Cloning and expression of chitin deacetylase 1 from Macrobrachium nipponense , and the effects of dietary protein on growth, body composition and digestive enzymes

Abstract

The optimal protein level in the diet of Oriental river prawn was investigated. Four isocaloric diets containing 300 g/kg, 360 g/kg, 420 g/kg, 480 g/kg crude protein (casein) were tested. Prawns fed 420 g/kg or 480 g/kg protein exhibited higher weight gain, specific growth rate, hepatopancreas somatic indices, feed coefficient and survival rate. Trypsin activity in the hepatopancreas increased with increasing dietary protein, with no significant difference between 420 g/kg and 480 g/kg groups (p < 0.05). Amylase, lipase and cellulase levels were comparable between groups (p > 0.05). Crude protein content in muscle increased with increasing dietary protein, with no significant difference between 420 g/kg and 480 g/kg diets (p > 0.05). There were no significant differences in moisture, crude fat or crude ash between groups. Essential amino acids lysine, tryptophan, phenylalanine, threonine, isoleucine, leucine and valine increased with increasing dietary protein, with no significant difference between 420 g/kg and 480 g/kg groups (p > 0.05). Total and essential amino acids increased with increasing dietary protein. Cloning of the full‐length 2,435 bp Macrobrachium nipponense chitin deacetylase 1 (MnCDA1)gene revealed an open reading frame of 1,632 bp encoding a 535 residue polypeptide qPCR revealed highest expression in the hepatopancreas, followed by muscle, gill, heart and eyestalk. Expression in hepatopancreas and gill tissue, but not muscle, increased gradually with increasing dietary protein.

Comparison of growth performance and biochemical components between parent and hybrid offspring in the oriental river prawn, Macrobrachium nipponense

“…Shrimp are an excellent source of protein, minerals and vitamins, especially essential amino acids such as lysine, tryptophan and phenylalanine (Fan et al 2018). The composition and concentration of amino acids in shrimp muscle may affect its quality (Wang et al 2004).…”

Whole-body transcriptome analysis provides insights into the cascade of sequential expression events involved in growth, immunity, and metabolism during the molting cycle in Scylla paramamosain

“…Finally, the present study identified six chitinases and found that they showed differential expression patterns in stages PoM, InM, and PrM, suggesting different physiological roles and modes of action of during the molting cycle. The chitin deacetylase gene is another chitin metabolism-related gene involved in the catalysis of the acetamido group in the N-acetyl-d-glucosamine units of chitin 49 , 50 . To date, five classes of chitin deacetylase have been characterized, three of which were identified in this study (chitin deacetylase 1, chitin deacetylase 4, and chitin deacetylase 9).…”

Transcriptome Analysis of The Whole Body Provides Novel Insights Into The Molecular Mechanisms of Growth, Immunity, and Metabolism During The Molting Cycle of The Juvenile Mud Crab Scylla Paramamosain

“…Finally, we identi ed six chitinases and found that they showed differential expression patterns in stages PoM, InM, and PrM, suggesting different physiological roles and modes of action of during the molting cycle. The chitin deacetylase gene is another chitin metabolism-related gene involved in the catalysis of the acetamido group in the N-acetyl-d-glucosamine units of chitin [44,45]. To date, ve classes of chitin deacetylase have been characterized, three of which were identi ed in this study (chitin deacetylase 1, chitin deacetylase 4, and chitin deacetylase 9).…”

“…To date, ve classes of chitin deacetylase have been characterized, three of which were identi ed in this study (chitin deacetylase 1, chitin deacetylase 4, and chitin deacetylase 9). In insects, it has been demonstrated that the chitin deacetylase gene is involved not only in growth but also in the immune system [45,46]. However, few studies have charactered the chitin deacetylase genes or studied their function in the molting cycle of crustaceans.…”