Circular RNA cSMARCA5 regulates the progression of cervical cancer by acting as a microRNA‑432 sponge

Abstract

circular rnas (circrnas) have been shown to be involved in the development of cancer. The aim of the present study was to investigate the role of circrna SMarca5 (cSMarca5) in human cervical cancer. in the present study, cSMarca5 expression was upregulated in cervical cancer tissues and cell lines. Furthermore, the proliferation rate of cells transduced with viral plasmids expressing small interfering rna targeting cSMarca5 was downregulated. Bioinformatics analysis predicted that microrna (mir)-432 targeted cSMarca5, and mir-432 was able to interact with epidermal growth factor receptor (eGFr) by binding to its 3'-untranslated region. The expression levels of eGFr, erK1 and erK2 were increased in cervical cancer tissues. Furthermore, correlation analysis revealed that cSMarca5 levels were positively correlated with erK1 and erK2 levels.In conclusion, the present findings suggested that cSMARCA5 may play an important role in the progression of cervical cancer via the erK signaling pathway by modulating mir-432.

The Expression, Functions and Mechanisms of Circular RNAs in Gynecological Cancers

“…However, controversial findings have been reported in another study showing that circSMARCA5 was expressed at higher levels in CC tissues, and knockdown of circSMARCA5 suppressed cellular growth and invasion [102]. The oncogenic functions of circSMARCA5 were mediated through a miR-432/EGFR axis [102]. Besides, the expression of circITCH was significantly decreased in CC tissues compared with the adjacent normal tissues [103].…”

“…Recent data suggest that circSMARCA5 was downregulated in CC tissues compared with adjacent normal tissues, and overexpression of circSMARCA5 suppresses the proliferation and invasion of HeLa cells by sponging miR-620 [82]. However, controversial findings have been reported in another study showing that circSMARCA5 was expressed at higher levels in CC tissues, and knockdown of circSMARCA5 suppressed cellular growth and invasion [102]. The oncogenic functions of circSMARCA5 were mediated through a miR-432/EGFR axis [102].…”

“…High-risk subtypes (16 and 18) of human papillomavirus (HPV) are well established as the causative agents responsible for CC [ 69 ]. Massive studies have shown that a large number of circRNAs were differentially expressed in CC tissues compared with adjacent normal cervical tissues, and play oncogenic or tumor-suppressive roles in the initiation and progression of CC [ 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 ]. All of the circRNAs that are abnormally expressed in CC are summarized in Table 1 , and their functions in CC are shown in Figure 3 .…”

Expression profiling of circular RNAs and their potential role in early‑stage diabetic cardiomyopathy

“…although the alterations in the KeGG pathway may be caused by high levels of blood-glucose in our model, the results still preliminarily suggested that these circrnas may have an association with early stage dcM and the function of these circrnas needs further investigation. numerous studies have shown that circrnas may negatively regulate the inhibitory effects of mirnas on their target mrnas by directly binding to mirnas, thereby acting as mirna sponges (24,35,40,(45)(46)(47). in the present study, mmu_circ_0000652 and mmu_circ_0001058 were found to potentially interact with mir-195 and mir-21, both of which have been shown to serve roles in the metabolism of dcM.…”

“…Numerous studies have shown that circRNAs may negatively regulate the inhibitory effects of miRNAs on their target mRNAs by directly binding to miRNAs, thereby acting as miRNA sponges ( 24 , 35 , 40 , 45 – 47 ). In the present study, mmu_circ_0000652 and mmu_circ_0001058 were found to potentially interact with miR-195 and miR-21, both of which have been shown to serve roles in the metabolism of DCM.…”