Cloning and expression of the Mn-SOD gene from Phascolosoma esculenta

Identification and analysis of icCu/Zn-SOD, Mn-SOD and ecCu/Zn-SOD in superoxide dismutase multigene family of Pseudosciaena crocea

“…In general, the molecular weight of ecCu/ Zn-SOD was higher than icCu/Zn-SOD, which was witnessed in LycSOD1 (15.79 kDa) and LycSOD3 (23.14 kDa) [17]. The subunit molecular weight of LycSOD2 was 25.14 kDa, which was similar to that of the precursor protein of Mn-SOD about 23 kDa [14]. For estimated pI, there was remarkable variance among LycSOD1 (5.50), LycSOD2 (8.36) and LycSOD3 (8.36).…”

“…Considerably, Cu/Zn-SOD has been proved to be a considerable important type in SOD superfamily due to its physiological function and therapeutic potential [12], on the other hands, Cu/Zn-SOD is also one of the most important free radical scavengers responding to oxidative stress [13]. Mn-SOD is regarded as a precursor protein which is produced in mitochondria and plays vital roles in cytoplasm after cleaving the signal peptide [14].…”

Cloning, Expression and Characterization of Mitochondrial Manganese Superoxide Dismutase from the Whitefly, Bemisia tabaci

“…Preliminary studies showed that the overexpression of MnSOD can extend the life span of adult Drosophila melanogaster [27] and reduce oxidative stress in cells [28,29]. Recently, it was found that MnSOD may be involved in immune responses to stimulators, such as heat, coldness, starvation and heavy metals [30,31]. However, until now, information about the function of MnSOD in B. tabaci was still lacking.…”

Alteration of Phascolosoma esculenta heat shock protein 90 expression under heavy metal exposure and thermal stress

“…RNA extraction, cDNA synthesis, polymerase chain reaction (PCR) amplification, and PCR product sequencing were performed according to a procedure described by Wang et al (2010). Two gene-specific primers -P5: 5'-TGCCTGGTCCTCTTTGATGT-3' and P6: 5'-GAGCACCATTTGACATGTTTG-3' -were synthesized to generate the 5'-and 3'-ends of PeHSP90, respectively.…”