The kiwifruit (Actinidia chinensis) is an economically and nutritionally important fruit crop with remarkably high vitamin C content. Here we report the draft genome sequence of a heterozygous kiwifruit, assembled from ~140-fold next-generation sequencing data. The assembled genome has a total length of 616.1 Mb and contains 39,040 genes. Comparative genomic analysis reveals that the kiwifruit has undergone an ancient hexaploidization event (γ) shared by core eudicots and two more recent whole-genome duplication events. Both recent duplication events occurred after the divergence of kiwifruit from tomato and potato and have contributed to the neofunctionalization of genes involved in regulating important kiwifruit characteristics, such as fruit vitamin C, flavonoid and carotenoid metabolism. As the first sequenced species in the Ericales, the kiwifruit genome sequence provides a valuable resource not only for biological discovery and crop improvement but also for evolutionary and comparative genomics analysis, particularly in the asterid lineage.

Relationships between Gulf of California moisture surges and precipitation in the southwestern United States are examined. Standard surface observations are used to identify gulf surge events at Yuma, Arizona for a multi-year (July-August 1977-2001 period, and CPC precipitation analyses and NCEP/NCAR Reanalysis data are used to relate the gulf surge events to the precipitation and atmospheric circulation patterns, respectively. Emphasis is placed on the relative differences in the precipitation and atmospheric circulation patterns for several categories of surge events, including those that are relatively strong (weak) and those that are accompanied by relatively wet (dry) conditions in Arizona and New Mexico after onset. It is shown that rapid surface dewpoint temperature increases are not necessarily a good indicator of increased rainfall in the region.The extent to which the precipitation and atmospheric circulation patterns are influenced by a phasing of tropical easterly and midlatitude westerly waves is also considered. Results indicate that a significant fraction of the events in all categories are related to the passage of westward propagating tropical easterly waves across western Mexico. However, the occurrence of wet versus dry surges in the southwestern United States is not discriminated by the presence of tropical easterly waves, but rather by the relative location of the upper-level anticyclone in midlatitudes at the time of the gulf surge.

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is one of the most destructive diseases that pose a great threat to wheat production. Wheat landraces represent a rich source of powdery mildew resistance. Here, we report the map-based cloning of powdery mildew resistance gene Pm24 from Chinese wheat landrace Hulutou. It encodes a tandem kinase protein (TKP) with putative kinase-pseudokinase domains, designated WHEAT TANDEM KINASE 3 (WTK3). The resistance function of Pm24 was validated by transgenic assay, independent mutants, and allelic association analyses. Haplotype analysis revealed that a rare 6-bp natural deletion of lysine-glycine codons, endemic to wheat landraces of Shaanxi Province, China, in the kinase I domain (Kin I) of WTK3 is critical for the resistance function. Transgenic assay of WTK3 chimeric variants revealed that only the specific two amino acid deletion, rather than any of the single or more amino acid deletions, in the Kin I of WTK3 is responsible for gaining the resistance function of WTK3 against the Bgt fungus.

Broadband recording systems were adapted to characterize the whistle characteristics of free-ranging Indo-Pacific humpback dolphins (Sousa chinensis) in Sanniang Bay, China. A total of 4630 whistles were recorded, of which 2651 with legible contours and relatively good signal-to-noise ratios were selected for statistical analysis. Of the six tonal types (i.e., flat, down, rise, convex, Ushaped, and sine), flat (N ¼ 1426; 39.45%) was the most predominant, followed by down (N ¼ 754; 23.35%) and rise (N ¼ 489; 12.34%). The whistles showed a short duration (mean 6 SD: 370.19 6 285.61 ms; range: 29-2923 ms), a broad frequency range (fundamental contour ranged from 0.52 to 33 kHz), and two harmonics (mean 6 SD: 1.90 6 2.74, with the maximum frequency of harmonics beyond 96 kHz). Whistles without gaps and stairs accounted for 76.7% and 86.4%, respectively. No significant interspecies differences in frequency parameters were observed compared with S. teuszii, which is inconsistent with morphological taxonomies but confirms phylogenetic results, thus suggesting a close relation between Chinese S. chinensis and Atlantic S. teuszii. Significant intra-and interspecific differences in the genus Sousa were also observed, indicating that animal vocalization may not be limited by genetically determined traits but could also be a function of local habitat adaptation.

Binary code similarity detection, whose goal is to detect similar binary functions without having access to the source code, is an essential task in computer security. Traditional methods usually use graph matching algorithms, which are slow and inaccurate. Recently, neural network-based approaches have made great achievements. A binary function is first represented as an control-flow graph (CFG) with manually selected block features, and then graph neural network (GNN) is adopted to compute the graph embedding. While these methods are effective and efficient, they could not capture enough semantic information of the binary code. In this paper we propose semantic-aware neural networks to extract the semantic information of the binary code. Specially, we use BERT to pre-train the binary code on one token-level task, one block-level task, and two graph-level tasks. Moreover, we find that the order of the CFG's nodes is important for graph similarity detection, so we adopt convolutional neural network (CNN) on adjacency matrices to extract the order information. We conduct experiments on two tasks with four datasets. The results demonstrate that our method outperforms the state-of-art models.

BackgroundFew mitochondrial gene rearrangements are found in vertebrates and large-scale changes in these genomes occur even less frequently. It is difficult, therefore, to propose a mechanism to account for observed changes in mitogenome structure. Mitochondrial gene rearrangements are usually explained by the recombination model or tandem duplication and random loss model.ResultsIn this study, the complete mitochondrial genomes of four flatfishes, Crossorhombus azureus (blue flounder), Grammatobothus krempfi, Pleuronichthys cornutus, and Platichthys stellatus were determined. A striking finding is that eight genes in the C. azureus mitogenome are located in a novel position, differing from that of available vertebrate mitogenomes. Specifically, the ND6 and seven tRNA genes (the Q, A, C, Y, S1, E, P genes) encoded by the L-strand have been translocated to a position between tRNA-T and tRNA-F though the original order of the genes is maintained.ConclusionsThese special features are used to suggest a mechanism for C. azureus mitogenome rearrangement. First, a dimeric molecule was formed by two monomers linked head-to-tail, then one of the two sets of promoters lost function and the genes controlled by the disabled promoters became pseudogenes, non-coding sequences, and even were lost from the genome. This study provides a new gene-rearrangement model that accounts for the events of gene-rearrangement in a vertebrate mitogenome.

Powdery mildew, a fungal disease caused by Blumeria graminis f. sp. tritici (Bgt), has a serious impact on wheat production. Loss of resistance in cultivars prompts a continuing search for new sources of resistance. Wild emmer wheat (Triticum turgidum ssp. dicoccoides, WEW), the progenitor of both modern tetraploid and hexaploid wheats, harbors many powdery mildew resistance genes. We report here the positional cloning and functional characterization of Pm41, a powdery mildew resistance gene derived from WEW, which encodes a coiled-coil, nucleotide-binding site and leucine-rich repeat protein (CNL). Mutagenesis and stable genetic transformation confirmed the function of Pm41 against Bgt infection in wheat. We demonstrated that Pm41 was present at a very low frequency (1.81%) only in southern WEW populations. It was absent in other WEW populations, domesticated emmer, durum, and common wheat, suggesting that the ancestral Pm41 was restricted to its place of origin and was not incorporated into domesticated wheat. Our findings emphasize the importance of conservation and exploitation of the primary WEW gene pool, as a valuable resource for discovery of resistance genes for improvement of modern wheat cultivars.

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