Cloning and nucleotide sequencing of Vero toxin 2 variant genes from Escherichia coli O91 : H21 isolated from a patient with the hemolytic uremic syndrome

Genotyping of Shiga-like toxin genes in non-O157 Escherichia coli strains associated with haemolytic uraemic syndrome

“…Much attention has been addressed previously to variant forms of SLT-I1 which were first characterised on a molecular basis in the E. coli 091:H21 strain B2F1 associated with HUS. 15 More recently, SLT-I1 variants have been described in an E. coli 0 1 11 strain from a patient with H U S 7 and in an E. coli OX3 strain isolated from a case of sudden infant death syndrome. 16 Recent epidemiological studies have verified the frequent presence of SLT-II-related toxins among E. coli 0157 isolates.…”

Proposal for effective treatment of Shiga toxin-producing Escherichia coli infection in mice

“…The Stx2d-producing E. coli O91:H21 strain B2F1 was isolated from a human [18] and was a gift from Professor Alison O'Brien (Uniformed Services University of the Health Sciences, USA). Streptomycin (SM)-and mitomycin C (MMC)-resistant E32511/HSC were grown in nutrient agar supplemented with 100 mg/mL of SM (Wako Pure Chemical Industries, Ltd., Osaka, Japan), 0.5 mg/mL of MMC (Kyowa Hakko Kogyo Co., Ltd., Tokyo, Japan).…”

Genotypic variations of Shiga toxin-converting phages from enterohaemorrhagic Escherichia coli O157: H7 isolates

“…The presence of the stx gene in the host strains and in the isolated phages was con®rmed by a PCR assay as described by Lin et al [18], with common primers to detect genes for Stx1 (or VT1 [19]), Stx2 (or VT2 [5]), VT2vha [20], VT2vhb [20], VT2vpl [18] (or SLT-IIv [21]) and VT2vp2 [18] (or SLT-IIva [22]). Subsequently, more speci®c sets of primers were designed to differentiate the stx 1 gene from the stx 2 gene as described by Cebula et al [14].…”