Identification and analysis of icCu/Zn-SOD, Mn-SOD and ecCu/Zn-SOD in superoxide dismutase multigene family of Pseudosciaena crocea

Liu, Huihui; He, Jianyu; Chi, Chang-Feng; Gu, Yi-Jie

doi:10.1016/j.fsi.2015.01.032

Cited by 51 publications

(16 citation statements)

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“…The SODs are a ubiquitous family of enzymes that efficiently catalyse the dismutation of superoxide anions [ 44 ] and eliminate excess ROS [ 45 ]. The mRNA levels of SOD s were examined in the present study to determine whether other antioxidant enzymes, in addition to the phase II enzymes (represented by GST s in the present study), were involved in the antioxidant process in the breast muscle of broilers stunned by different methods.…”

Section: Resultsmentioning

confidence: 99%

Gas stunning with CO2 affected meat color, lipid peroxidation, oxidative stress, and gene expression of mitogen-activated protein kinases, glutathione S-transferases, and Cu/Zn-superoxide dismutase in the skeletal muscles of broilers

Zhang

Yue

et al. 2018

J Animal Sci Biotechnol

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BackgroundMeat color and lipid peroxidation are important traits related to meat quality. CO2 concentration is a critical factor that can affect meat quality in the commercial use of gas stunning (GS). However, the effect and mechanism of CO2 stunning on meat color and lipid peroxidation during long-term storage remain poorly studied. We aimed to study the effects of GS methods, especially CO2 concentration, on meat color and meat lipid peroxidation in broilers during long-term storage at 4 °C and to explore the potential mechanism of meat color change via lipid peroxidation and the inner lipid peroxide scavenging system.MethodsEighteen broilers were sacrificed after exposure to one of the following gas mixtures for 90 s: 40% CO2 + 21% O2 + 39% N2 (G40%), 79% CO2 + 21% O2 (G79%), or no stunning (0% CO2, control). Meat color, serum variables, enzyme activities, and the gene expression of mitogen-activated protein kinase (MAPK), nuclear factor-erythroid 2-related factor 2 (Nrf2), glutathione S-transferase (GST) and superoxide dismutase (SOD) were determined.ResultsThe concentrations of serum triiodothyronine (T3, P = 0.03) and the ratio of serum free triiodothyronine/free thyroxine (FT3/FT4, P < 0.01) were decreased, whereas levels of serum cortisol (P < 0.01) were increased in the 40% CO2 group compared with the control group. Additionally, the thiobarbituric acid-reactive substances (TBARS) 3 d (P < 0.01) and TBARS 6 d (P = 0.01) in breast meat and the TBARS 3 d in thigh meat (P < 0.01) were increased in the 40% CO2 group compared with the control group. Serum T3 was negatively correlated with TBARS6 d both in the breast and thigh meat (r = − 0.63, P < 0.01 and r = − 0.47, P = 0.05 respectively). T3/T4 was negatively correlated with TBARS6 d in the breast meat and in the thigh meat (r = − 0.57, P = 0.01; and r = − 0.53, P = 0.03 respectively). Compared with the control group, Lightness (L*) 1 d (P = 0.03) and L*9 d (P < 0.01) were increased, whereas total chromatic aberration (E*) 1 d (P = 0.05) and E*3 d (P < 0.01) were decreased in the breast meat of both the G40% and G79% groups. The values of yellowness (b*) 3 d (P = 0.01), b*6 d (P < 0.01) and E*6 d (P < 0.01) in the thigh meat were lower in both the G40% and G79% groups than in the control group. In the breast muscle, the mRNA levels of c-Jun N-terminal kinase 2 (JNK2, P = 0.03), GSTT1 (P = 0.04), and SOD1 (P = 0.05) were decreased, and the mRNA levels of JNK1 (P = 0.07), Nrf2 (P = 0.09), and GSTA3 (P = 0.06) were slightly lower in both the G40% and G79% groups compared with the control group. However, among these genes, only the mRNA level of JNK1 was decreased in the G40% group compared with the control group and the G79% group (P = 0.03) in the thigh muscle.ConclusionsCompared with the control group, meat color quality in the breast meat was decreased, and the expression of genes in the MAPK/Nrf2/ARE (antioxidant responsive element) antioxidant pathway in breast muscle was partly suppressed by GS of both 40% and 79% CO2. However, oxidative stress an...

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Section: Resultsmentioning

confidence: 99%

Gas stunning with CO2 affected meat color, lipid peroxidation, oxidative stress, and gene expression of mitogen-activated protein kinases, glutathione S-transferases, and Cu/Zn-superoxide dismutase in the skeletal muscles of broilers

Zhang

Yue

et al. 2018

J Animal Sci Biotechnol

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“…It is a good candidate for aquaculture diversification in Mediterranean countries [1,2] due to its fast growth rates [3] and lean flesh [4]. However, in contrast to other Sciaenidae species that are widely farmed in countries such as China [5], meagre culture evidenced variable growth rates [6] and its production is still quite limited (Food and Agriculture Organization of the United Nations, 2015).…”

Section: Introductionmentioning

confidence: 99%

Environmental Salinity Affects Growth and Metabolism in Fingerling Meagre (Argyrosomus Regius)

Ruíz-Jarabo

Tinoco

Vargas‐Chacoff

et al. 2019

Fishes

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The meagre (Argyrosomus regius), a farmed fish in Mediterranean countries, seasonally migrates from offshore areas to estuaries for reproduction. During the first two years of life, the meagre evidences a certain grade of euryhalinity by staying in brackish waters close to the shore. The aim of the present study was to establish if fingerling growth in brackish water is improved compared to seawater, where current culture procedures are conducted. Three-month-old fingerlings were maintained for 45 days under two different salinity regimens (12 and 39 ppt). Several growth parameters as well as osmoregulatory and metabolic variables were assessed. Specific growth rate and hepatosomatic index values revealed that fingerlings performed better in brackish waters (12 ppt) compared to 39 ppt. This study contributes to optimizing meagre rearing conditions, thereby supporting the role of A. regius in aquaculture diversification.

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“…A similar tissue distribution profile of AmMnSOD has been reported for the MnSOD in fish Megalobrama amblycephala (Yih 1955) ( Sun et al, 2014 ) and mollusc Mytilus galloprovincialis (Lamarck 1819) ( Wang et al, 2013 ), in which a higher expression level is observed in liver and muscle. In addition, the spatial expression pattern of AmCu/ZnSOD was similar to that of Cu/ZnSOD in fish Pseudosciaena crocea (Richardson 1846) ( Liu et al, 2015 ) and fish Hypophthalmichthys molitrix (Valenciennes 1844) ( Zhang et al, 2011 ). Therefore, the differential expressions of AmSODs in gill, intestine, kidney, liver and muscle tissues clearly suggested that the expression level of AmSODs was closely related to the antioxidant responses of marbled eels in comparative perspective.…”

Section: Discussionmentioning

confidence: 70%

Effects of salinity change on two superoxide dismutases (SODs) in juvenile marbled eelAnguilla marmorata

Wang

Yin

2016

PeerJ

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Salinity is one of the most important factors that affect the fish growth and survival. Superoxide dismutases (SODs), as the primary antioxidant enzymes, play a first role in the process of preventing oxidative stress caused by excessive superoxide anion (O) in living organisms. In the present study, we investigated the effects of salinity on the gene expressions as well as enzymatic activities of MnSOD and Cu/ZnSOD in gill, intestine, kidney, liver and muscle tissues of the marbled eel Anguilla marmorata. We found that the liver might possess stronger redox capacity compared with other tissues. Furthermore, the gene expressions and enzymatic activities of SODs in juvenile marbled eels could be effectively enhanced by low salinity but inhibited when the salinity was higher than the body tolerance. Our findings indicated that MnSOD and Cu/ZnSOD played vital roles in the adaptation of marbled eels to salinity variation, which contributed to the elucidation of physiological adaptation and regulatory mechanism of SODs in eels.

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Identification and analysis of icCu/Zn-SOD, Mn-SOD and ecCu/Zn-SOD in superoxide dismutase multigene family of Pseudosciaena crocea

Cited by 51 publications

References 38 publications

Gas stunning with CO2 affected meat color, lipid peroxidation, oxidative stress, and gene expression of mitogen-activated protein kinases, glutathione S-transferases, and Cu/Zn-superoxide dismutase in the skeletal muscles of broilers

Gas stunning with CO2 affected meat color, lipid peroxidation, oxidative stress, and gene expression of mitogen-activated protein kinases, glutathione S-transferases, and Cu/Zn-superoxide dismutase in the skeletal muscles of broilers

Environmental Salinity Affects Growth and Metabolism in Fingerling Meagre (Argyrosomus Regius)

Effects of salinity change on two superoxide dismutases (SODs) in juvenile marbled eelAnguilla marmorata

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