Alteration of Phascolosoma esculenta heat shock protein 90 expression under heavy metal exposure and thermal stress

Ch, Li; Xr, Su; Mq, Wang; Li, Yuanyuan; Y, Li; Zhou, Jianli; Tw, Li

doi:10.4238/2012.july.10.14

Cited by 2 publications

(1 citation statement)

References 27 publications

(25 reference statements)

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“…Cd 2 + and Cu 2 + are two major toxic contaminants in marine environment which can generate oxidative stress, DNA damage, apoptosis and protein denature in various living organisms [14] , [17] . The gene expression profiling is frequently used to monitor the impact of heavy metal exposure in diverse vertebrate [12] , [13] , [19] and invertebrate species [1] , [6] , [11] , [21] . Among those diverse animal species, the mussel has been long been used as good sentinel organism that accumulates pollutants in their tissues from the surrounding environment or via food chain [16] .…”

Section: Introductionmentioning

confidence: 99%

Transcriptome response to copper heavy metal stress in hard-shelled mussel ( Mytilus coruscus )

Jiang

Shen

et al. 2016

Genomics Data

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The hard-shelled mussel (Mytilus coruscus) has considerably one of the most economically important marine shellfish worldwide and considered as a good invertebrate model for ecotoxicity study for a long time. In the present study, we used Illumina sequencing technology (HiSeq2000) to sequence, assemble and annotate the transcriptome of the hard-shelled mussel which challenged with copper pollution. A total of 21,723,913 paired-end clean reads (NCBI SRA database SRX1411195) were generated from HiSeq2000 sequencer and 96,403 contigs (with N50 = 1118 bp) were obtained after de novo assembling with Trinity software. Digital gene expression analysis reveals 1156 unigenes are upregulated and 1681 unigenes are downregulated when challenged with copper. By KEGG pathway enrichment analysis, we found that unigenes in four KEGG pathways (aminoacyl-tRNA biosynthesis, apoptosis, DNA replication and mismatch repair) show significant differential expressed between control and copper treated groups. We hope that the gill transcriptome in copper treated hard-shelled mussel can give useful information to understand how mussel handles with heavy metal stress at molecular level.

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