Cloning and genetic variability of aHindIII repetitive DNA inAcrossocheilus paradoxus(Cyprinidae)

Abstract

Thirty clones of a highly repetitive HindIII fragment of DNA from seven populations of Acrossocheilus paradoxus (Cyprinidae) were isolated and sequenced. The fragment represents a tandemly repeated sequence, with a monomeric unit of 270 bp, amounting to 0.08-0.10% of the fish genome. Higher units of this monomer appear as a ladder in Southern blots. The HindIII satellite DNA family is conserved in three genera of the Cyprinidae. Variation in nucleotide sequences of this repetitive fragment, which is A+T-rich, is distributed both within individuals and among populations. High overall nucleotide divergence (dij = 0.056 +/- 0.001) was detected among clones of the HindIII satellite DNAs of Acrossocheilus paradoxus. Based on the molecular clock hypothesis, the maximum evolutionary rate was estimated to be 5.3 x 10(-7) substitutions per site per year. Lineage sorting may have contributed to the genetic heterogeneity within individuals and populations. Cladistic analyses indicated a closer phylogeographic relationship between populations of the central and south regions in Taiwan.

Characterization and genetic variations of satellite DNAs inAcrossocheilus paradoxus(Günther, 1868) (Cyprinidae) indicate population expansion

“…In the species‐specific type I Hin dIII satellite DNA discovered in the A. paradoxus genome by Wu et al (1999), the proportion of the A+T content was higher (64·7%) than in this type II repetitive DNA (52·8%). But type I and II DNAs within A. paradoxus have similar nucleotide divergence averages (0·056 v. 0·055).…”

“…A previously discovered Hin dIII repeated DNA from the genome of A. paradoxus (Wu et al , 1999) is herein named type I satellite DNA and is unique to this species. Phylogeographic analyses indicated a closer relationship between populations of the central and southern region in Taiwan (Wu et al , 1999).…”

“…A previously discovered Hin dIII repeated DNA from the genome of A. paradoxus (Wu et al , 1999) is herein named type I satellite DNA and is unique to this species. Phylogeographic analyses indicated a closer relationship between populations of the central and southern region in Taiwan (Wu et al , 1999). Satellite DNA is a tandemly repetitive element of the genomes of almost all eukaryotic organisms; it is distinguished from minisatellite and microsatellite DNA by the size of the repeat units (Goldstein & Schlötterer, 1999).…”

Conservation of pBuM–2 satellite DNA sequences among geographically isolated Drosophila gouveai populations from Brazil

“…In another study, groups of Acrossocheilus paradoxus (Cyprinidae) populations from Taiwan were also distinguished using sequences of a 270 bp HindIII satellite. These populations were grouped in specific branches in phylogenetic analyses, which is in accordance with the geologic events described on the island (Wu et al, 1999).…”

Genetic variation and population differentiation of Michelia formosana (Magnoliaceae) based on cpDNA variation and RAPD fingerprints: relevance to post-Pleistocene recolonization

“…Since then the topographical barriers have become a natural hindrance to the migration of populations from west to east. Therefore, a very high proportion of species is restricted to the western part of Taiwan (e.g., Miscanthus formosanus, Chou et al 1999;Begonia ravenii, Peng et al 1988; Accrosocheilus paradox, Wu et al 1999). The origin of M. formosana var.…”