miR‐106B‐25 Cluster expression: a comparative human and canine osteosarcoma study

Abstract

BackgroundOsteosarcoma (OS) is the most common primary malignant bone tumour in dogs and human beings, characterised by similar genetic and clinical features. With the aim to define similarities and differences in the biological aspects involved in OS progression, a comparative study was performed to create a model to improve patient outcome.MethodsFirst, the expression of microRNAs (miRNAs) belonging to the cluster miR-106b-25 (miR-106b, miR-25 and miR-93-5p) in human and canine OS tissue was compared.ResultsmiR-25 and miR-106b presented a variable expression not significantly different from the corresponding normal bone, while miR-93-5p expression was increased in all OS specimens, with higher levels in the canine subset compared with human. Accordingly, its target p21 presented a weaker and less homogeneous immunostaining distribution in the canine group. Given the high expression of miR-93-5p in all OS specimens, the functional response of human 143B and canine DAN OS cells to miRNA inhibition was evaluated. Although p21 expression increased after miR-93-5p inhibition both at mRNA and protein level, a more significant cell response in terms of proliferation and apoptosis was seen in canine OS cells.ConclusionsIn conclusion, canine OS tissue and cell line presented higher expression levels of miR-93-5p than human OS. In addition, the introduction of miR-93-5p inhibitor caused a cell response in 143B and DAN that differed for the more intense functional impact in the canine OS cell line.

MiRNAs in Canine and Human Osteosarcoma: A Highlight Review on Comparative Biomolecular Aspects

“…Analysis of the miR-106b cluster (miR-106b, miR-93-5p, and miR-25) in human and canine OS shows variable expression of these molecules. No significant difference with corresponding normal bone was observed in miR-106b and miR-25 expression, while miR-93-5p expression was increased in all OS samples, with higher expression levels in the canine subgroup compared with the human subgroup [ 48 , 49 ] ( Figure 1 ). Zhang et al show that miR-93-5p, via p21, increases cell proliferation in some forms of nasopharyngeal carcinoma, while inhibiting apoptosis in human hepatocellular carcinoma [ 50 ].…”

MicroRNAs as Biomarkers in Canine Osteosarcoma: A New Future?

“…Likewise, an overexpression of miR-93-5p (a miRNA in the same cluster as miR-106b-25) has been reported in human OSA tissues [ 66 , 67 ]. Recently, a study carried out by Leonardi et al (2020) also showed an overexpression of miR-93-5p in canine OSA [ 68 ]. In the same study, the expression of miR-106b and miR-25 were evaluated in dogs, finding no significant differences in their expression profiles when comparing healthy tissues with OSA tissues.…”