Functions of lncRNA DUXAP8 in non-small cell lung cancer

Wu, Chen; Wu, Song; Wang, Zhongnan; Wang, Bingmei

doi:10.1007/s11033-021-07066-6

Cited by 9 publications

(5 citation statements)

References 100 publications

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“…NSCLC remains to be a malignancy with one of the highest rates of morbidity and mortality in the world (7,23). Pseudogene-derived lncRNAs have been reported to be involved in the occurrence and development of NSCLC (12,24). The lncRNA double homeobox A pseudogene 8, which is partially duplicated from myosin light chain kinase (MYLK), is highly expressed in lung adenocarcinoma and can promote cell progression (25).…”

Section: Discussionmentioning

confidence: 99%

“…Initially, they were dismissed as non-functional genomic junk (11). However, recent studies have revealed that dysregulated pseudogenes, such as double homeobox a pseudogene 8 (DUXAP8) and cathepsin l pseudogene 8 (CTSLP8) can affect oncogenic progression by regulating that of particular cancer-related genes (12,13). In particular, pseudogene-derived long non-coding RNAs (lncRNAs), such as DUXAP8 and KRT17P3 (keratin 17 pseudogene 3), which can function as either oncogenes or tumor suppressors, have been previously implicated in cell proliferation, migration and invasion, in addition to drug resistance in NSCLC (12,14).…”

Section: Introductionmentioning

confidence: 99%

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Pseudogene CSPG4P12 affects the biological behavior of non‑small cell lung cancer by Bcl‑2/Bax mitochondrial apoptosis pathway

et al. 2022

Exp Ther Med

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Increasing evidence has shown that chondroitin sulfate proteoglycan 4 (CSPG4) serve a critical role in tumor progression. However, the roles of chondroitin sulfate proteoglycan 4 pseudogene 12 (CSPG4P12) remain to be elucidated. The present study aimed to investigate the potential effects of CSPG4P12 on the physiological behaviors of non-small cell lung cancer (NSCLC) and its underlying biological mechanism. The expression levels of CSPG4P12 in NSCLC tissues and adjacent normal tissues were analyzed using the gene expression profiling interactive analysis 2 database and reverse transcription-quantitative PCR. Cell Counting Kit-8 and colony formation assays were performed to measure cell proliferation. In addition, Transwell and wound healing assays were performed to assess cell invasion and migration. Cell adhesion was measured by cell-extracellular matrix adhesion assay. Hoechst 33342 staining assay was performed to detect nucleoli of apoptotic cells, and transmission electron microscopy (TEM) was utilized for apoptosis detection. Immunofluorescence and western blot assays were performed to measure the expression levels of apoptosis-related proteins. The present results revealed that the expression levels of CSPG4P12 in NSCLC tissues were significantly lower compared with those in adjacent normal tissues. Overexpression of CSPG4P12 inhibited cell proliferation, invasion, migration and adhesion whilst promoting apoptosis. There were missing mitochondrial cristae and mitochondrial vacuoles in the CSPG4P12-overexpressed cells when observed under TEM. Overexpression of CSPG4P12 also increased the expression of Bax and p53, whereas it inhibited the expression of Bcl2. In conclusion, CSPG4P12 could inhibit NSCLC development and tumorigenesis by activating the p53/Bcl2/Bax mitochondrial apoptotic pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pseudogene CSPG4P12 affects the biological behavior of non‑small cell lung cancer by Bcl‑2/Bax mitochondrial apoptosis pathway

et al. 2022

Exp Ther Med

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“…Moreover, the overexpression of lncRNA DUXAP8 in NSCLC patients is correlated with the poor prognosis. The mechanisms here are diverse including transcriptional, post-transcriptional, and epigenetic regulation ( 46 ). The overexpression of lncRNA CCDC144NL-AS1 in NSCLC patients could promote the proliferation, migration, and invasion of NSCLC cells (H1299, A549, NCI-H650, and HCC827 cells).…”

Section: The Biological Features Of Lung Cancermentioning

confidence: 99%

Current treatments for non-small cell lung cancer

et al. 2022

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Despite improved methods of diagnosis and the development of different treatments, mortality from lung cancer remains surprisingly high. Non-small cell lung cancer (NSCLC) accounts for the large majority of lung cancer cases. Therefore, it is important to review current methods of diagnosis and treatments of NSCLC in the clinic and preclinic. In this review, we describe, as a guide for clinicians, current diagnostic methods and therapies (such as chemotherapy, chemoradiotherapy, targeted therapy, antiangiogenic therapy, immunotherapy, and combination therapy) for NSCLC.

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“…[ 21,22 ] In certain circumstances, radiotherapy may promote tumor cell metastasis and immune escape. [ 23–25 ] For instance, radiotherapy could induce angiogenesis, increase the activity of matrix metalloproteinases, [ 26 ] and enhance the expression of programmed cell death 1 ligand 1 (PD‐L1) in cancer cells. [ 27,28 ] Therefore, the development of novel strategies that could significantly improve the treatment effectiveness of radiotherapy and increase its antitumor immune effects is of great clinical significance.…”

Section: Introductionmentioning

confidence: 99%

Oxygen‐driven cuproptosis synergizes with radiotherapy to potentiate tumor immunotherapy

Pei,

Wang,

Shen

et al. 2024

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The immunological implications of cuproptosis, a form of cell death highly sensitive to oxygen presence, remain largely unexplored in the context of tumor immunotherapy. Herein, we initially investigate the positive correlation between cuproptosis and tumor immunotherapy through bioinformatics analysis. Subsequently, an oxygen generator loaded with copper ions (Cu/APH‐M) has been constructed, which serves as an effective carrier of copper ions and crucially enhances the oxygenation of the tumor microenvironment. Importantly, Cu/APH‐M‐mediated dual strengthening of cuproptosis and radiotherapy could not only trigger a powerful antitumor immunity related to immunogenic cell death by RNA‐sequencing analysis, but also effectively inhibit the growth of both distal and in situ low rectal tumors after combined immunotherapy, creating a robust immune memory effect. Our work reveals the beneficial effects of enhanced cuproptosis in radio‐immunotherapy and elucidates its underlying mechanisms, which provides a novel approach for the synergistic integration of cuproptosis with immunotherapy and radiotherapy, broadening the scope of cuproptosis‐mediated tumor therapy.

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Functions of lncRNA DUXAP8 in non-small cell lung cancer

Cited by 9 publications

References 100 publications

Pseudogene CSPG4P12 affects the biological behavior of non‑small cell lung cancer by Bcl‑2/Bax mitochondrial apoptosis pathway

Pseudogene CSPG4P12 affects the biological behavior of non‑small cell lung cancer by Bcl‑2/Bax mitochondrial apoptosis pathway

Current treatments for non-small cell lung cancer

Oxygen‐driven cuproptosis synergizes with radiotherapy to potentiate tumor immunotherapy

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