Clinical significance of long non-coding RNA DUXAP8 and its protein coding genes in hepatocellular carcinoma

Abstract

Backgrounds: Hepatocellular carcinoma (HCC) is a lethal malignancy worldwide that is difficult to diagnose during the early stages and its tumors are recurrent. Long non-coding RNAs (lncRNAs) have increasingly been associated with tumor biomarkers for diagnosis and prognosis. This study attempts to explore the potential clinical significance of lncRNA DUXAP8 and its co-expression related protein coding genes (PCGs) for HCC. Method: Data from a total of 370 HCC patients from The Cancer Genome Atlas were utilized for the analysis. DUXAP8 and its top 10 PCGs were explored for their diagnostic and prognostic implications for HCC. A risk score model and nomogram were constructed for prognosis prediction using prognosis-related genes and DUXAP8. Molecular mechanisms of DUXAP8 and its PCGs involved in HCC initiation and progression were investigated. Then, potential target drugs were identified using genome-wide DUXAP8-related differentially expressed genes in a Connectivity Map database. Results: The top 10 PCGs were identified as: RNF2,

Long Non-coding RNA Double Homeobox A Pseudogene 8: A Novel Oncogenic Propellant in Human Cancer

“…expression is markedly upregulated in HCC tumor tissues compared to that in corresponding normal tissues (Yue et al, 2019;Hu et al, 2020;Wang et al, 2020;Wei et al, 2020;Zhang et al, 2020). Jiang et al (2019) and Wang et al (2020) found that higher DUXAP8 expression is strongly associated with poor prognosis in HCC (Wang et al, 2020).…”

“…Emerging evidence suggests that lncRNAs are widely expressed and might function as promising therapeutic targets and prognostic indicators of various diseases and cancers ( Hu et al, 2019 ; Statello et al, 2021 ). DUXAP8 expression is markedly upregulated in HCC tumor tissues compared to that in corresponding normal tissues ( Yue et al, 2019 ; Hu et al, 2020 ; Wang et al, 2020 ; Wei et al, 2020 ; Zhang et al, 2020 ). Jiang et al (2019) and Wang et al (2020) found that higher DUXAP8 expression is strongly associated with poor prognosis in HCC ( Wang et al, 2020 ).…”

“…DUXAP8 is approximately 2,307 bp long. Recent studies have reported that DUXAP8 mRNA is substantially upregulated in many cancer tissues, including pancreatic, bladder, colon, lung, ovarian, and breast cancers ( Jiang et al, 2018 ; Lin et al, 2018 ; Chen et al, 2020a ; He et al, 2020 ; Meng et al, 2020 ; Wang et al, 2020 ; Yang et al, 2021 ), as well as thyroid, hepatocellular and renal cell carcinomas (RCCs) ( Hu et al, 2020 ; Wang et al, 2020 ; Pang and Yang, 2021 ), and glioma, compared to corresponding non-tumor tissues. Emerging literature supports that overexpressed lncRNA DUXAP8 might function as a sponge in cancer tissues, targeting tumor suppressive microRNAs, thereby facilitating target oncogene signaling pathway activity and promoting tumor development and progression.…”

“… Jiang et al (2019) and Wang et al (2020) found that higher DUXAP8 expression is strongly associated with poor prognosis in HCC ( Wang et al, 2020 ). They also showed that DUXAP8 RNA affects mitotic nuclear division, histone binding, regulation of cell cycle phase transitions, oxidative phosphorylation, cell division, and the tricarboxylic acid cycle, indicating that DUXAP8 can act as an oncogene in HCC progression ( Wang et al, 2020 ). Hu et al (2020) also showed that DUXAP8 is substantially upregulated in HCC tissues, and found that DUXAP8 was markedly elevated in advanced stage III/IV tumors compared with stage I/II tumors ( Hu et al, 2020 ).…”

Long non‐coding RNA DUXAP9 promotes hepatocellular carcinoma cell stemness via directly interacting with sox9

“…Notably, there are other DUXAPs which are reported to be involved in tumor progression, such as lncRNA DUXAP10 acts as a diagnostic and prognostic marker for HCC, and promote HCC cell proliferation; 21,22 LncRNA DUXAP8, another DUXAP, also has been shown to serve as a pan‐cancer prognostic marker involved in molecular mechanisms contributing to HCC progression, 23,24 and DUXAP8 promotes HCC cell proliferation and migration by sponging miR‐490‐5p to induce BUB1 expression 25 . Importantly, a recent study has shown that lncRNA DUXAP10 promotes glioma cell stemness through enhancing sox12 mRNA stability 26 .…”