Clinical measurement of tumor fluorescence using a new diagnostic system with hematoporphyrin derivative, laser photoradiation, and a spectroscope

Kato, Harubumi; Ono, Junya; Konaka, Chimori; Kawate, Norihiko; Yoneyama, Kazuo; Kinoshita, Komei; Nishimiya, Kensuke; Sakai, Harumasa; Noguchi, Masayuki; Tomono, Takahisa; Hayata, Yoshihiro; Aizawa, Katsuo; Kawasaki, Satoru; Tokuda, Yasuaki

doi:10.1002/lsm.1900040107

Cited by 54 publications

(14 citation statements)

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“…[1]. The most interesting application of that is the noninvasive optical detection of diseases, especially cancer, with the use of a laser induced fluorescence of endogenous biological fluorophores [2,3], what frequently is called an autofluorescent diagnostics (AFD) [4]. One of the typical endogenous fluorophores as well as one of the easily detected fluorophores in vivo is a porphyrin and its derivations (protoporphyrin IX, for example [1]).…”

Section: Introductionmentioning

confidence: 99%

Chronic hypoxia as a factor of enhanced autofluorescence of endogenous porphyrins in soft biological tissues

et al. 2009

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This report, as a continuation of 8-years research on the problem of noninvasive clinical fluorescence diagnostics efficiency, discusses a hypothesis of influence of a chronic hypoxia state in soft alive tissues on the intensity of a laser-induced endogenous porphyrins' autofluorescence in a red region of optical spectra. Earlier this hypothesis was proposed on the basis of analysis of fluorescence activity for erosive-ulcerative impairments of the upper part of a gastrointestinal tract (SPIE Proc., vol. 4613, 2002. -p.286-294). Today the hypothesis additionally is confirmed by means of observation after patients with another illness and by means of analysis of some well-known literature data. An authors' methodology of clinical trails to verify the hypothesis using an up-to-date noninvasive fluorescence diagnostic technique is presented as well. Both theoretical reasons and all new clinical data show that the chronic hypoxia state can be one of the major factors of appearance of a large and abnormal laser-induced autofluorescent signal from biotissues in the spectrum range 600-800 nm, which is associated with abnormally high accumulation of endogenous porphyrins in the tissues. So, the noninvasive autofluorescent diagnostic technique could be a powerful tool to estimate in vivo a chronic hypoxia condition in soft biotissues. For that purpose a classification of chronic hypoxia levels versus in vivo autofluorescence contrast coefficients in tissues is proposed as well.

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Section: Introductionmentioning

confidence: 99%

Chronic hypoxia as a factor of enhanced autofluorescence of endogenous porphyrins in soft biological tissues

et al. 2009

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“…Modified porphyrins have systemically been applied for PDD of tumour tissue since the early 1980s, although the fluorescence signal within the tissue was not significant, making a clinical use of this method difficult (Kato et al, 1984). 10 years ago, aminolevulinic acid (ALA) was found effective for both PDD and photodynamic therapy (PDT) (Kennedy et al, 1990;Gibson et al, 1997;Peng et al, 1997).…”

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confidence: 99%

Photodynamic diagnosis of breast tumours after oral application of aminolevulinic acid

Ladner

Steiner²,

Allemann³

et al. 2001

Br J Cancer

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Photodynamic diagnosis is of increasing interest for diagnosis in oncology. It is based on a more intense incorporation of a fluorescent dye in tumours compared to normal tissue. As a feasibility study we investigated the effectiveness of oral application of 5-aminolevulinic acid for photodynamic diagnosis of human primary mammary tumours. The study included 16 patients with palpable breast tumours. Aminolevulinic acid was administered at a concentration of 40 mg kg −1 bodyweight 150–420 min prior to tumourectomy. Intraoperatively blue light (405 nm) was applied to the operation site. Sections of the excised tumour and some lymph nodes were prepared and analysed with a fluorescent microscope. All primary mammary tumour tissues showed significantly higher fluorescence intensity than surrounding normal mammary tissue. Fluorescence of the mammary tumours could also be discriminated macroscopically and intraoperatively. Fluorescence intensity in nonmetastatic lymph node tissue was higher in 2 out of 3 patients than in primary tumour tissue. By photodynamic diagnosis using aminolevulinic acid we were able to reliably distinguish primary mammary tumours from normal mammary tissue microscopically and macroscopically in all our patients. We suggest that photodynamic diagnosis with aminolevulinic acid for breast tumours should be further investigated and developed for intraoperative use and may well be a simple tool for better intraoperative diagnosis and recognition of tumour margins. We hypothesize that lymph node metastasis of breast tumours will not be detectable by this method. © 2001 Cancer Research Campaign http://www.bjcancer.com

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“…Furthermore, it is clearly documented that both bladder [2] and lung cancer [1,19] can be localized by HpD fluorescence. The real question is whether or not the fluorescent component(s) are the same as the phototoxic components.…”

Section: Fluorescence Versus Photosensitivitymentioning

confidence: 99%

Hematoporphyrin phototherapy of cancer

Berns

Wile

1986

Radiotherapy and Oncology

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Powered by the California Digital Library University of California Radiotherapy and Oncology, 7 (1986) SummaryHematoporphyrin phototherapy of cancer is a new modality for cancer diagnosis and treatment that is currently undergoing clinical trials worldwide. A variety of tumors have been studied, e.g., breast (mostly recurrent skin), lung, bladder, eye, head and neck, gynecological and brain. The most success to date has been with lung and the gynecological tract. Cell and animal studies are being conducted to elucidate the basic photobiological mechanisms involved, as well as the histopathological events associated with tumor destruction. Although major questions remain to be resolved, hematoporphyrin phototherapy is an exciting new therapeutic modality for the treatment of cancer, especially in sites where the unique features of lasers and fiber optics are advantageous.

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Clinical measurement of tumor fluorescence using a new diagnostic system with hematoporphyrin derivative, laser photoradiation, and a spectroscope

Cited by 54 publications

References 13 publications

Chronic hypoxia as a factor of enhanced autofluorescence of endogenous porphyrins in soft biological tissues

Chronic hypoxia as a factor of enhanced autofluorescence of endogenous porphyrins in soft biological tissues

Photodynamic diagnosis of breast tumours after oral application of aminolevulinic acid

Hematoporphyrin phototherapy of cancer

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