Cloning and Overexpression of GmDREB2 Gene from a Vietnamese Drought-resistant Soybean Variety

Tan, Dao Xuan; Tường, Hồ Mạnh; Thuy, Vu Thi Thu; Sơn, Lê Văn; Mậu, Chu Hoàng

doi:10.1590/s1516-89132015050170

Cited by 9 publications

(2 citation statements)

References 23 publications

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“…The GmDREB1 gene has the function of tolerance to heat, drought, salinity, and cold ( Jiang et al, 2014 ). GmDREB2 has been confirmed to increase the drought and salt tolerance of transgenic plants ( Zhang et al, 2013 , Dao et al, 2015 , Pham et al, 2020 ). GmDREB3 enhanced cold, drought, and salt tolerance in transgenic Arabidopsis ( Chen et al, 2009 ); GmDREB5 has been linked to salt tolerance ( Rahmawati et al, 2019 ).…”

Section: Introductionmentioning

confidence: 94%

GmDREB6, a soybean transcription factor, notably affects the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions

Tân

Vaciaxa

et al. 2021

Saudi Journal of Biological Sciences

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Soil is contaminated with salinity, which inhibits plant growth and development and reduces crop yields. The DREB (dehydration responsive element binding protein) gene responds to salt stresses through enhanced transcriptional expression and activation of genes involved in plant salinity resistance. In this study, we present the results of the analysis of the expression of the GmDREB6 transgene, a gene that encodes the soybean DREB6 transcription factor, regulating the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions. The transcription of GmDREB6, NtP5CS , and NtCLC in transgenic tobacco lines was confirmed by qRT-PCR. Under salt stress conditions, the GmDREB6 gene transcription levels in the transgenic tobacco lines L1 and L9 had increased from 2.40- to 3.22- fold compared with the condition without salinity treatment. Two transgenic lines, L1 and L9, had transcription levels of the P5CS gene that had increased from 1.24- to 3.60- fold compared with WT plants. For the NtCLC gene, under salt stress conditions, the transgenic lines had transcription levels that had increased by 3.65–4.54 (fold) compared with WT plants (P < 0.05). The L1-transgenic tobacco line showed simultaneous expression of both the GmDREB6 transgene and two intrinsic genes, the NtP5CS and NtCLC genes. This study demonstrated that expression of the GmDREB6 gene from soybean increases the transcription levels of the NtP5CS and NtCLC genes in transgenic tobacco plants under salt stress conditions. The analysis results have suggested that the GmDREB6 gene is a potential candidate for improving the salt tolerance of plants, opening up research and development opportunities for salt stress-tolerant crops to respond to climate change and the rise in sea levels.

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Section: Introductionmentioning

confidence: 94%

GmDREB6, a soybean transcription factor, notably affects the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions

Tân

Vaciaxa

et al. 2021

Saudi Journal of Biological Sciences

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“…It has also been found that high concentration of Cd induce physiological drought in plant tissues, which results in the activation of pyroline-5-carboxylate enzyme involved in proline biosynthesis(Ali et al, 2001;Delauney and Verma 1993). The positive correlations of free proline with DREB/CBF genes expression suggest that DREB genes might have a role in the biosynthesis of proline in C. sativa plant to Cd stress Tan et al, (2015). also reported a signi cant increase in concentration of free proline in transgenic tobacco plants under the over expression of GmDREB2 gene and drought stress.…”

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confidence: 94%

Molecular and biochemical response of Cannabis sativa plant to cadmium stress and the role of molybdenum

Ali

Hadi

Ali

2023

Preprint

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Plants respond to heavy metals stress by the activation of stress responsive genes to establish biochemical defense mechanisms against the stress. We have investigated the molecular and biochemical response of Cannabis sativa to cadmium (Cd) stress in the presence of molybdenum (Mo) foliar treatments. Various parameters were inter-correlated i.e., Plant biomass, Cd phyto-accumulation, transcript level/expression of CBF/DREB genes, concentrations of free proline, polyphenolics and chlorophyll contents. Phenolic compounds were analyzed by High Performance Liquid Chromatography Diode Array Detector (HPLC-DAD) and Cd contents were analyzed by Atomic Absorption Spectrophotometer. Biomass and chlorophyll contents decreased while the concentrations of free proline and polyphenolics increased under Cd stress. Dry biomass of the all the plant tissues and free proline in roots and leaves were most significantly increased with foliar spray of 2.00 ppm Mo. Nineteen (19) different polyphenolic compounds were identified and quantified in leaves and most of them were highly increased in concentration under Cd stress. The highest concentration was noted in Caffeoyl tyramine (1111 µg/g) in 2.00 ppm Mo treated plants. Transcript level of CBF/DREB genes were less as compared to Mo treated plants, where a high increase in transcript level of these genes were found. Expression of CBF /DREB genes showed significant positive correlations with dry-biomass, Cd accumulation and concentrations of free proline and polyphenolics in leaves. The results suggested that Mo increased the expression of CBF/DREB genes and subsequently the concentration of phenolic compounds and free proline under Cd stress.

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Genetic Transformation in Catharanthus roseus

Nguyen

et al. 2022

Compendium of Plant Genomes

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Cloning and Overexpression of GmDREB2 Gene from a Vietnamese Drought-resistant Soybean Variety

Cited by 9 publications

References 23 publications

GmDREB6, a soybean transcription factor, notably affects the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions

GmDREB6, a soybean transcription factor, notably affects the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions

Molecular and biochemical response of Cannabis sativa plant to cadmium stress and the role of molybdenum

Genetic Transformation in Catharanthus roseus

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