Clinical significance of<i>Helicobacter pylori cagA</i>and<i>iceA</i>genotype status

Amjad, Nasser Mohammad; Osman, Hussain Ali; Razak, Najibah Abdul; Kassian, J; Din, Jeffri; Abdullah, Nasuruddin

doi:10.3748/wjg.v16.i35.4443

Cited by 24 publications

(16 citation statements)

References 24 publications

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“…We found an association between H. pylori strains harbouring the iceA2 allele in patients with NUD. This behaviour has also been described in Europe, Saudi Arabia, and Turkey [ 34 ]. Several studies suggest an association of the iceA1 variant and PUD and between iceA2 variants with gastritis [ 34 , 35 ].…”

Section: Discussionsupporting

confidence: 60%

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Prevalence ofHelicobacter pylori vacA,cagA, andiceAGenotypes in Cuban Patients with Upper Gastrointestinal Diseases

Feliciano

Gutiérrez

Valdés

et al. 2015

BioMed Research International

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Virulence factors of Helicobacter pylori can predict the development of different gastroduodenal diseases. There are scarce reports in Cuba about H. pylori isolates genotyping. The aim of the present investigation was to identify allelic variation of the virulence genes vacA, cagA, and iceA in sixty-eight patients diagnosed as H. pylori positive by culture. In seven out of 68 patients, strains from both gastric regions were obtained and considered independent. DNA was extracted from all the H. pylori strains and evaluated by PCR-genotyping. The vacA s1 allele, cagA gene, and iceA2 allele were the most prevalent (72.0%, 56.0%, and 57.3%, respectively). Alleles from m-region showed a similar frequency as s1a and s1b subtypes. The presence of multiple H. pylori genotypes in a single biopsy and two gastric region specimens were found. Significant statistical association was observed between iceA2 allele and patients with non-peptic ulcer dyspepsia (NUD) (P = 0.037) as well as virulence genotypes (s1, s1m2) and patients over 40 years old (P < 0.05). In conclusion, the results demonstrated a high prevalence of H. pylori virulent genotypes in Cuban patients over 40 years old while iceA2 alleles demonstrated a good specificity in patients with NUD.

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Section: Discussionsupporting

confidence: 60%

“…This behaviour has also been described in Europe, Saudi Arabia, and Turkey [ 34 ]. Several studies suggest an association of the iceA1 variant and PUD and between iceA2 variants with gastritis [ 34 , 35 ]. However, this association varies among populations; in Brazil, for instance, iceA1 allele is associated with gastritis [ 31 ].…”

Section: Discussionsupporting

confidence: 60%

Prevalence ofHelicobacter pylori vacA,cagA, andiceAGenotypes in Cuban Patients with Upper Gastrointestinal Diseases

Feliciano

Gutiérrez

Valdés

et al. 2015

BioMed Research International

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“…They found an association between H. pylori strains harboring the iceA2 allele in patients with nonulcer disease. This behavior has also been described in Europe, Saudi Arabia, and Turkey [ 12 ]. However, Huang and his colleagues (2016) revealed no significant association between iceA2 and clinical outcomes [ 1 ].…”

Section: Discussionsupporting

confidence: 59%

“…Furthermore, it was shown that adherence to gastric epithelial cells in vitro stimulates iceA1 transcription [ 11 ]. Several studies suggest an association of the iceA1 variant and PUD [ 12 ]. On the other hand, iceA2 has no homology to known genes, and the function of the iceA2 product remains vague in spite of the fact that this allele is associated with asymptomatic gastritis and nonulcer dyspepsia [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Helicobacter pylori cagA and iceA Genes and Their Association with Gastrointestinal Diseases

Abutaleb

Abdelattef

Abdel-Hady

et al. 2018

International Journal of Microbiology

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H. pylori infection causes peptic ulcer, chronic gastritis, mucosa-associated lymphoid tissue lymphoma, and gastric carcinoma. It has several virulence factors such as cytotoxin-associated gene A(cagA) and the induced by contact with epithelium antigen (iceA). We aimed to explore the relationship between cagA and iceA of H. pylori and gastrointestinal diseases. One hundred and eighteen patients who attended Gastrointestinal Endoscopy Unit at Zagazig University Hospitals, Egypt, were included in this study. Two gastric biopsies were collected and evaluated by rapid urease test (RUT) and PCR. cagA and iceA genes were amplified by PCR. We found that 54 patients (45.76%) were positive by both RUT and PCR. cagA and iceA genes were present in 57.4% and 46.29% of the studied patients, respectively. cagA was the most prevalent gene in gastritis (33.3%) and peptic ulcer (68.7%). iceA1/iceA2 positive genes were the most prevalent in gastric cancer (75%). iceA1 gene was present in 38.7% of cagA positive cases, but iceA2 gene was present in 45.2% of cagA positive cases. iceA1/iceA2 positive genes were present in 29% of cagA positive cases. In conclusion, cagA and iceA genes could be used as markers for severe gastrointestinal diseases. iceA gene was strongly related to cagA gene.

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“…These data are corroborated by the great variability in bacterial load values obtained. In some patients the number of microorganisms in the gastric mucosa was very small and therefore it is unlikely in these cases that the microorganism had an important influence on the patient’s pathology [6,7].…”

Section: Association Between Cag a And Number Of Microorganisms In Tmentioning

confidence: 99%

Relationship between bacterial load, morbidity and cagA gene in patients infected by Helicobacter pylori

Belda¹,

Sáez

Santibáñez

et al. 2012

Clinical Microbiology and Infection

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One hundred and seventy-six biopsies of the gastric corpus and antrum from 97 patients were processed using classical and molecular methods in order to study the relationship between the factor cagA of Helicobacter pylori, bacterial load and morbidity. Bacterial load in patients with cagA was greater than in patients without it, both in the antrum and corpus (p<0.01). There was a statistically significant association between cagA and consumption of proton pump inhibitors (adjusted odds ratio 3.11). Haemorrhage of the upper digestive tract was more associated with bacterial load than with the cagA gene (adjusted odds ratio 2.34 and 1.12, respectively), but none of these associations yielded statistical significance.

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Clinical significance ofHelicobacter pylori cagAandiceAgenotype status

Abstract: Virulence factors were not common in our cohort. The incidence of factors cagA, iceA1 and iceA2 were very low although variations were noted in different ethnic groups.

Cited by 24 publications

References 24 publications

Prevalence ofHelicobacter pylori vacA,cagA, andiceAGenotypes in Cuban Patients with Upper Gastrointestinal Diseases

Prevalence ofHelicobacter pylori vacA,cagA, andiceAGenotypes in Cuban Patients with Upper Gastrointestinal Diseases

Prevalence of Helicobacter pylori cagA and iceA Genes and Their Association with Gastrointestinal Diseases

Relationship between bacterial load, morbidity and cagA gene in patients infected by Helicobacter pylori

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