Clinical Significance and Analytical Determination of Trace Amounts of Selenium in Human Blood - Spectrophotometeric Technique~!2009-12-24~!2010-03-10~!2010-04-20~!

Abstract

Selenium is an essential trace element that is important in proper immune system functioning and in free radical control. Selenium protects against heart weakness and degeneration. It is crucial for optimal health and detoxification. Selenium is required for the production of thyroid hormones. The body only needs a small amount. Nevertheless, the selenium mineral is an essential mineral which the body requires on a daily basis. The objective of the work concerns the proposal and development of the technique for the measurement of selenium levels in the human blood samples by a simple spectrophotometeric determination in diseased and health conditions.

Selenium and selenoproteins in prostanoid metabolism and immunity

“…Taking cues from our previous research that has indicated the ability of Se at supraphysiological levels to inhibit the viability of CML LSCs (Gandhi et al 2014) involving the endogenous CyPGs as well as the eicosanoid class switching paradigms (Finch et al 2017), we have embarked on targeting the self-renewing and drug resistant LSCs that are responsible for the postremission relapse of AML to achieve the eradication of LSCs as our goal to treat AML using new paradigms. In support of the role of Se therapy in AML, several studies have reported significantly decreased serum levels of Se were observed in patients with AML, CML, acute lymphoblastic leukemia (ALL), and acute nonlymphocytic leukemia (ANLL) (Pazirandeh et al 1999;Hadjibabaie et al 2008;Khanna et al 2010;Stevens et al 2011). These observations imply an inverse causality between serum levels of Se and the progression of hematopoietic malignancies.…”

“…4). Previous clinical studies have emphasized the importance of measurement of baseline Se levels in patients, which is a useful guideline for Se intake (Pazirandeh et al 1999;Hadjibabaie et al 2008;Khanna et al 2010;Stevens et al 2011). However, none of the clinical studies, including the SELECT (Selenium and Vitamin E Cancer Prevention Trial) for prostate cancer, have considered the NSAIDs as a confounding factor to explain if the health benefits of Se supplementation are associated with prostanoids.…”

Protein Damage and Antioxidant Status Alterations Caused by Oxidative Injury in Chronic Myeloid Leukemia

“…Vitamin A and E levels were measured by spectrophotometric standard methods (Bessey et al, 1946;Paterson and Wiggins, 1954;Quaife and Dju, 1949). Selenium levels were measured by the spectrophotometric method using azo dye (Khanna et al, 2010). Magnesium levels were measured using a colorimetric kit based on the calamagite method (Gindler and Heth, 1971).…”

Correlation of serum toll like receptor 9 and trace elements with lipid peroxidation in the patients of breast diseases

“…The total volume was made to 250 L and allowed to stand for 10 min at room temperature. The absorbance of the azo dye was measured at 490 nm against the reagent blank [17,18].…”