GmDREB6, a soybean transcription factor, notably affects the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions

Abstract

Soil is contaminated with salinity, which inhibits plant growth and development and reduces crop yields. The DREB (dehydration responsive element binding protein) gene responds to salt stresses through enhanced transcriptional expression and activation of genes involved in plant salinity resistance. In this study, we present the results of the analysis of the expression of the GmDREB6 transgene, a gene that encodes the soybean DREB6 transcription factor, regulating the transcription of the NtP5CS and NtCLC genes in transgenic tobacco under salt stress conditions. The transcription of GmDREB6, NtP5CS , and NtCLC in transgenic tobacco lines was confirmed by qRT-PCR. Under salt stress conditions, the GmDREB6 gene transcription levels in the transgenic tobacco lines L1 and L9 had increased from 2.40- to 3.22- fold compared with the condition without salinity treatment. Two transgenic lines, L1 and L9, had transcription levels of the P5CS gene that had increased from 1.24- to 3.60- fold compared with WT plants. For the NtCLC gene, under salt stress conditions, the transgenic lines had transcription levels that had increased by 3.65–4.54 (fold) compared with WT plants (P < 0.05). The L1-transgenic tobacco line showed simultaneous expression of both the GmDREB6 transgene and two intrinsic genes, the NtP5CS and NtCLC genes. This study demonstrated that expression of the GmDREB6 gene from soybean increases the transcription levels of the NtP5CS and NtCLC genes in transgenic tobacco plants under salt stress conditions. The analysis results have suggested that the GmDREB6 gene is a potential candidate for improving the salt tolerance of plants, opening up research and development opportunities for salt stress-tolerant crops to respond to climate change and the rise in sea levels.

Pumpkin CmoDREB2A enhances salt tolerance of grafted cucumber through interaction with CmoNAC1 to regulate H2O2 and ABA signaling and K+/Na+ homeostasis

“…In this study, it was noted that CmoDREB2A positively contributed to the salt tolerance of grafted cucumber ( Fig. 3a–i ), mirroring the findings in tomato [ 17 ], tobacco [ 40 ], Arabidopsis [ 18 , 41 , 42 ], and soybean [ 43 ]. Interestingly, it has been demonstrated that various DREB transcription factors are involved in the salt tolerance in tomato [ 17 ], soybean [ 40 , 43 ], and wheat [ 41 ].…”

“…3a–i ), mirroring the findings in tomato [ 17 ], tobacco [ 40 ], Arabidopsis [ 18 , 41 , 42 ], and soybean [ 43 ]. Interestingly, it has been demonstrated that various DREB transcription factors are involved in the salt tolerance in tomato [ 17 ], soybean [ 40 , 43 ], and wheat [ 41 ]. These findings suggest potential differences in the regulatory mechanisms across different species and highlighting the importance of exploring diverse models.…”

“…In tomato, enhancing its salt tolerance entails overexpressing SlDREB2 [ 17 ]. Similarly, in soybean and tobacco, elevating salt tolerance involves overexpressing GmDREB6 from soybean [ 40 ]. A. thaliana , on the other hand, exhibits improved salt tolerance through overexpression of TaDREB3 from wheat [ 41 ], DREB2A from Pennisetum glaucum [ 42 ], and ScDREB5 from Syntrichia caninervis [ 18 ].…”

S-nitrosylation of SlAPX Is Involved in Alleviating Oxidative Damage in Transgenic Tobacco under Nitrate Stress

“…Previous studies have confirmed that dehydration responsive element binding protein (DREB) is a transcription factor that responds to salt stress by enhancing transcription expression and activating salt tolerance related genes in plants [73]. Under salt stress, transgenic tobacco plants with the soybean GmDREB6 gene increased the transcription levels of GmDREB6 and NtP5CS genes, thus improving salt tolerance [74]. Late embryogenetic abundant (LEA) proteins play an important role in plant responses to abiotic stresses as osmotic regulatory materials and protective materials for cell membrane structure [75].…”

Cấu Trúc Và Hoạt Động Của Vector Chuyển Gen Thực Vật Mang Gen Mã Hoá Choline Oxydase

“…Thuốc lá (Nicotiana tabacum L.) là cây mô hình, được sử dụng để đánh giá hoạt động của vector chuyển gen thực vật và xây dựng quy trình chuyển gen thông qua vi khuẩn A. tumefaciens [14]- [18]. Trong nghiên cứu này, hoạt động của vector biểu hiện mang gen codA đã được đánh giá trong điều kiện hạn sinh lý.…”