Cloning and expression analysis of hif-1α and downstream genes during hypoxic stress in cobia (Rachycentron canadum)

Huang, Jianbo; Amenyogbe, Eric; Yang, Lin-tong; Wang, Zhongliang; Chen, Gang; Wang, Wei-zheng; Zhang, Jiandong

doi:10.1007/s10499-021-00820-4

Cited by 6 publications

(2 citation statements)

References 76 publications

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“…Stress, including heat stress, oxidative stress, or inflammation [32]. Hypoxia inducible factor 1 (HIF-1) and its signaling pathway play an important role in the regulation of high-temperature environments [33], and the expression of the HIF-1 gene can promote its downstream hypoxia-responsive genes, which are crucial for the body's response to low oxygen levels or hypoxia [34]. Under hypoxic response conditions in fish, HIF-1 regulates the transcription of hundreds of genes in a cell-specific expression and acts as a major regulator of many hypoxia-inducible genes under hypoxic conditions [35].…”

Section: Effects Of High-temperature Stress On Fish Stress and Regula...mentioning

confidence: 99%

Transcriptional Modulation Reveals Physiological Responses to Temperature Adaptation in Acrossocheilus fasciatus

Wei

Shi

et al. 2023

IJMS

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In order to explore the molecular regulatory mechanism of temperature acclimation under long-term temperature stress in Acrossocheilus fasciatus, this study used high-throughput sequencing technology to analyze 60 days of breeding under five temperature conditions (12 °C, 16 °C, 20 °C, 24 °C, 28 °C). Compared with 20 °C, 9202, 4959 differentially expressed genes (DEGs) were discovered in low-temperature groups (12 °C, 16 °C), whereas 133 and 878 DEGs were discovered in high-temperature groups (24 °C, 28 °C), respectively. The KEGG functional enrichment analysis revealed that DEGs were primarily enriched in tight junction, PI3 K-Akt signaling pathway and protein digestion and absorption in low-temperature groups, and mainly enriched in proximal tubule bicarbonate reclamation, protein digestion and absorption, and HIF-1 signaling pathway in high-temperature groups. The viability of transcriptome sequencing-based screening of DEGs for temperature adaptation in A. fasciatus was shown by the selection of eight DEGs for further validation by quantitative real-time PCR (qRT-PCR), the findings of which were consistent with the RNA-seq data. According to the findings, protein digestion and absorption were primarily regulated by temperature variations, physiological stress was a significant regulator in regulation under high-temperature stress, and the immune system was a significant regulator in regulation under low-temperature stress. The transcriptional patterns of A. fasciatus under temperature stress are revealed in this study. This knowledge is crucial for understanding how A. fasciatus adapts to temperature and can help us better comprehend the environmental difficulties that A. fasciatus adaptation faces.

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Section: Effects Of High-temperature Stress On Fish Stress and Regula...mentioning

confidence: 99%

Transcriptional Modulation Reveals Physiological Responses to Temperature Adaptation in Acrossocheilus fasciatus

Wei

Shi

et al. 2023

IJMS

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“…Cobia (Rachycentron canadum) is a great potential species for aquaculture. It plays an important role in China's southern coastal marine cage culture industry (Huang et al, 2020;Amenyogbe et al, 2021;Huang et al, 2021;Amenyogbe et al, 2022;Huang et al, 2022). Although the artificial breeding technology of cobia has continued to improve, in the actual process of cobia fry cultivation, the survival rate of cobia larvae was lower during the open-mouth larvae stage due to the short transition time from endogenous nutrition to exogenous nutrition.…”

Section: Introductionmentioning

confidence: 99%

Changes in amino acid and fatty acid composition during early development in cobia (Rachycentron canadum)

Huang

Li²,

Xie³

et al. 2022

Front. Mar. Sci.

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An estimation of the utilization of endogenous nutrients from the yolk sac that occurs during embryonic and early larval development can be a valuable approach to studying the nutritional requirements of fish larvae. In this study, fertilized eggs, embryos (before the membrane, 24 hours after fertilization), newly-hatched larvae (0-d-old), and open-mouthed larvae (3-day-old, unfed rotifers) samples of cobia (Rachycentron canadum) were collected to determine the amino acid and fatty acid profiles. Crude protein composition varies significantly for cobia during the different stages of development. The total amino acids, except for lysine(Lys), histidine (His), methionine(Met), serine(Ser), and glycine(Gly), had no significant change in the early development stage (P > 0.05); the content of other six essential amino acids (EAA) and six non-essential amino acids (NEAA) was consistent with the changing trend of total essential amino acid. From hatching newly hatched larvae to open-mouthed larvae, except for Leu, Phe and Ser, decreased significantly (P<0.05). From the utilization rate of FAA, the utilization rate of Lys and Leu was the highest (60.26%) and (52.21%) in the embryonic and endogenous nutritional stages, respectively. The water content of the open-mouthed larvae of cobia was significantly higher than that of the fertilized eggs, embryos, and newly hatched larvae (P < 0.05). Three kinds of saturated fatty acids (SFA), five kinds of monounsaturated fatty acids (MUFA), and eight kinds of polyunsaturated fatty acids (PUFA) were detected in the dry samples of each developmental stage. Based on these results, juvenile cobia can thrive with a wide range of crude protein concentrations. Further refinement of commercial cobia production feeds would have beneficial consequences for economic and environmental concerns due to the positive impacts of optimizing the protein component as well as the ability to maintain the rapid growth rates. The results show that the larval development of cobia needs to consume large amounts of n3PUFA (especially DHA and EPA) to improve the larvae’s survival rate. Research methods will always have limitations when evaluating the relationship between early life nutrition interventions and well-being outcomes later in life. This study provides essential information regarding cobia larvae’s fatty acid requirements.

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Effects of chronic ammonia nitrogen stress on hydrolases and interleukin 17–3 (IL-17–3) in clam Cyclina sinensis

Ni¹,

Liu²,

Hu³

et al. 2023

Aquacult Int

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Cloning and expression analysis of hif-1α and downstream genes during hypoxic stress in cobia (Rachycentron canadum)

Cited by 6 publications

References 76 publications

Transcriptional Modulation Reveals Physiological Responses to Temperature Adaptation in Acrossocheilus fasciatus

Transcriptional Modulation Reveals Physiological Responses to Temperature Adaptation in Acrossocheilus fasciatus

Changes in amino acid and fatty acid composition during early development in cobia (Rachycentron canadum)

Effects of chronic ammonia nitrogen stress on hydrolases and interleukin 17–3 (IL-17–3) in clam Cyclina sinensis

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