membrane protein genes such as lipL32, ompL1, lipL41, ligB, lipL21 genes are highly conserved sequences among pathogenic Leptospires and may be a suitable candidate as recombinant antigen for developing serodiagnostic test such as ELISA (44,(46)(47)(48)(49)(50)(51).…”

Section: Enzyme-linked Immunosorbent Assay Testmentioning

confidence: 99%

See 2 more Smart Citations

Clinical Laboratory Diagnosis of Human Leptospirosis

Khaki

2016

Int J Enteric Pathog

Self Cite

View full text Add to dashboard Cite

Context: Leptospirosis is a worldwide zoonotic infection which appears to be a re-emerging health problem. The clinical features of the disease are broad ranging, but are often similar to those of other infections. As a result, the accuracy of a clinical diagnosis of leptospirosis is low and confirmation requires the use of laboratory tests. Evidence Acquisition: The disease is usually diagnosed in the laboratory by different methods such as direct microscopy, culture, serological methods and molecular methods. The microscopic agglutination test (MAT) is considered the reference test among the several serological methods for leptospirosis diagnosis. However, isolation and identification of the microorganism allows for definitive diagnosis, and provides for epidemiological and prophylactic studies of this disease. Therefore, culture is a golden standard method. Polymerase chain reaction is a rapid, sensitive and specific means of detecting leptospiral infection, in contrast to serology tests. Further benefit is the ability to identify early infection especially during the first few days of the disease even before antibodies are detectable. Conclusions: Choice of test for diagnosis of leptospirosis depends on the stage of the disease. An ideal test will need to discriminate between leptospirosis and a broad spectrum of diseases that cause acute febrile illness and have overlapping clinical presentations. Although detection of antibodies is by itself no proof of a current infection, serological methods (such as MAT and ELISA) are often the most appropriate diagnostic methods.

show abstract

Section: Polymerase Chain Reactionmentioning

confidence: 95%

Section: Polymerase Chain Reactionmentioning

confidence: 99%

See 1 more Smart Citation

Clinical Laboratory Diagnosis of Human Leptospirosis

Khaki

2016

Int J Enteric Pathog

Self Cite

View full text Add to dashboard Cite

show abstract

“…The sequence polymorphisms observed in different strains were silent, suggesting the possibility of an evolutionary pressure to maintain the primary sequence of LipL32 in the pathogen. Serovars isolated from geographically connected areas showed more percentage identity than the sequence from a more distant location [9]. There was nearly 94% sequence similarity observed between the LipL32 sequences of all pathogenic strains studied.…”

Section: The Gene and Proteinmentioning

confidence: 97%

Leptospiral major outer membrane protein

Ghazaei¹

2015

Reviews in Medical Microbiology

View full text Add to dashboard Cite

“…Attempts have been made to develop enzymelinked immunosorbent assays (ELISAs) for detecting Leptospira-specific antibodies using recombinant Leptospira outer-membrane proteins (OMPs) as antigens (5,6,7,8). The LipL32 protein, which is found abundantly in pathogenic Leptospira, is one of the most important OMPs expressed during human infection (9,10). The recombinant proteins produced using Escherichia coli serve as a biologically safe source of antigens, as live Leptospira are pathogenic and pose a risk of infection among laboratory personnel.…”

Section: Introductionmentioning

confidence: 99%

Recombinant LipL32 Protein Developed Using a Synthetic Gene Detects Leptospiraspecific Antibodies in Human Serum Samples

Yaakob

Rodrigues

Opook

et al. 2017

MJMS

View full text Add to dashboard Cite

Background: Synthetic biology is emerging as a viable alternative for the production of recombinant antigens for diagnostic applications. It offers a safe alternative for the synthesis of antigenic principles derived from organisms that pose a high biological risk.Methods: Here, we describe an enzyme-linked immunosorbent assay (ELISA) using the synthetic recombinant LipL32 (rLipL32) protein expressed in Escherichia coli for the detection of Leptospira-specific antibodies in human serum samples. The rLipL32-based ELISA was compared with a microscopic agglutination test (MAT), which is currently used as the gold standard for the diagnosis of leptospirosis.Results: Our results showed that all the MAT-positive serum samples were positive for Leptospira-specific IgG in an ELISA, while 65% (n = 13) of these samples were also positive for Leptospira-specific IgM. In the MAT-negative serum samples, 80% and 55% of the samples were detected as negative by an ELISA for Leptospira-specific IgM and IgG, respectively.Conclusion: An ELISA using the synthetic rLipL32 antigen was able to distinguish Leptospira-specific IgM (sensitivity 65% and specificity 80%) and IgG (sensitivity 100% and specificity 55%) in human serum samples and has the potential to serve as a rapid diagnostic test for leptospirosis.

show abstract

Cloning and Sequence Analysis of LipL32, a Surface–Exposed Lipoprotein of Pathogenic Leptospira Spp

Cited by 9 publications

References 22 publications

Clinical Laboratory Diagnosis of Human Leptospirosis

Clinical Laboratory Diagnosis of Human Leptospirosis

Leptospiral major outer membrane protein

Recombinant LipL32 Protein Developed Using a Synthetic Gene Detects Leptospiraspecific Antibodies in Human Serum Samples

Contact Info

Product

Resources

About