Clinical significance and functions of microRNA-93/CDKN1A axis in human cervical cancer

Zhang, Xiaofeng; Li, Fengshuang; Zhu, Linzhong

doi:10.1016/j.lfs.2018.08.021

Cited by 20 publications

(13 citation statements)

References 20 publications

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“…MiRNAs belonging to the mir-17-92 have been associated to carcinogenesis, in particular by inhibition of apoptosis via E2F transcription factors, and are therefore referred to as oncomiR-1 cluster [18,19]. In cervical cancer, both miR-20a and miR-93 have been shown contribute to disease progression [20][21][22][23][24]. This indicates that the miRNAs in our 3-miRNA classifier are biologically relevant in cervical carcinogenesis.…”

Section: Discussionmentioning

confidence: 92%

Complementarity between miRNA expression analysis and DNA methylation analysis in hrHPV-positive cervical scrapes for the detection of cervical disease

et al. 2019

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Cervical screening by high-risk HPV (hrHPV) testing requires additional risk stratification (triage), as most infections are transient and only a subset of hrHPV-positive women harbours clinically relevant disease. Molecular triage markers such as microRNAs (miRNAs) and DNA methylation markers are particularly promising, as they can be objectively tested directly on hrHPV-positive scrapes and cervicovaginal self-samples. Here, we evaluated the marker potential of 10 candidate miRNAs in 209 hrHPV-positive scrapes of women with underlying precancer (cervical intraepithelial neoplasia, grade 2–3 (CIN2-3)), cancer, or without disease (CIN0/1). A predictive miRNA classifier for CIN3 detection was built using logistic regression, which was compared to and combined with DNA methylation marker FAM19A4 . Markers were correlated to histology parameters and hrHPV genotype. A miRNA classifier consisting of miR-149, miR-20a, and miR-93 achieved an area under the curve (AUC) of 0.834 for CIN3 detection, which was not significantly different to that of FAM19A4 methylation (AUC: 0.862, p = 0.591). Combining miRNA and methylation analysis demonstrated complementarity between both marker types (AUC: 0.939). While the miRNA classifier seemed more predictive for CIN2, FAM19A4 methylation was particularly high in HPV16-positive and histologically advanced CIN3, i.e. CIN3 with high lesion volume. The miRNA classifier, FAM19A4 methylation, and the miRNA/methylation combination were highest in cancer-associated scrapes. In conclusion, a panel of three miRNAs is discriminatory for CIN3 in hrHPV-positive scrapes and can complement DNA methylation analysis for the efficient detection of cervical disease. Combined analysis of the two marker types warrants further evaluation as triage strategy in hrHPV-based screening.

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Section: Discussionmentioning

confidence: 92%

Complementarity between miRNA expression analysis and DNA methylation analysis in hrHPV-positive cervical scrapes for the detection of cervical disease

et al. 2019

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“…abnormal expression of mirnas has been identified in various types of malignancies (18)(19)(20). Several mirnas have been demonstrated to be involved in cervical cancer initiation and progression (21)(22)(23). mirna-432 (mir-432) dysregulation has been shown to be involved in the carcinogenesis of many cancers (24)(25)(26).…”

Section: Introductionmentioning

confidence: 99%

Circular RNA cSMARCA5 regulates the progression of cervical cancer by acting as a microRNA‑432 sponge

Huang

Yao

et al. 2020

Mol Med Report

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circular rnas (circrnas) have been shown to be involved in the development of cancer. The aim of the present study was to investigate the role of circrna SMarca5 (cSMarca5) in human cervical cancer. in the present study, cSMarca5 expression was upregulated in cervical cancer tissues and cell lines. Furthermore, the proliferation rate of cells transduced with viral plasmids expressing small interfering rna targeting cSMarca5 was downregulated. Bioinformatics analysis predicted that microrna (mir)-432 targeted cSMarca5, and mir-432 was able to interact with epidermal growth factor receptor (eGFr) by binding to its 3'-untranslated region. The expression levels of eGFr, erK1 and erK2 were increased in cervical cancer tissues. Furthermore, correlation analysis revealed that cSMarca5 levels were positively correlated with erK1 and erK2 levels.In conclusion, the present findings suggested that cSMARCA5 may play an important role in the progression of cervical cancer via the erK signaling pathway by modulating mir-432.

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“…This evidence together with previous analyses that established p21 as a target of miR‐93‐5p (http://www.targetscan.org, http://mirdb.org) programmes19 20 led us to perform IHC of the p21 protein that is involved in multiple important functions 31. In fact, previous data showed that miR‐93‐5p, via p21, enhances cell proliferation in nasopharyngeal carcinoma and inhibits apoptosis in hepatocellular carcinoma 32…”

Section: Resultsmentioning

confidence: 80%

miR‐106B‐25 Cluster expression: a comparative human and canine osteosarcoma study

Leonardi¹,

Benassi

Pollino

et al. 2020

Veterinary Record Open

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BackgroundOsteosarcoma (OS) is the most common primary malignant bone tumour in dogs and human beings, characterised by similar genetic and clinical features. With the aim to define similarities and differences in the biological aspects involved in OS progression, a comparative study was performed to create a model to improve patient outcome.MethodsFirst, the expression of microRNAs (miRNAs) belonging to the cluster miR-106b-25 (miR-106b, miR-25 and miR-93-5p) in human and canine OS tissue was compared.ResultsmiR-25 and miR-106b presented a variable expression not significantly different from the corresponding normal bone, while miR-93-5p expression was increased in all OS specimens, with higher levels in the canine subset compared with human. Accordingly, its target p21 presented a weaker and less homogeneous immunostaining distribution in the canine group. Given the high expression of miR-93-5p in all OS specimens, the functional response of human 143B and canine DAN OS cells to miRNA inhibition was evaluated. Although p21 expression increased after miR-93-5p inhibition both at mRNA and protein level, a more significant cell response in terms of proliferation and apoptosis was seen in canine OS cells.ConclusionsIn conclusion, canine OS tissue and cell line presented higher expression levels of miR-93-5p than human OS. In addition, the introduction of miR-93-5p inhibitor caused a cell response in 143B and DAN that differed for the more intense functional impact in the canine OS cell line.

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Clinical significance and functions of microRNA-93/CDKN1A axis in human cervical cancer

Cited by 20 publications

References 20 publications

Complementarity between miRNA expression analysis and DNA methylation analysis in hrHPV-positive cervical scrapes for the detection of cervical disease

Complementarity between miRNA expression analysis and DNA methylation analysis in hrHPV-positive cervical scrapes for the detection of cervical disease

Circular RNA cSMARCA5 regulates the progression of cervical cancer by acting as a microRNA‑432 sponge

miR‐106B‐25 Cluster expression: a comparative human and canine osteosarcoma study

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