Transcriptome response to copper heavy metal stress in hard-shelled mussel ( Mytilus coruscus )

Abstract

The hard-shelled mussel (Mytilus coruscus) has considerably one of the most economically important marine shellfish worldwide and considered as a good invertebrate model for ecotoxicity study for a long time. In the present study, we used Illumina sequencing technology (HiSeq2000) to sequence, assemble and annotate the transcriptome of the hard-shelled mussel which challenged with copper pollution. A total of 21,723,913 paired-end clean reads (NCBI SRA database SRX1411195) were generated from HiSeq2000 sequencer and 96,403 contigs (with N50 = 1118 bp) were obtained after de novo assembling with Trinity software. Digital gene expression analysis reveals 1156 unigenes are upregulated and 1681 unigenes are downregulated when challenged with copper. By KEGG pathway enrichment analysis, we found that unigenes in four KEGG pathways (aminoacyl-tRNA biosynthesis, apoptosis, DNA replication and mismatch repair) show significant differential expressed between control and copper treated groups. We hope that the gill transcriptome in copper treated hard-shelled mussel can give useful information to understand how mussel handles with heavy metal stress at molecular level.

The Whole-Genome Sequencing and Hybrid Assembly of Mytilus coruscus

“…We used Braker (Hoff et al, 2019) to perform ab initio gene prediction, combining methods that integrate ab initio gene prediction and RNA-seq-based prediction to annotate the protein-coding genes in the M. coruscus genome. These raw RNA-seq reads were downloaded from the EBI for three independent transcriptomic projects, covering multiple tissues and multiple conditions: PRJNA301064 (Xu et al, 2016), PRJNA269003 & PRJNA269004. The resulting predictions were then filtered for the presence of at least one InterPro (Jones et al, 2014) pattern using InterProScan (Mitchell et al, 2019).…”

Assessing relative sensitivity of marine and freshwater bivalves following exposure to copper: Application of classical and novel genotoxicological biomarkers

“…Exposure to metals can induce a variety of detrimental biological effects via a range of mechanisms, including through the generation of reactive oxygen species (ROS). In addition to damage to cellular components such as lipids and proteins, DNA damage (either directly or through generation of ROS), inhibition of DNA repair capacity and disruption of cell cycle control are of particular concern [5][6][7][8][9][10][11][12].…”

Insights into deep‐sea adaptations and host–symbiont interactions: A comparative transcriptome study on Bathymodiolus mussels and their coastal relatives

“…Mussels of the family Mytilidae are widely distributed and have adapted to various habitats from fresh water to the deep sea (Falfushynska et al., ; Fialamedioni, Michalski, Jolles, Alonso, & Montreuil, ; Murgarella et al., ; Xu et al., ). Their adaptive ability to a broad range of environmental stressors makes them good candidates for biomonitoring studies (Matozzo, Chinellato, Munari, Bressan, & Marin, ; Woo, Jeon, Kim, & Yum, ) and has shed light on the adaptive mechanisms to diverse stressors in marine mussels (Leung et al., ).…”

“…Transcriptomic analysis is a powerful approach to reveal mRNA‐level responses of mussels dwelling in diverse habitats (Wang, Liu, & Wu, ; Woo et al., ; Xu et al., ), and also has been widely applied to study symbiotic relationships (Braendle et al., ; Heddi et al., ; Nakabachi et al., ). However, only a few transcriptomic studies have examined host–symbiont interactions in deep‐sea chemosynthetic mussels, and most of them are focused on the physiology of the host (Bettencourt et al., 2010b; Wong et al., ).…”