Leptospiral major outer membrane protein

Ghazaei, Ciamak

doi:10.1097/mrm.0000000000000022

Cited by 1 publication

(2 citation statements)

References 43 publications

(52 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LipL32 is the most abundant outer membrane protein (OMP) of leptospiral pathogenic strains. Therefore, LipL32 has been used for the diagnosis of leptospirosis in many studies [24][25][26][27][28][29] and is also used in commercial test kits [45]. In addition, the LipL32-based IgG ELISA has been proven to have sensitivity, specificity, and accuracy comparable to those of the gold standard MAT [28,[45][46][47].…”

Section: Discussionmentioning

confidence: 99%

“…Rapid diagnostic tests, e.g., a lateral flow assay (LFA) or a immunochromatographic assay, are widespread for screening tests because they are user-friendly, low-cost, portable, and do not require special equipment [23]. Many antigens and genes of Leptospira were studied and used for developing diagnostic tests, e.g., LipL21, LigA, Loa22, and LipL32 [24][25][26][27][28][29]. In addition, the proper antigens for developing test kits should allow for differentiation between natural infection and vaccinal immunity.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Leptospiral Leucine-Rich Repeat Protein-Based Lateral Flow for Assessment of Canine Leptospiral Immunoglobulin G

et al. 2022

View full text Add to dashboard Cite

The recombinant, modified leucine-rich repeat protein rhKU_Sej_LRR_2271 has been suggested as a candidate for leptospiral vaccine development since it was predicted to be a transmembrane protein containing leucine-rich repeat motifs and immunogenic epitopes. The immunogenic epitopes showed binding affinities with lower IC50 values than peptides of known antigenic proteins, e.g., LipL32. Moreover, this protein was immunoreactive with hyperimmune sera against several serovars. In this study, we aimed to develop a lateral flow strip test using the rhKU_Sej_LRR_2271 protein for the detection of anti-leptospiral IgG in dogs. The lateral flow assay was performed with 184 dog plasma samples and evaluated with a culture method, 16S ribosomal RNA gene (rss) analysis real-time PCR, and LipL32 ELISA. The culture method failed to detect leptospires in the dog blood samples. Six of nine symptomatic dogs gave positive results with the real-time PCR assay. The lateral flow assay and LipL32 ELISA gave positive results with 59 and 50 dogs, respectively. The sensitivity, specificity, and accuracy of the rhKU_Sej_LRR_2271 lateral flow strip test were 70.00, 82.09, and 78.80%, respectively, when compared with LipL32 ELISA. There was a significant association between the LipL32 ELISA and the rhKU_Sej_LRR_2271 lateral flow assay. The rhKU_Sej_LRR_2271 lateral flow strip test has therefore demonstrated a good potential to detect anti-leptospiral IgG in dogs.

show abstract